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Pluronics and Brij-35 Reduce the Bacteriolytic Activity of Lysozyme

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Abstract

The influence of nonionic surfactants on the bacteriolytic activity of lysozyme is studied on the model cells of Micrococcus luteus using Pluronics P123, L121, and F127, as well as Brij-35. These compounds form complexes with lysozyme through hydrogen-bonding interactions between the ethylene oxide fragments of surfactant and the amino acids residues of lysozyme surface. The bacteriolytic activity of lysozyme decreases in the order of F127 < P123 < L121 ≈ Brij-35, probably because of the steric hindrances of the protein’s interaction with the cells’ substrate.

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Funding

The work was supported by the Russian Foundation for Basic Research (project no. 18-33-20147-mol-a-ved).

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Authors and Affiliations

  1. Department of Chemistry, Divisions of Radiochemistry, Moscow State University, Moscow, Russia

    A. V. Shnitko, M. G. Chernysheva & G. A. Badun

  2. Department of Chemistry, Divisions of Chemical Enzymology, Moscow State University, Moscow, Russia

    S. A. Smirnov & P. A. Levashov

Authors
  1. A. V. Shnitko
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  2. M. G. Chernysheva
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  3. S. A. Smirnov
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  4. P. A. Levashov
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  5. G. A. Badun
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Correspondence to A. V. Shnitko.

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Translated by E. Makeeva

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Shnitko, A.V., Chernysheva, M.G., Smirnov, S.A. et al. Pluronics and Brij-35 Reduce the Bacteriolytic Activity of Lysozyme. Moscow Univ. Chem. Bull. 75, 92–95 (2020). https://doi.org/10.3103/S0027131420020145

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  • DOI: https://doi.org/10.3103/S0027131420020145

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