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Molecular profiling of blast resistance genes and evaluation of leaf and neck blast disease reaction in rice

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Abstract

In the present investigation, phenotypic evaluation of blast disease reaction was conducted at Ponnampet and Mandya districts of Karnataka, India, which indicated that the rice varieties such as IR64, Jaya, KMP153, IR30864, Mandya Sona-1, Mandya Sona-2, KCP-1, Dodda Byra, and Malgudi Sanna were susceptible to both leaf and neck blasts. Further, the rice varieties that were resistant to leaf blast such as KMP200, DHMAS70Q164-1b, Karibatta, Coimbatore Sanna and others showed susceptible reaction to neck blast only. In contrast, the varieties such as JyothixBR2655, Punkutt Kodi, Sirsi, 222 and Gangadale which were resistant to neck blast were found to be susceptible to leaf blast also. Only one variety, BR2655 showed resistance to both leaf and neck blast diseases. The genotypic studies using simple sequence repeat markers showed that the analysis of the distribution of resistance genes and genotyping of the selected rice varieties, and traditional rice varieties from different ecological regions with allele specific markers helped to identify 20 major blast resistance genes. The individual gene frequencies of the 20 major rice blast resistance genes varied from 10.34 to 100%. Less and more frequency of resistance gene distribution occurred in Pi9 and Pizt gene, respectively. The result of this study would help to create strategies for improving rice blast resistance through genetic studies and plant‒pathogen interaction.

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Acknowledgements

This project was funded by the Varietal Development Project (Ab. Ac. No. 6709), Government of Karnataka and University of Agricultural Sciences, Bengaluru, India. Thanks to AICRP on Rice faculty at Zonal Agricultural Research Station at Mandya, for their kind support for the project and the traditional rice materials were conserved by Dr M. P. Rajanna, Senior Rice Breeder and Dr C. A. Deepak, Junior Rice Breeder, ZARS, VC Farm, Mandya.

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Author notes

  1. Chikkaballi A. Deepak, Kodihally M. Harinikumar and M. P. Rajanna contributed equally to this work.

Authors and Affiliations

  1. Department of Plant Biotechnology, University of Agricultural Sciences, Bengaluru, 560 065, India

    Haniyambadi B. Manojkumar & Kodihally M. Harinikumar

  2. Zonal Agriculture Research Station, V. C. Farm, Mandya, 571 410, India

    Chikkaballi A. Deepak, M. P. Rajanna & Belthur Chethana

Authors
  1. Haniyambadi B. Manojkumar
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  2. Chikkaballi A. Deepak
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  3. Kodihally M. Harinikumar
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  4. M. P. Rajanna
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  5. Belthur Chethana
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Corresponding author

Correspondence to Haniyambadi B. Manojkumar.

Additional information

Corresponding editor: H. A. Ranganath

HBM: performed all the molecular work such as molecular analysis using trait specific markers and molecular diversity estimation. CAD: planned out the experimental design and assisted to draft manuscript preparation. KMH: associated with the survey of polymorphism among the rice varieties and traditional rice varieties. MPR: participated and planned the plot design at Ponnampet location. BSC: helped in all pathological related work performed in this work. All authors have read and approved the final manuscript.

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Manojkumar, H.B., Deepak, C.A., Harinikumar, K.M. et al. Molecular profiling of blast resistance genes and evaluation of leaf and neck blast disease reaction in rice. J Genet 99, 52 (2020). https://doi.org/10.1007/s12041-020-01212-y

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