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Autopolyploidy in Chrysanthemum cv. ‘Gongju’ Improved Cold Tolerance

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Abstract

A total of 120 nodal segments of Chrysanthemum ‘Gongju’ plantlets were treated with colchicine to obtain autopolyploidy. Flow cytometry showed that 1 of the 12 surviving seedlings had DNA contents that were twice that of tetraploid plants. Microscopy revealed that the epidermal cell and stomatal area of octoploid plant leaves were larger than those of tetraploid plant leaves. Morphological characteristics showed that the plant height of octoploid plants did not differ from that of tetraploid plants, but their leaves and flowers remarkably varied. The leaves and flowers of octoploid plants were larger than those of tetraploid plants. The diameters of the inflorescence and disc flower of octoploid plants increased by 7.2% and 28.4% compared with those of tetraploid plants, respectively. Similarly, the numbers of their ligulate and tubular flowers increased by 22.6% and 34.0% compared with those of tetraploid plants, respectively. Semi-lethal temperature (LT50) of tetraploid plants was − 8.58 °C, while LT50 of octoploid plants decreased by 3.4 °C. At low temperatures, the relative expression levels of CmICE, CmDREB, CmCOR, CmPYR1, CmFAD, CmWRI, CmBAM, and CmPSP genes related to cold resistance in octoploid plants were higher than those in tetraploid plants. Conversely, the expression levels of CmAPX and CmGSH in octoploid plants were lower than those in tetraploid plants. The relative expression levels of CmOST, CmPDH, and CmOAT did not differ between octoploid and tetraploid plants.

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Funding

This work is supported by Youth Natural Science Foundation of Yanbian University (Yandakehezi (201721)), Research Fund for the Doctoral Program of Yanbian University (2018), Foundation for Department of Education in Jilin Provincial (JJKH20180902KJ), the foundation of Key Laboratory of Landscaping (KF201902), and Ministry of Agriculture and Rural Affairs.

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Authors and Affiliations

  1. College of Agricultural, Yanbian University, Park Road 977, Yanji, 133002, Jilin, China

    Yuanyuan Yue, Manrong Ren, Yingjie Quan, Meilan Lian, Xuanchun Piao, Songquan Wu, Yan Zhou, Meiyu Jin & Ri Gao

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  1. Yuanyuan Yue
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  2. Manrong Ren
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  3. Yingjie Quan
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  7. Yan Zhou
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  8. Meiyu Jin
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  9. Ri Gao
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Contributions

Conceived and designed the experiments: Ri Gao, Meilan Lian, Yuanyuan Yue, and Xuanchun Piao. Performed the experiments: Ri Gao, Yuanyuan Yue, Manrong Ren, Yingjie Quan, Songquan Wu, and Yan Zhou. Analyzed the data: Ri Gao, Yuanyuan Yue, Songquan Wu, and Meiyu Jin. Contributed reagents/materials/analysis tools: Ri Gao and Songquan Wu. Wrote the paper: Ri Gao, Yuanyuan Yue, Manrong Ren, Meilan Lian, and Yingjie Quan. All authors read and approved the final manuscript.

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Correspondence to Ri Gao.

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Key message

The autooctoploids of ‘Gongju’ are induced through colchicine, and autooctoploid plants improved cold resistance by increasing gene expression related to cell membrane and cell osmotic.

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Supplementary Figure S1

Epidermal hair of tetraploid and octoploid ‘Gongju’. (a) Epidermal hair of tetraploid plants. (b) Epidermal hair of octoploid plants. Bars=100 μm. (JPEG 448 kb)

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Yue, Y., Ren, M., Quan, Y. et al. Autopolyploidy in Chrysanthemum cv. ‘Gongju’ Improved Cold Tolerance. Plant Mol Biol Rep 38, 655–665 (2020). https://doi.org/10.1007/s11105-020-01225-y

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