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Applying the Method of Fluorescence Spectroscopy to Study Dissolved Organic Matter in Waters of the Moskva River

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Moscow University Soil Science Bulletin Aims and scope

Abstract

The applicability of fluorescence spectroscopy for studying the dissolved organic matter in the Moskva River has been shown. The most typical surface water fluorophores—humic and fulvic acids and protein substances—have been revealed in the studied water samples. The fluorescence intensity depends on the sampling site and indicates the contamination rate. The dynamics of the composition and fluorescence parameters of river waters varies with respect to the sampling period. While the concentration of dissolved organic matter and chemical oxygen demand increases from October until November, specific ultraviolet absorbance, biological oxygen demand, and fluorescence of humic acids significantly decrease. The observed dynamics does not depend on the sampling site, which confirms the effect of climatic conditions. Statistically significant (p < 0.05) correlations between spectral and chemical parameters of water contamination have been revealed. Fluorescence intensity, tryptophan-containing organic substances, fulvic acids, and values of biological index correlate (r = 0.63–0.92) with the content of ammonium and phosphate ions. The fluorescent index A may be used to determine the zone of the effect of anthropogenic biological impurities on the status of waters of the Moskva River.

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REFERENCES

  1. Andrianova, M.Yu., The way to check-up bioorganic additions in surface water source and drinking water supply system by means of spectrofluorometry, Extended Abstract of Cand. Sci. (Eng.) Dissertation, St. Petersburg, 2008.

  2. SanPiN (Sanitary Regulations) 2.1.5.980-00: Hygienic Norms for Surface Water Protection, Moscow, 2001.

  3. Gladilovich, D.B., Fluorometric method for checking-up oil products content in water, Partnery Konkurenty, 2001, no. 12.

  4. Gorshkova, O., Patsaeva, S., Fedoseeva, S., et al., Fluorescence of dissolved organic matter in natural water, Voda: Khim. Ekol., 2009, no. 11.

  5. Weather diary for Moscow. https://www.gismeteo.ru/diary/4368/2014/11/. Accessed August 20, 2018.

  6. von Parker, C.A., Photoluminescence of Solutions, Amsterdam: Elsevier, 1968.

    Google Scholar 

  7. GN (Sanitary-Hygienic Standard) 2.1.5.1315-03: Maximum Permissible Concentrations of Chemical Matters in Drinking and Social Water Reservoirs, Moscow, 2003.

  8. Pushkar’, V.Ya., Shchegol’kova, N.M., and Kozlov, M.N., Biotests for biologically purified waste water, Ekol. Prom. Ross., 2006, no. 4.

  9. Uchevatkina, N.V., Nefedkin, S.I., Zav’yalova, A.A., et al., The way to analyze Moscow river pollution by ammonium nitrogen in Moscow Region and ways for lowering effect of nitrogen-content compounds onto water objects, Ekol. Prom. Proizvod., 2005, no. 4.

  10. Baker, A., Fluorescence properties of some farm wastes: implications for water quality monitoring, Water Res., 2002, vol. 36, no. 1, pp. 189–195.

    Article  Google Scholar 

  11. Baker, A. and Inverarity, R., Protein-like fluorescence intensity as a possible tool for determining river water quality, Hydrol. Proc., 2004, vol. 18, no. 15, pp. 2927–2945.

    Article  Google Scholar 

  12. Baker, A., Inverarity, R., Charlton, M., and Richmond, S., Detecting river pollution using fluorescence spectrophotometry: case studies from the Ouseburn, NE England, Environ. Pollut., 2003, vol. 124, no. 1, pp. 57–70.

    Article  Google Scholar 

  13. Baker, A. and Spencer, R., Characterization of DOM from source to sea using fluorescence and absorbance spectroscopy, Sci. Total Environ., 2004, vol. 333, no. 1–3, pp. 217–232.

    Article  Google Scholar 

  14. Birdwell, J.E. and Engel, A.S., Variability in terrestrial and microbial contributions to dissolved organic matter fluorescence in the Edwards Aquifer, Central Texas, J. Cave Karst Stud., 2009, vol. 71, pp. 144–156.

    Google Scholar 

  15. Carstea, E., Fluorescence spectroscopy as a potential tool for in-situ monitoring of dissolved organic matter in surface water systems, InTech, 2012. http://www.intechopen.com/books/water-pollution/fluorescence-spectroscopy-as-a potential-tool-for-in-situ-monitoring-of-dissolved-organic-matter-in. Cited 15.06.2018.

  16. Chen, J., LeBoeuf, E.J., Dai, S., et al., Fluorescence spectroscopic studies of natural organic matter fractions, Chemosphere, 2003, vol. 50, no. 5.

    Article  Google Scholar 

  17. Christ, M. and David, M., Temperature and moisture effects on the production of dissolved organic carbon in a spodosol, Soil Biol. Biochem., 1996, vol. 28, no. 9, pp. 1191–1199.

