Abstract
Nucleosides are active components in Ophiocordyceps sinensis. All individual nucleoside contents in both fruiting and caterpillar bodies were significantly different among the 24 O. sinensis populations that are distributed across the Qinghai–Tibet Plateau of China. The nucleoside contents of the fruiting and caterpillar bodies show significant positive correlations with the geographical distances of O. sinensis populations, and the nucleoside contents in the O. sinensis populations with the non-dominant fungus–host haplotype associations from isolated geographical areas show significant positive correlations with the genetic distances among those populations based on nrDNA ITS and COI sequences, respectively. The fungus–host associations play important roles in the nucleoside differences of O. sinensis populations.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (81673526). We thank Lesley Benyon, PhD, from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.
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203_2020_1919_MOESM6_ESM.jpg
Fig. S1 HPLC chromatograms for five standard nucleosides and O. sinensis complex samples. Peak 1: cytidine, 2: uridine, 3: inosine, 4: guanosine, and 5: adenosine. A: HPLC chromatograms of the mixture of the five standard nucleosides. B: HPLC chromatogram of the nucleosides of a caterpillar body sampled from population LZBJ (NyingchiBujiu, Tibet) of Tibet. C: HPLC chromatogram of the nucleosides of a fruiting body sampled from population LZBJ (NyingchiBujiu, Tibet) of Tibet. Population codes are defined in Table S1. An Elite C18 column (PerkinElmer Management (Shanghai) Company Limited, Shanghai, China) (250 mm × 4.6 mm i.d., 5 μm) was used. The flow rate was 0.5 ml/min, and the injection volume was 10 µl. The analytes were monitored at 260 nm. Solvents that constituted the mobile phase were (A) methanol and (B) Milli-Q water. The separation was achieved using a 40-min gradient elution. The elution gradient was as follows: 5 min: 5% (A), 10 min: 5% (A), 20 min: 20% (A), 35 min: 15% (A), and 40 min: 5% (A), using a flow rate of 500 μl/min (jpg 28 kb)
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Xu, Z., Li, S., Chen, L. et al. Effects of fungus–host associations on nucleoside differences among Ophiocordyceps sinensis populations on the Qinghai–Tibet Plateau of China. Arch Microbiol 202, 2323–2328 (2020). https://doi.org/10.1007/s00203-020-01919-7
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DOI: https://doi.org/10.1007/s00203-020-01919-7