Abstract
Supercritical fluid encapsulation of doxycycline into aliphatic polyesters was used to fabricate bioresorbable antibacterial polylactoglycolide scaffolds. The distributions of doxycycline concentration on the surface and in the bulk of polymer structures were analyzed using high (≈1 μm) spatial resolution Raman spectroscopy. The release kinetics of doxycycline from these scaffolds into saline solution was studied. It is shown that, with the exception of the first 3 h, when about 15% of its total amount goes into the solution, the release of doxycycline occurs almost linearly. During the first day, the total yield of the antibiotic was about 22%. In 15 days (maximum observation time), the total amount of doxycycline released from polylactoglycolide scaffolds was about 70%. Thus, bioresorbable polymer scaffolds fabricated with supercritical carbon dioxide can be used for local sustained release of antibiotics, as well as for biologically active scaffolds for tissue engineering.
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REFERENCES
Vert, M., Degradable and bioresorbable polymers in surgery and in pharmacology: beliefs and facts, J. Mater. Sci.: Mater. Med., 2009, vol. 20, pp. 437–446.
Martın del Valle, E.M., Galan, M.A., and Carbonell, R.G., Drug delivery technologies: the way forward in the new decade, Ind. Eng. Chem. Res., 2009, vol. 48, pp. 2475–2486.
Mundargi, R.C., Srirangarajan, S., Agnihotri, S.A., et al., Development and evaluation of novel biodegradable microspheres based on poly(d,l-lactide-co-glycolide) and poly (ε-caprolactone) for controlled delivery of doxycycline in the treatment of human periodontal pocket: In vitro and in vivo studies, J. Control. Release, 2007, vol. 119, pp. 59–68.
Roberts, J.A. and Lipman, J., Antibacterial dosing in intensive care: pharmacokinetics, degree of disease and pharmacodynamics of sepsis, Clin. Pharmacokinet., 2006, vol. 45, pp. 755–773.
Hill, J., Klenerman, L., Trustey, S., and Blowers, R., Diffusion of antibiotics from acrylic bone–cement in vitro, J. Bone Jt. Surg., Br. Vol., 1977, vol. 59, no. 2, pp. 197–199.
Jayakumar, P., Deepalakshmi, P., and Shankarram, V., Local drug delivery using doxycycline hyclate (microplast) in the treatment of periodontal disease—a clinical and microbiological evaluation, Int. J. Contemp. Dent., 2011, vol. 2, no. 6, pp. 30–36.
Raval, J.P., Naik, D.R., Amin, K.A., and Patel, P.S., Controlled release and antibacterial studies of doxycycline-loaded poly(ε-caprolactone) microspheres, J. Saudi Chem. Soc., 2014, vol. 18, pp. 566–573.
Kumar, A. and Pilla, J., Implantable drug delivery systems: an overview, in Nanostructures for the Engineering of Cells, Tissues and Organs, Grumezescu, A.M., Ed., Amsterdam: Elsevier, 2018, pp. 473–511.
Nelson, M.L. and Levy, S.B., The history of the tetracyclines, Ann. N.Y. Acad. Sci., 2011, vol. 1241, pp. 17–32.
Popov, V.K., Krasnov, A.P., Volozhin, A.I., and Khoudl, S.M., New bioactive composites for bone tissue regeneration, Perspekt. Mater., 2004, no. 4, pp. 49–57.
Tai, H., Popov, V.K., Shakesheff, K.M., and Howdle, S.M., Putting the fizz into chemistry: applications of delivery and synthesis of novel block copolymers, Biochem. Soc. Trans., 2007, vol. 35, pp. 516–521.
Gumerov, F.M., Sabirzyanov, A.N., and Gumerova, G.I., Sub- i sverkhkriticheskiye flyuidy v protsessakh pererabotki polimerov (Sub- and Supercritical Fluids in Polymer Processing), Kazan: Fen, 2000.
Bogorodskii, S.E., Krotova, L.I., Mironov, A.V., and Popov, V.K., Fabrication of highly porous bioresorbable polymer matrices using supercritical carbon dioxide, Russ. J. Phys. Chem. B, 2013, vol. 7, no. 8, pp. 916–923.
Howdle, S.M., Watson, M.S., Whitaker, M.J., Popov, V.K., et al., Supercritical fluid mixing: preparation of thermally sensitive polymer composites containing bioactive materials, Chem. Commun., 2001, pp. 109–110.
Kapoor, D.N., Bhatia, A., Kaur, R., Sharma, R., Kaur, G., and Dhawan, S., PLGA: a unique polymer for drug delivery, Ther. Delivery, 2015, vol. 6, no. 1, pp. 41–58.
Antonov, E.N., Dunaev, A.G., Minaeva, S.A., Krotova, L.I., and Popov, V.K., Effect of pharmaceutical preparations on the rate of degradation of poly(lactidecoglycolide) scaffolds, Pharm. Chem. J., 2018, vol. 52, no. 1, pp. 69–76.
Funding
This work was supported by the RF Ministry of Education and Science in terms of the Government assignment of the Federal Scientific Research Centre Crystallography and Photonics, Russian Academy of Sciences (development of SCF methods of encapsulation of bioactive substances), and the Russian Foundation for Basic Research (project no. 18-29-06062 mk) (creation of scaffolds and study of release of antibiotics).
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Antonov, E.N., Krotova, L.I., Minaeva, S.A. et al. Release Kinetics of Doxycycline Encapsulated in Polylactoglycolide Scaffolds Using Supercritical Carbon Dioxide. Inorg. Mater. Appl. Res. 11, 366–370 (2020). https://doi.org/10.1134/S2075113320020021
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DOI: https://doi.org/10.1134/S2075113320020021