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Identification of Polyphenolic Compounds in Edible Wild Fruits Grown in the North-West of Italy by Means of HPLC-DAD-ESI HRMS

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Abstract

The popularity of edible wild fruits has increased in industrialized countries due to their composition and positive effects. The aim of this study has been to characterize the polyphenolics and anthocyanins of black mulberry (Morus nigra L.), cornelian cherry (Cornus mas L.), elderberry (Sambucus nigra L.), hawthorn (Crataegus monogyna L.), lingonberry (Vaccinium vitis-idaea L.) and rose hip (Rosa canina L.) harvested in the north-west of Italy by means of HPLC-DAD-ESI HRMS in positive ion mode. Although there is an abundant amount of literature related to the polyphenolics of cultivated fruit, a new type of comparison has here been conducted between wild and cultivated fruits on their polyphenolic content. The HPLC-DAD-ESI HRMS method has detected 64 different polyphenolic molecules and it can be used to perform qualitative and quantitative analyses. Furthermore, the cornelian cherry and elderberry samples showed the highest polyphenolic compound levels. The quercetin glycosylated compounds showed the highest percentage of flavonols in most of the analyzed wild fruits.

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References

  1. De Biaggi M, Donno D, Mellano MG, Riondato I, Rakotoniaina EN, Beccaro GL (2018) Cornus mas (L.) fruit as a potential source of natural health-promoting compounds: Physico-chemical characterisation of bioactive components. Plant Foods Hum Nutr 73:89–94

    Article  Google Scholar 

  2. Belwal T, Pandey A, Bhatt ID, Rawal RS, Luo Z (2019) Trends of polyphenolics and anthocyanins accumulation along ripening stages of wild edible fruits of Indian Himalayan region. Sci Rep 9:1–11

    Article  CAS  Google Scholar 

  3. Prior RL, Cao GH, Martin A, Sofic E, McEwen J, O'Brien C, Lischner N, Ehlenfeldt M, Kalt W, Krewer G, Mainland CM (1998) Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity, and variety of Vaccinium species. J Agric Food Chem 46:2686–2693

    Article  CAS  Google Scholar 

  4. Dinda B, Kyriakopoulos AM, Dinda S, Zoumpourlis V, Thomaidis NS, Velegraki A, Markopoulos C, Dinda M (2016) Cornus mas L. (cornelian cherry), an important European and Asian traditional food and medicine: ethnomedicine, phytochemistry and pharmacology for its commercial utilization in drug industry. J Ethnopharmacol 193:670–690

  5. Li CR, Hou XH, Xu YY, Gao W, Li P, Yang H (2020) Manual annotation combined with untargeted metabolomics for chemical characterization and discrimination of two major crataegus species based on liquid chromatography quadrupole time-of-flight mass spectrometry. J Chromatogr A 1612(46):06–28

    Google Scholar 

  6. Šamec D, Piljac-Žegarac J (2011) Postharvest stability of antioxidant compounds in hawthorn and cornelian cherries at room and refrigerator temperatures - comparison with blackberries, white and red grapes. Sci Hortic 131:15–21

    Article  Google Scholar 

  7. Narwojsz A, Tańska M, Mazur B, Borowska EJ (2019) Fruit physical features, phenolic compounds profile and inhibition activities of cranberry cultivars (Vaccinium macrocarpon) compared to wild-grown cranberry (Vaccinium oxycoccus). Plant Foods Hum Nutr 74:300–306

    Article  CAS  Google Scholar 

  8. Liu J, Hefni ME, Witthöft CM (2020) Characterization of flavonoid compounds in common Swedish berry species. Foods 9(3):358

    Article  CAS  Google Scholar 

  9. Fan ZL, Wang ZY, Liu JR (2011) Cold-field fruit extracts exert different antioxidant and antiproliferative activities in vitro. Food Chem 129:402–407

  10. Sánchez-Salcedo EM, Mena P, García-Viguera C, Martínez JJ, Hernández F (2015) Phytochemical evaluation of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits, a starting point for the assessment of their beneficial properties. J Funct Foods 12:399–408

    Article  Google Scholar 

  11. Ieri F, Innocenti M, Possieri L, Gallori S, Mulinacci N (2015) Phenolic composition of “bud extracts” of Ribes nigrum L., Rosa canina L. and Tilia tomentosa M. J Pharm Biomed Anal 115:1–9

    Article  CAS  Google Scholar 

  12. Borges G, Degeneve A, Mullen W, Crozier A (2009) Identification of flavonoid and phenolic antioxidants in black currants, blueberries, raspberries, red currants, and cranberries. J Agric Food Chem 58:3901–3909

    Article  Google Scholar 

  13. Wang X, Tong H, Chen F, Gangemi JD (2010) Chemical characterization and antioxidant evaluation of muscadine grape pomace extract. Food Chem 123:1156–1162

    Article  CAS  Google Scholar 

  14. Zorzi M, Gai F, Medana C, Aigotti R, Morello S, Peiretti PG (2020) Bioactive compounds and antioxidant activity of small berries. Foods 9(5):623

  15. Jang GH, Kim HW, Lee MK, Jeong SY, Bak AR, Lee DJ, Kim JB (2018) Characterization and quantification of flavonoid glycosides in the Prunus genus by UPLC-DAD-QTOF/MS. Saudi J Biol Sci 25:1622–1631

    Article  CAS  Google Scholar 

  16. Schrimpe-Rutledge AC, Codreanu SG, Sherrod SD, McLean JA (2016) Untargeted metabolomics strategies – challenges and emerging directions. J Am Soc Mass Spectrom 27(12):1897–1905

