Skip to main content
SpringerLink
Log in

Growth-promoting and disease-suppressing effects of Paenibacillus polymyxa strain YCP16-23 on pepper (Capsicum annuum) plants

  • Original Article
  • Published:
Tropical Plant Pathology Aims and scope Submit manuscript

Abstract

Phytophthora blight is the most devastating disease for pepper (Capsicum annuum L.) production and is caused by Phytophthora capsici. Current management strategies are not effective. Our study assessed the multi-trait effects of Paenibacillus polymyxa, which suppresses Phytophthora blight and promotes plant growth in pepper plants. A total of 82 bacterial strains isolated from rhizosphere soil of pepper plants were screened for in vitro inhibition of P. capsici growth. The results revealed that only six strains showed strong antagonistic activity against P. capsici. These six strains were used for controlling Phytophthora blight in pepper by seedling assays under greenhouse conditions. Three strains showed a lower incidence of Phytophthora blight and were used in further growth-promoting and biocontrol studies under greenhouse conditions. Strain YCP16-23 showed >60.0% suppression of P. capsici and significantly promoted growth of pepper plants compared to the untreated control. Based on cultural and morphological characterization and 16S rRNA gene sequencing, strain YCP16-23 was identified as Paenibacillus polymyxa. Strain YCP16-23 demonstrated many characteristics that are beneficial for plants, such as production of α-amylase, ammonia, cellulase, indole-3-acetic acid, pectinase, siderophores, and protease, as well as solubilization of phosphate. In a two-year field trial, strain YCP16-23 exhibited remarkable disease suppressing effect by controlling Phytophthora blight (76.24% and 38.09% in years 2017 and 2018, respectively) compared with the untreated control. Strain YCP16-23 caused disease suppression comparable to the microbial fungicides ‘Cike’ (72.04% and 15.41%) and Fluazinam (80.47% and 5.24%). Overall, the results suggest that strain YCP16-23 could provide protection against Phytophthora blight via direct and indirect modes of action and can be used as a biocontrol agent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Adesemoye AO, Kloepper JW (2009) Plant-microbes interactions in enhanced fertilizer use efficiency. Applied Microbiology and Biotechnology 85:1–12

    CAS  PubMed  Google Scholar 

  • Adesemoye AO, Torbert HA, Kloepper JW (2009) Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers. Microbial Ecology 58:921–929

    CAS  PubMed  Google Scholar 

  • Batista BD, Lacava PT, Ferrari A, Teixeira-Silva NS, Bonatelli ML, Tsui S, Mondin M, Kitajima EW, Pereira JO, Azevedo JL, Quecine MC (2018) Screening of tropically derived, multi-trait plant growth-promoting rhizobacteria and evaluation of corn and soybean colonization ability. Microbiological Research 206:33–42

    PubMed  Google Scholar 

  • Bhattacharyya PN, Jha DK (2012) Plant growth-promoting Rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology 28:1327–1350

    CAS  PubMed  Google Scholar 

  • Bhattacharyya D, Garladinne M, Lee YH (2015) Volatile indole produced by rhizobacterium Proteus vulgaris JBLS202 stimulates growth of Arabidopsis thaliana through auxin, cytokinin, and brassinosteroid pathways. Journal of Plant Growth Regulation 34:158–168

    CAS  Google Scholar 

  • Bric JM, Bostock RM, Silverstone SE (1991) Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. Applied and Environmental Microbiology 57:535–538

    CAS  PubMed  PubMed Central  Google Scholar 

  • Calvo P, Watts DB, Kloepper JW, Torbert HA (2017) Effect of microbial-based inoculants on nutrient concentrations and early root morphology of corn (Zea mays). Journal of Plant Nutrition and Soil Science 180:56–70

    CAS  Google Scholar 

  • Dey R, Pal KK, Bhatt DM, Chauhan SM (2004) Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growth-promoting rhizobacteria. Microbiological Research 159:371–394

    CAS  PubMed  Google Scholar 

  • Dinesh R, Anandaraj M, Kumar A, Srinivasan V, Bini YK, Subila KP, Aravind R, Hamza S (2013) Effects of plant growth promoting rhizobacteria and NPK fertilizers on biochemical and microbial properties of soils under ginger (Zingiber officinale Rosc.) cultivation. Agricultural Research 2:346–353

    CAS  Google Scholar 

  • Dinesh R, Anandaraj M, Kumar A, Srinivasan V, Bini YK, Subila KP, Aravind R (2015) Isolation, characterization, and evaluation of multi-trait plant growth promoting rhizobacteria for their growth promoting and disease suppressing effects on ginger. Microbiological Research 173:34–43

    PubMed  Google Scholar 

  • Garcia FP, Menendez E, Rivas R (2015) Role of bacterial bio fertilizers in agriculture and forestry. AIMS Bioengineering 2:183–205

    Google Scholar 

  • Gómes-Sagasti MT, Marino D (2015) PGPRs and nitrogen-fixing legumes: a perfect team for efficient Cd phytoremediation? Frontiers in Plant Science 6:81

