Abstract
Cyanobacteria are the only prokaryotes that can utilize energy sources from sunlight, electrons from water, carbon from the air, and have the capability of fixing nitrogen. The characterization of various cyanobacteria reveals the performance as well as growth differences when compared among different genus of cyanobacteria, which emphasizes the value of characterization in the cellular grounds. In this paper, we have performed a comparative study on the morphology, growth pattern, physiochemical properties as well as structural features of Spirulina sp. NCIM 5143 and Nostoc ellipsosporum NCIM 2786. The comparison is performed through microscopic images, FESEM and statistical study of cell growth by ANOVA. In silico approach was also carried out on the two enzymes present in both the cyanobacteria, Cyanophycin synthestase (EC: 6.3.2.29) and Nitrate reductase (EC: 1.7.1.1) by studying the physicochemical properties, sequential features and structural modeling. A descriptive profile study was completed on these microorganisms and their proteins that may help to interpret the molecular mechanism of the enzymatic reaction of 1, 3-Propanediol (PDO) production in the future.
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Acknowledgments
The authors are thankful to Central Instrumentation Facility (CIF), Birla Institute of Technology Mesra, Ranchi, Jharkhand for providing all the facilities to carry out this work.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Shreya Anand, Koel Mukherjee and Padmini Padmanabhan. The first draft of the manuscript was written by Shreya Anand and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Anand, S., Mukherjee, K. & Padmanabhan, P. Morphological analysis of Spirulina sp. NCIM 5143 and Nostoc ellipsosporum NCIM 2786 and comparative characterization of associated enzymes through in silico approach. Biologia 75, 1553–1561 (2020). https://doi.org/10.2478/s11756-020-00526-7
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DOI: https://doi.org/10.2478/s11756-020-00526-7