    Article  CAS  Google Scholar 

  17. Svedström U, Vuorela H, Kostiainen R, Huovinen K, Laakso I, Hiltunen R (2002) High-performance liquid chromatographic determination of oligomeric procyanidins from dimers up to the hexamer in hawthorn. J Chromatogr A 968:53–60

    Article  Google Scholar 

  18. Jurikova T, Sochor J, Rop O, Mlcek J, Balla S, Szekeres L, Adam V, Kizek R (2012) Polyphenolic profile and biological activity of Chinese hawthorn (Crataegus pinnatifida Bunge) fruits. Molecules 17:14490–14509

    Article  CAS  Google Scholar 

  19. Ek S, Kartimo H, Mattila S, Tolonen A (2006) Characterization of phenolic compounds from lingonberry (Vaccinium vitis-idaea). J Agric Food Chem 54:9834–9842

    Article  CAS  Google Scholar 

  20. Gundogdu M, Muradoglu F, Sensoy RG, Yilmaz H (2011) Determination of fruit chemical properties of Morus nigra L., Morus alba L. and Morus rubra L. by HPLC. Sci Hortic 132:37–41

    Article  CAS  Google Scholar 

  21. Hvattum E (2002) Determination of phenolic compounds in rose hip (Rosa canina) using liquid chromatography coupled to electrospray ionisation tandem mass spectrometry and diode-array detection. Rapid Commun Mass Spectrom 16:655–662

    Article  CAS  Google Scholar 

  22. Lee J, Finn CE (2007) Anthocyanins and other polyphenolics in American elderberry (Sambucus canadensis) and European elderberry (S. nigra) cultivars. J Sci Food Agric 87:2665–2675

    Article  CAS  Google Scholar 

  23. Pawlowska AM, Camangi F, Braca A (2010) Quali-quantitative analysis of flavonoids of Cornus mas L. (Cornaceae) fruits. Food Chem 119:1257–1261

    Article  CAS  Google Scholar 

  24. Adamczak A, Buchwald W, Zieliński J, Mielcarek S (2012) Flavonoid and organic acid content in rose hips (Rosa L., sect. Caninae DC. EM. Christ.). Acta Biol Cracov Ser Bot 54:105–112

    Google Scholar 

  25. Bujor OC, Ginies C, Popa VI, Dufour C (2018) Phenolic compounds and antioxidant activity of lingonberry (Vaccinium vitis-idaea L.) leaf, stem and fruit at different harvest periods. Food Chem 252:356–365

    Article  CAS  Google Scholar 

  26. Mikulic-Petkovsek M, Slatnar A, Stampar F, Veberic R (2012) HPLC-MSn identification and quantification of flavonol glycosides in 28 wild and cultivated berry species. Food Chem 135:2138–2146

    Article  CAS  Google Scholar 

  27. Feng C, Su S, Wang L, Wu J, Tang Z, Xu Y, Shu Q, Wang L (2016) Antioxidant capacities and anthocyanin characteristics of the black-red wild berries obtained in Northeast China. Food Chem 204:150–158

    Article  CAS  Google Scholar 

  28. Veberic R, Slatnar A, Bizjak J, Stampar F, Mikulic-Petkovsek M (2015) Anthocyanin composition of different wild and cultivated berry species. LWT-Food Sci Technol 60:509–517

  29. Veberic R, Jakopic J, Stampar F, Schmitzer V (2009) European elderberry (Sambucus nigra L.) rich in sugars, organic acids, anthocyanins and selected polyphenols. Food Chem 114:511–515

    Article  CAS  Google Scholar 

  30. Barros L, Carvalho AM, Ferreira IC (2011) Comparing the composition and bioactivity of Crataegus monogyna flowers and fruits used in folk medicine. Phytochem Anal 22:181–188

    Article  CAS  Google Scholar 

  31. Wenzig EM, Widowitz U, Kunert O, Chrubasik S, Bucar F, Knauder E, Bauer R (2008) Phytochemical composition and in vitro pharmacological activity of two rose hip (Rosa canina L.) preparations. Phytomedicine 15:826–835

    Article  CAS  Google Scholar 

  32. Özgen M, Serçe S, Kaya C (2009) Phytochemical and antioxidant properties of anthocyanin-rich Morus nigra and Morus rubra fruits. Sci Hortic 119:275–279

    Article  Google Scholar 

  33. Giovanelli G, Buratti S (2009) Comparison of polyphenolic composition and antioxidant activity of wild Italian blueberries and some cultivated varieties. Food Chem 112:903–908

    Article  CAS  Google Scholar 

  34. Çekiç C, Özgen M (2010) Comparison of antioxidant capacity and phytochemical properties of wild and cultivated red raspberries (Rubus idaeus L.). J Food Compos Anal 23:540–544

    Article  Google Scholar 

  35. Pantelidis GE, Vasilakakis M, Manganaris GA, Diamantidis GR (2007) Antioxidant capacity, phenol, anthocyanin and ascorbic acid contents in raspberries, blackberries, red currants, gooseberries and cornelian cherries. Food Chem 102:777–783

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the Fondazione Cassa di Risparmio di Torino [grant number 2015.0488]. The author would like to thank Dr. Sara Morello for her technical support.

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Correspondence to Francesco Gai.

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Zorzi, M., Gai, F., Medana, C. et al. Identification of Polyphenolic Compounds in Edible Wild Fruits Grown in the North-West of Italy by Means of HPLC-DAD-ESI HRMS. Plant Foods Hum Nutr 75, 420–426 (2020). https://doi.org/10.1007/s11130-020-00830-2

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