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Functional characterization of a receptor for activated C kinase 1 (RACK1) gene from upland cotton (Gossypium hirsutum L.)

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Abstract

Receptor for activated C kinase 1 (RACK1) is a conserved scaffold protein interacting with multiple proteins and participates in diverse cellular activities. Previous high-throughput transcriptome and proteome studies both identified RACK1 gene/protein was differentially expressed in cotton, however, the detailed function of RACK1 in this important fiber-producing plant is still unclear. In the current study, we found that GhRACK1 was preferentially expressed in fast growing tissues, such as cotton fibers and ovules. Down-regulation of GhRACK1 expression in cotton plants through virus-induced gene silencing (VIGS) and ectopic expression of GhRACK1 in Arabidopsis both revealed a positive correlation between GhRACK1 expression level and plant growth rate. Physiological index (protein and soluble sugar content) further indicated that down-regulation of GhRACK1 expression could inhibit protein synthesis and photoassimilate accumulation in cotton, whereas ectopic expression of GhRACK1 exhibited opposite effects in Arabidopsis. These data collectively suggested that GhRACK1 might play an important role in regulating the growth and development of cotton plants.

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Acknowledgements

We are grateful to Professor Jin-Yuan Liu at Tsinghua University for providing constructive comments on the experiments and manuscript.

Funding

This work was supported by Grants from the China Postdoctoral Science Foundation (2014M550074) and State Key Laboratory of Cotton Biology Open Fund (CB2015A01).

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  1. Fangzhi Shu and Bingsheng Wang have contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China

    Fangzhi Shu, Bingsheng Wang, Dalin Liu, Xiaoshan Wang & Bing Zhang

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  1. Fangzhi Shu
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  2. Bingsheng Wang
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  3. Dalin Liu
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  4. Xiaoshan Wang
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Contributions

Zhang B designed the experiments and wrote the manuscript. Shu FZ and Wang BS conducted the experiments and analyzed the data. Liu DL and Wang XS participated in the experiments and help to write the manuscript. All authors read and approved the final manuscript.

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Correspondence to Bing Zhang.

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Table S1. Primers used in this study. Supplementary Material 1. (XLSX 10 kb)

10725_2020_610_MOESM2_ESM.xlsx

Table S2. Information on the six RACK1-like genes in upland cotton (Gossypium hirsutum L.). Supplementary Material 2. (XLSX 10 kb)

Figure S1. SDS-PAGE showing the purification of His-tag GhRACK1 protein. Supplementary Material 3. (TIF 4240 kb)

Figure S2. Diagram showing the location of 300-bp gene fragment (red) for VIGS. Supplementary Material 4. (TIF 990 kb)

10725_2020_610_MOESM5_ESM.tif

Figure S3. Phylogenetic relationship (A), gene structure (B) and protein domain (C) of six RACK1-like genes from upland cotton and three RACK1 genes from Arabidopsis. Supplementary Material 5. (TIF 2800 kb)

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Shu, F., Wang, B., Liu, D. et al. Functional characterization of a receptor for activated C kinase 1 (RACK1) gene from upland cotton (Gossypium hirsutum L.). Plant Growth Regul 91, 359–369 (2020). https://doi.org/10.1007/s10725-020-00610-7

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