  • Gouda S, Kerry RG, Das G, Paramithiotis S, Shin HS, Patra JK (2018) Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture. Microbiological Research 206:131–140

    PubMed  Google Scholar 

  • Gu Q, Yang Y, Yuan Q, Shi G, Wu L, Lou Z, Huo R, Wu H, Borriss R, Gao X (2017) Bacillomycin D produced by Bacillus amyloliquefaciens is involved in the antagonistic interaction with the plant pathogenic fungus Fusarium graminearum. Applied and Environmental Microbiology 83:e01075-17

  • Hernández-León R, Rojas-Solís D, Contreras-Pérez M, del Carmen Orozco-Mosqueda M, Macías-Rodríguez L, Reyes-de la Cruz H, Valencia-Cantero E, Santoyo G (2015) Characterization of the antifungal and plant growth-promoting effects of diffusible and volatile organic compounds produced by Pseudomonas fluorescens strains. Biological Control 81:83–92

    Google Scholar 

  • Jiang ZQ, Guo YH, Li SM, Qi HY, Guo JH (2006) Evaluation of biocontrol efficiency of different Bacillus preparations and field application methods against Phytophthora blight of bell pepper. Boilogical Control 36:216–223

    Google Scholar 

  • Jiang CH, Liao MJ, Wang HK, Zheng MZ, Xu JJ, Guo JH (2018) Bacillus velezensis, a potential and efficient biocontrol agent in control of pepper gray mold caused by Botrytis cinerea. Boilogical Control 126:147–157

    Google Scholar 

  • Kim HS, Sang MK, Jung HW, Jeun YC, Myung IS, Kim KD (2012) Identification and characterization of Chryseobacterium wanjuense strain KJ9C8 as a biocontrol agent of Phytophthora blight of pepper. Crop Protection 32:129–137

    Google Scholar 

  • Kumar RS, Ayyadurai N, Pandiaraja P, Reddy AV, Venkateswarlu Y, Prakash O, Sakthivel N (2005) Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad-spectrum antifungal activity and biofertilizing traits. Journal of Applied Microbiology 98:145–154

    CAS  PubMed  Google Scholar 

  • Lee KJ, Kamala-Kannan S, Sub HS, Seong CK, Lee GW (2008) Biological control of Phytophthora blight in red pepper (Capsicum annuum L.) using Bacillus subtilis. World Journal of Microbiology and Biotechnology 24:1139–1145

    CAS  Google Scholar 

  • Lee SJ, Park YJ, Kim HT, Kim BS (2010) The race differentiation of Phytophthora capsici in Korea. Research in Plant Disease 16:153–157

    Google Scholar 

  • Li ZJ, Long WP, Zheng JR, Lei JJ (2007) Isolation and identification of Phytophthora capsici in Guangdong province and measurement of their pathogenicity and physiological race differentiation. Journal of South China Agricultural University 28:50–54

    Google Scholar 

  • Liu Y, Pan X, Li J (2015) Current agricultural practices threaten future global food production. Journal of Agricultural and Environmental Ethics 28:203–216

    CAS  Google Scholar 

  • Long HH, Schmidt DD, Baldwin IT (2008) Native bacterial endophytes promote host growth in a species-specific manner; phytohormone manipulations do not result in common growth responses. PLoS One 3:e2702

    PubMed  PubMed Central  Google Scholar 

  • Lugtenberg B, Kamilova F (2009) Plant-growth-promoting rhizobacteria. Annual Review of Microbiology 63:541–556

    CAS  PubMed  Google Scholar 

  • Luna-Bulbarela A, Tinoco-Valencia R, Corzo G, Kazuma K, Konno K, Galindo E, Serrno-Carreón L (2018) Effects of bacillomycin D homologues produced by Bacillus amyloliquefaciens 83 on growth and viability of Colletotrichum gloeosporioides at different physiological stages. Biological Control 127:145–154

    CAS  Google Scholar 

  • Ma HG, He LG, Zhang HL, Li XM, Jiang JX (2013) Physiological races of Phytophthora capsici and their sensitivity to dimethomorph in Jiangxi province. Acta Phytophylacica Sinica 40:374–378

    CAS  Google Scholar 

  • Nautiyal CS (1999) An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters 170:265–270

    CAS  PubMed  Google Scholar 

  • Niu B, Vater J, Rueckert C, Blom J, Lehmann M, Ru JJ, Chen XH, Wang Q, Borriss R (2013) Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1. BMC Microbiology 13:137

    CAS  PubMed  PubMed Central  Google Scholar 

  • Pérez-Flores P, Valencia-Cantero E, Altamirano-Hernández J, Pelagio-Flores R, López-Bucio J, García-Juárez P, Macías-Rodríguez L (2017) Bacillus methylotrophicus M4-96 isolated from maize (Zea mays) rhizoplane increases growth and auxin content in Arabidopsis thaliana via emission of volatiles. Protoplasma 254:2201–2213

