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Testing a Novel Heat-Shock Protein Inducer in the Cellular Model of Traumatic Brain Injury Response

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Abstract—

Traumatic brain injury (TBI) induces multiple pathological processes affecting various brain cells. the accumulation of toxic factors in interstitial and cerebrospinal fluid may be a consequence of TBI and massive cell death. The accumulation process, causing so-called “secondary damage,” is not transient and often lasts within days and weeks. We believe that Hsp70 protein can play an important role in reducing the severity of posttraumatic pathological complications. Chaperone Hsp70 is known for its cytoprotective activity, and, therefore, an approach involving its increase in in cells exposed to proapoptotic and proinflammatory factors may turn out to be extremely promising. In support of this idea, we studied the effect of the low molecular weight substance KD-29, which is able to induce the synthesis of Hsp70 in the cellular model of the posttraumatic process. We used rat C6 glioma cells to study the cytotoxic effect of rat cerebrospinal fluid obtained after TBI. We found that the drug KD-29 significantly inhibited the apoptosis process and increased the proliferative activity of C6 cells under conditions of modeling posttraumatic processes.

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ACKNOWLEDGMENTS

We are grateful to E.R. Mikhailova (Institute of Cytology, Russian Academy of Sciences) for participation in the screening of chemical compounds from the UFU library.

Funding

This study was supported by the Russian Science Foundation, project no. 18-74-10087.

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Correspondence to V. F. Lazarev.

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Conflict of interest. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. Animal experiments were approved by the Ethics Committee of the Institute of Cytology, Russian Academy of Sciences, and Institute of Health, Bethesda, Maryland, United States, registration no. F18-00380.

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Translated by I. Fridlyanskaya

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Lazarev, V.F., Dutysheva, E.A., Trestsova, M.A. et al. Testing a Novel Heat-Shock Protein Inducer in the Cellular Model of Traumatic Brain Injury Response. Cell Tiss. Biol. 14, 52–56 (2020). https://doi.org/10.1134/S1990519X20010071

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  • DOI: https://doi.org/10.1134/S1990519X20010071

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