Skip to main content
SpringerLink
Log in

A novel non-segmented double-stranded RNA virus isolated from the basal fungus Conidiobolus sp.

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Mycoviruses are widely distributed in a variety of fungal species. However, few viruses have been reported in basal fungi. A novel non-segmented dsRNA virus was isolated from the basal fungus Conidiobolus sp. of the phylum Zoopagomycota, which has been named "Conidiobolus non-segmented RNA virus 1" (CNRV1). The complete genome sequence of CNRV1 was determined by dsRNA extraction, next-generation sequencing, and RACE. The genome of CNRV1 dsRNA is 3,092 bp in length and contains two open reading frames (ORFs) predicted to encode a subgenomic protein 1 (sgP1) and a putative RNA-dependent RNA polymerase (RdRp). Multiple sequence alignment and phylogenetic analysis based on RdRp sequences from selected dsRNA viruses showed that CNRV1 shared 31.9% sequence identity with Nigrospora oryzae unassigned RNA virus 1 (NoNRV1) and clustered with NoNRV1 and four other mycoviruses. These viruses are unassigned and distant from members of the family Partitiviridae, although they were previously considered partitivirus-like viruses. Thus, CNRV1 is a novel member of proposed genus "Unirnavirus", and is the first dsRNA sequence reported from a member of the phylum Zoopagomycota. This study extends our knowledge about mycoviruses in basal fungi.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Kotta-Loizou I (2019) Mycoviruses: past, present, and future. Viruses 11:361

    Article  Google Scholar 

  2. Ghabrial SA, Castón JR, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479–480:356–368

    Article  Google Scholar 

  3. Nuss DL (2005) Hypovirulence: mycoviruses at the fungal–plant interface. Nat Rev Microbiol 3:632–642

    Article  CAS  Google Scholar 

  4. Xie J, Jiang D (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68

    Article  CAS  Google Scholar 

  5. Liu S, Xie J, Cheng J, Li B, Chen T, Fu Y, Li G, Wang M, Jin H, Wan H, Jiang D (2016) Fungal DNA virus infects a mycophagous insect and utilizes it as a transmission vector. Proc Natl Acad Sci 113:12803–12808

    Article  CAS  Google Scholar 

  6. Lefkowitz EJ, Dempsey DM, Hendrickson RC, Orton RJ, Siddell SG, Smith DB (2017) Virus taxonomy: the database of the International Committee on Taxonomy of Viruses (ICTV). Nucleic Acids Res 46:D708–D717

    Article  Google Scholar 

  7. Koloniuk I, Hrabáková L, Petrzik K (2015) Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 160:1585–1588

    Article  CAS  Google Scholar 

  8. Sutela S, Poimala A, Vainio EJ (2019) Viruses of fungi and oomycetes in the soil environment. FEMS Microbiol Ecol 95:fiz119

    Article  CAS  Google Scholar 

  9. Nie Y, Yu C-Z, Liu X-Y, Huang B (2012) A new species of Conidiobolus (Ancylistaceae) from Anhui, China. Mycotaxon 120:427–435

    Article  Google Scholar 

  10. Spatafora JW, Chang Y, Benny GL, Lazarus K, Smith ME, Berbee ML, Bonito G, Corradi N, Grigoriev I, Gryganskyi A, James TY, O’Donnell K, Roberson RW, Taylor TN, Uehling J, Vilgalys R, White MM, Stajich JE (2016) A phylum-level phylogenetic classification of zygomycete fungi based on genome-scale data. Mycologia 108:1028–1046

    Article  CAS  Google Scholar 

  11. Nibert ML, Debat HJ, Manny AR, Grigoriev IV, De Fine Licht HH (2019) Mitovirus and mitochondrial coding sequences from basal fungus Entomophthora muscae. Viruses 11:351

    Article  CAS  Google Scholar 

  12. Herrero N, Dueñas E, Quesada-Moraga E, Zabalgogeazcoa I (2012) Prevalence and diversity of viruses in the entomopathogenic fungus Beauveria bassiana. Appl Environ Microbiol 78:8523–8530

    Article  CAS  Google Scholar 

  13. Sasai S, Tamura K, Tojo M, Herrero M-L, Hoshino T, Ohki ST, Mochizuki T (2018) A novel non-segmented double-stranded RNA virus from an Arctic isolate of Pythium polare. Virology 522:234–243

    Article  CAS  Google Scholar 

  14. Katoh K, Rozewicki J, Yamada KD (2017) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform 4:1–7

    Google Scholar 

  15. Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549

    Article  CAS  Google Scholar 

  16. Venkataraman S, Prasad BVLS, Selvarajan R (2018) RNA dependent RNA polymerases: insights from structure, function and evolution. Viruses 10:76

    Article  Google Scholar 

  17. te Velthuis AJW (2014) Common and unique features of viral RNA-dependent polymerases. Cell Mol Life Sci 71:4403–4420

    Article  Google Scholar 

  18. Zhou Q, Zhong J, Hu Y, Da Gao B (2016) A novel nonsegmented double-stranded RNA mycovirus identified in the phytopathogenic fungus Nigrospora oryzae shows similarity to partitivirus-like viruses. Arch Virol 161:229–232

    Article  CAS  Google Scholar 

  19. Herrero N (2016) A novel monopartite dsRNA virus isolated from the entomopathogenic and nematophagous fungus Purpureocillium lilacinum. Arch Virol 161:3375–3384

    Article  CAS  Google Scholar 

  20. Kotta-Loizou I, Sipkova J, Coutts RHA (2015) Identification and sequence determination of a novel double-stranded RNA mycovirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 160:873–875

    Article  CAS  Google Scholar 

  21. Zhang T, Jiang Y, Dong W (2014) A novel monopartite dsRNA virus isolated from the phytopathogenic fungus Ustilaginoidea virens and ancestrally related to a mitochondria-associated dsRNA in the green alga Bryopsis. Virology 462–463:227–235

    Article  Google Scholar 

  22. Zhang T, Zeng X, Zeng Z, Hu Z, Yang F (2018) A novel monopartite dsRNA virus isolated from the phytopathogenic fungus Ustilaginoidea virens strain GZ-2. Arch Virol 163:3427–3431

    Article  CAS  Google Scholar 

  23. Shi M, Neville P, Nicholson J, Eden J-S, Imrie A, Holmes EC (2017) High-resolution metatranscriptomics reveals the ecological dynamics of mosquito-associated RNA viruses in Western Australia. J Virol 91:e00680–00617

    Google Scholar 

Download references

Funding

This work was supported by the National Natural Science Foundation of China (grant nos. 31772226, 31471821 and 30770008).

Author information

Author notes

  1. Guogen Yang and Fei Hu contributed equally to this study and share first authorship.

Authors and Affiliations

  1. Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China

    Najie Shi, Ping Wang & Bo Huang

  2. Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China

    Guogen Yang

  3. Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei, 230031, China

    Fei Hu

Authors
  1. Guogen Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fei Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Najie Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ping Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bo Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bo Huang.

Ethics declarations

Conflict of interest

The authors have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling Editor: Ioly Kotta-Loizou.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 12 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, G., Hu, F., Shi, N. et al. A novel non-segmented double-stranded RNA virus isolated from the basal fungus Conidiobolus sp.. Arch Virol 165, 1919–1923 (2020). https://doi.org/10.1007/s00705-020-04692-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-020-04692-6

Search

Navigation