    PubMed  Google Scholar 

  • Rybakova D, Cernava T, Köberl M, Liebminger S, Etemadi M, Berg G (2016) Endophytes-assisted biocontrol: novel insights in ecology and the mode of action of Paenibacillus. Plant and Soil 405:125–140

    CAS  Google Scholar 

  • Sang MK, Kim KD (2012) Plant growth-promoting rhizobacteria suppressive to Phytophthora blight affect microbial activities and communities in the rhizosphere of pepper (Capsicum annuum L.) in the field. Applied Soil Ecology 62:88–97

    Google Scholar 

  • Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry 160:47–56

    CAS  PubMed  Google Scholar 

  • Shahbaz M, Ashraf M (2013) Improving salinity tolerance in cereals. Critical Reviews in Plant Sciences 32:237–249

    Google Scholar 

  • Tabassum B, Khan A, Tariq M, Ramzan M, Khan MSI, Shahid N (2017) Bottlenecks in commercialisation and future prospects of PGPR. Applied Soil Ecology 121:102–117

    Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30:2725–2729

    CAS  PubMed  PubMed Central  Google Scholar 

  • Tilman D, Balzer C, Hill J, Befort BL (2011) Global food demand and the sustainable intensification of agriculture. Proceedings of the National Academy of Sciences, USA 108:20260–20264

    CAS  Google Scholar 

  • Vacheron J, Desbrosses G, Bouffaud ML, Touraine B, Moënne-Loccoz Y, Muller D, Legendre L, Wisniewski-Dyé F, Combaret CP (2013) Plant growth promoting rhizobacteria and root system functioning. Frontiers in Plant Science 4:356

    PubMed  PubMed Central  Google Scholar 

  • Weselowski B, Nathoo N, Eastman AW, Macdonald J, Yuan ZC (2016) Isolation, identification and characterization of Paenibacillus polymyxa CR1 with potentials for biopesticide, biofertilization, biomass degradation and biofuel production. BMC Microbiology 16:244

    PubMed  PubMed Central  Google Scholar 

  • Xu SJ, Kim BS (2014) Biocontrol of Fusarium crown and root rot and promotion of growth of tomato by Paenibacillus strains isolated from soil. Mycobiology 42:158–166

    PubMed  PubMed Central  Google Scholar 

  • Xu S, Kim BS (2016) Evaluation of Paenibacillus polymyxa strain SC09-21 for biocontrol of Phytophthora blight and growth stimulation in pepper plants. Tropical Plant Pathology 41:162–168

    Google Scholar 

  • Xu SJ, Hong SJ, Choi W, Kim BS (2014) Antifungal activity of Paenibacillus kribbensis strain T-9 isolated from soils against several plant pathogenic fungi. Plant Pathology Journal 30:102–108

    PubMed  Google Scholar 

  • Yang MY, Cao JF, Li XD, Sun DW, Wang YC, Zhao ZJ (2009) Molecular diagnosis and characterization of blight disease pathogen on pepper in Yunnan. Acta Phytopathologica Sinica 39:297–303

    Google Scholar 

  • Yaoyao E, Yuan J, Yang F, Wang L, Ma J, Li J, Pu X, Raza W, Huang Q, Shen Q (2017) PGPR strain Paenibacillus polymyxa SQR-21 potentially benefits watermelon growth by re-shaping root protein expression. AMB Express 7:104

    Google Scholar 

  • Zhang F, Li XL, Zhu SJ, Ojaghian MR, Zhang JZ (2018) Biocontrol potential of Paenibacillus polymyxa against Verticillium dahlia infecting cotton plants. Biological Control 127:70–77

    Google Scholar 

Download references

Acknowledgments

This work was supported by National Key Research and Development Program of China (2017YFD0201100, 2017YFD0200600), Agricultural Science and Technology Innovation Special Project of Gansu Academy of Agricultural Sciences (2016GAAS26, 2017GAAS23, 2019GAAS02), Agricultural Biotechnology Research and Application Development Project in Gansu Province (GNSW-2016-18).

Author information

Authors and Affiliations

  1. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, China

    Sheng-Jun Xu, Zhuo-Qiong Jing, Zhi-Jie Guo, Qing-Qing Li & Xin-Rui Zhang

  2. Scientific Observing and Experimental Station of Crop Pests in Tianshui, Ministry of Agriculture, Gangu, 741299, People’s Republic of China

    Sheng-Jun Xu, Zhuo-Qiong Jing, Zhi-Jie Guo, Qing-Qing Li & Xin-Rui Zhang

Authors
  1. Sheng-Jun Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhuo-Qiong Jing
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhi-Jie Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qing-Qing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xin-Rui Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sheng-Jun Xu.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xu, SJ., Jing, ZQ., Guo, ZJ. et al. Growth-promoting and disease-suppressing effects of Paenibacillus polymyxa strain YCP16-23 on pepper (Capsicum annuum) plants. Trop. plant pathol. 45, 415–424 (2020). https://doi.org/10.1007/s40858-020-00360-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40858-020-00360-x

Keywords

Search

Navigation