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A novel totivirus isolated from the phytopathogenic fungus Rhodosporidiobolus odoratus strain GZ2017

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Abstract

To our knowledge, there have been no reports of mycoviruses infecting Rhodosporidiobolus odoratus. Here, we describe the sequence of a novel mycovirus isolated from R. odoratus, which was designated "Rhodosporidiobolus odoratus RNA virus 1" (RoRV1). Sequence analysis revealed that RoRV1 has two discontinuous open reading frames (ORFs), ORF1 and ORF2, potentially encoding a hypothetical protein and an RNA-dependent RNA polymerase (RdRp), respectively. Phylogenetic analysis based on RdRp sequences clearly placed RoRV1 in the genus Totivirus, family Totiviridae. The fungus also contains two additional, smaller dsRNAs, which might represent RoRV1 satellite RNAs.

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Funding

This study was funded by grants from the National Natural Science Foundation of China (31760015), the Science and Technology Foundation of Guizhou Province ([2017]1153), the Bureau of Science and Technology of Guiyang ([2017]5-24), the Engineering Research Center of Medical Biotechnology, Guizhou Medical University (2016002), and the Immune Cells and Antibody Engineering Research Center of Guizhou Province (KY-2017-017).

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Author notes

  1. Tingting Zhang and Xiaoyao Cai contributed equally to this work.

Authors and Affiliations

  1. Engineering Research Center of Medical Biotechnology/Immune Cells and Antibody Engineering Research Center of Guizhou Province, Guizhou Medical University, Guiyang, 550000, Guizhou Province, People’s Republic of China

    Tingting Zhang, Xiaoyao Cai, Zhu Zeng & Hongmei Liu

  2. School of Biology & Engineering, Guizhou Medical University, Guiyang, 550000, Guizhou Province, People’s Republic of China

    Tingting Zhang, Xiaoyao Cai, Zhu Zeng & Hongmei Liu

  3. School of Basic Medicine, Guizhou Medical University, Guiyang, 550000, Guizhou Province, People’s Republic of China

    Zhu Zeng

  4. The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, 550000, Guizhou Province, People’s Republic of China

    Tingting Zhang & Zhu Zeng

  5. Clinical Research Center, Affiliated Hospital of Guizhou Medical University, Guiyang, 550000, Guizhou Province, People’s Republic of China

    Hang Du

Authors
  1. Tingting Zhang
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  2. Xiaoyao Cai
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  3. Zhu Zeng
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  4. Hang Du
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  5. Hongmei Liu
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Corresponding authors

Correspondence to Tingting Zhang or Hongmei Liu.

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Zhang, T., Cai, X., Zeng, Z. et al. A novel totivirus isolated from the phytopathogenic fungus Rhodosporidiobolus odoratus strain GZ2017. Arch Virol 165, 1911–1914 (2020). https://doi.org/10.1007/s00705-020-04684-6

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  • DOI: https://doi.org/10.1007/s00705-020-04684-6

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