    Article  Google Scholar 

  18. Corvasce, M., Zsolnay, A., D’Orazio, V., et al., Characterization of water extractable organic matter in a deep soil profile, Chemosphere, 2006, vol. 62, no. 10, pp. 1583–1590.

    Article  Google Scholar 

  19. Ghervase, L., Carstea, E., Pavelescu, G., et al., Laser induced fluorescence efficiency in water quality assessment, Roman. Rep. Phys., 2010, vol. 62, no. 3, pp. 652–659.

    Google Scholar 

  20. Goldman, J., Rounds, S., and Needoba, J., Applications of fluorescence spectroscopy for predicting percent wastewater in an urban stream, Environ. Sci. Technol., 2012, vol. 46, no. 8, pp. 4374–4381.

    Article  Google Scholar 

  21. He, X.-S., Xi, B.-D., Wei, Z.-M., et al., Fluorescence EEM spectroscopy with regional integration analysis for characterizing composition and transformation of DOM in landfill leachates, J. Hazard. Mater., 2011, vol. 190, nos. 1–3, pp. 293–299.

    Article  Google Scholar 

  22. Henderson, R., Baker, A., Murphy, K., et al., Fluorescence as a potential monitoring tool for recycled water systems: a review, Water Res., 2009, vol. 43, no. 4, pp. 863–881.

    Article  Google Scholar 

  23. Hudson, N., Baker, A., and Reynolds, D., Fluorescence analysis of dissolved organic matter in natural, waste and polluted waters: a review, River Res. Appl., 2007, vol. 23, no. 6.

    Article  Google Scholar 

  24. Hudson, N., Baker, A., Ward, D., et al., Can fluorescence spectrometry be used as a surrogate for the biochemical oxygen demand test in water quality assessment? An example from South West England, Sci. Total Environ., 2008, vol. 391, no. 1, pp. 149–158.

    Article  Google Scholar 

  25. Huguet, A., Vacher, L., Relexans, S., et al., Properties of fluorescent dissolved organic matter in the Gironde Estuary, Org. Geochem., 2009, vol. 40, no. 6, pp. 706–719.

    Article  Google Scholar 

  26. Jaffrain, J. and Gurard, F., Assessing the quality of DOM in forest soils using ultraviolet absorption spectrophotometry, Soil Sci. Soc. Am. J., 2007, vol. 71, no. 6.

  27. McKnight, D.M., Boyer, E.W., and Westerhoff, P.K., Spectrofluorometric characterization of dissolved organic matter for indication of precursor organic material and aromaticity, Limnol. Oceanogr., 2001, vol. 46, no. 1, pp. 38–48.

    Article  Google Scholar 

  28. Ohno, T., Fluorescence inner-filtering correction for determining the humification index of dissolved organic matter, Environ. Sci. Technol., 2002, vol. 36, no. 4, pp. 742–746.

    Article  Google Scholar 

  29. Para, J., Coble, P., Charrière, B., et al., Fluorescence and absorption properties of chromophoric dissolved organic matter in coastal surface waters of the Northwestern Mediterranean Sea, influence of the Rhone River, Biogeoscience, 2010, vol. 7, pp. 4083–4103.

    Article  Google Scholar 

  30. Parlanti, E., Worz, K., Geoffroy, L., and Lamotte, M., Dissolved organic matter fluorescence spectroscopy as a tool to estimate biological activity in a coastal zone submitted to anthropogenic inputs, Org. Geochem., 2000, vol. 31, no. 12, pp. 1765–1781.

    Article  Google Scholar 

  31. Roth, V.-N., Dittmar, T., Gaupp, R., and Gleixner, G., The molecular composition of dissolved organic matter in forest soils as a function of pH and temperature, PLoS One, 2015, vol. 10, p. e0119188. https://doi.org/10.1371/journal.pone.0119188

    Article  Google Scholar 

  32. Świetlik, J. and Sikorska, E., Characterization of NOM fractions by HPSECh, specific UV absorbance and total luminescence spectroscopy, Polish J. Environ. Stud., 2005, vol. 15, no. 1.

  33. Wang, H., Holden, J., Zhang, Z., et al., Concentration dynamics and biodegradability of dissolved organic matter in wetland soils subjected to experimental warming, Sci. Total Environ., 2014, vol. 470–471, pp. 907–916.

    Article  Google Scholar 

  34. Zsolnay, A., Baigar, E., Jimenez, M., et al., Differentiating with fluorescence spectroscopy the sources of DOM in soils subjected to drying, Chemosphere, 1999, vol. 38, no. 1, pp. 45–50.

    Article  Google Scholar 

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Correspondence to E. I. Karavanova.

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Translated by I. Bel’chenko

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Karavanova, E.I., Konovalov, A.G. & Terskaya, E.V. Applying the Method of Fluorescence Spectroscopy to Study Dissolved Organic Matter in Waters of the Moskva River. Moscow Univ. Soil Sci. Bull. 74, 199–207 (2019). https://doi.org/10.3103/S014768741905003X

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  • DOI: https://doi.org/10.3103/S014768741905003X

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