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Design, synthesis, and characterization of glycyrrhetinic acid-mediated multifunctional liver-targeting polymeric carrier materials

Published online by Cambridge University Press:  26 May 2020

Qingxia Guan ,
Xue Zhang ,
Yue Zhang ,
Xin Yu ,
Weibing Zhang ,
Liping Wang ,
Shuang Sun ,
Xiuyan Li ,
Yanhong Wang  and
Shaowa Lv
...Show all authors
Qingxia Guan
Affiliation:
College of Pharmacy, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
Xue Zhang
Affiliation:
College of Pharmacy, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
Yue Zhang
Affiliation:
College of Pharmacy, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
Xin Yu
Affiliation:
College of Pharmacy, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
Weibing Zhang
Affiliation:
College of Pharmacy, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
Liping Wang
Affiliation:
College of Pharmacy, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
Shuang Sun
Affiliation:
College of Pharmacy, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
Xiuyan Li
Affiliation:
College of Pharmacy, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
Yanhong Wang
Affiliation:
College of Pharmacy, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
Shaowa Lv*
Affiliation:
College of Pharmacy, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
Yongji Li*
Affiliation:
College of Pharmacy, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
*
a)Address all correspondence to these authors. e-mail: 343405921@qq.com
b)e-mail: gqxwyb@163.com
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Abstract

The purpose of this study was to construct a glycyrrhetinic acid (GA)-mediated, breakable, intracellular, nanoscale drug-delivery carrier via amide and esterification reactions. The structures were identified by Fourier-transformed infrared (FTIR) and 1H-nuclear magnetic resonance (1H-NMR) spectrophotometry. The compatibility and safety of the carrier were evaluated using hemolysis and cytotoxicity tests. The GA-copolymer micelle was prepared using the solvent evaporation method. FTIR and 1H-NMR detection demonstrated the successful construction of the polymer. No hemolysis occurred in any concentration of polymer within 3 h, and the hemolysis rate was less than 5%. 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) experimental results showed that the novel polymer reduced the cell survival rate and had significant cytotoxic effects. The blank nanoparticles were liquid with light blue opalescence. Transmission electron microscopy revealed that the empty micelles were uniform spheres, with an average size of 62 nm and a zeta potential of −13 mV. The novel GA-mediated polymeric carrier material developed here has the potential to effectively kill human SMMC-7721 cancer cells within 3 days when the dose is above 500 ug/mL.

Keywords

hepatocellular carcinomaglycyrrhetinic acidpolymeric carrier materialself-assemblymicelles
Type
Article
Information
Journal of Materials Research , Volume 35 , Issue 10 , 28 May 2020 , pp. 1236 - 1248
Copyright
Copyright © Materials Research Society 2020

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