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The Role of Endotheliocytes of Liver Sinusoid Capillaries in the Pathogenesis of Disorders in the Vascular-Platelet Link of Hemostasis in the Acute Period of Traumatic Brain Injury

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Abstract—

In rats subjected to traumatic brain injury (TBI), at the stage of acute development of traumatic disease, the platelet number, ultrastructural changes in liver sinusoid hemocapillaries (SCs), and microcirculation disorders in their lumen were studied. The results of the study showed that, in local brain injury, on the third day of post-traumatic period, thrombocytopenia, expressed lesions of liver SCs, and intrasinusoid microcirculation disorders, which are aggravated on the seventh day post injury, are detected. On the 12th day after TBI, the sinusoid endothelium is detected to be of usual appearance, but yet areas of its swelling and edema occur. In 79% of sinusoid hemocapillaries, the blood flow is recovered, while in 21% macro- and micro aggregates of erythrocytes, bubbles, cellular detritus, and platelets are detected. Analyzing the obtained data, we can conclude that the detected lesions of liver SC endotheliocytes in the acute period of TBI make it possible to consider them one of the key, and earliest, links of pathogenesis of the vascular–platelet link of hemostasis.

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Funding

This work was carried out as part of the research work of the Anesthesiology and Intensive Care Department, Additional Professional Education Faculty, Privolzhsky Research Medical University of Ministry of Public Health of Russian Federation, Russia, and supported by the Russian Foundation for Basic Research, project no. 18-34-00831.

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Authors and Affiliations

  1. Anesthesiology and Intensive Care Department, Additional Professional Education Faculty, Privolzhsky Research Medical University of the Ministry of Public Health of the Russian Federation, Nizhny Novgorod, Russia

    G. A. Boyarinov, Ye. I. Yakovleva, O. D. Solovyeva, L. V. Boyarinova, A. V. Polozova, Ye. V. Moshnina & L. A. Shchegolkov

  2. Physiology and Anatomy Department of Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia

    A. V. Deryugina & A. V. Polozova

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  1. G. A. Boyarinov
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  2. Ye. I. Yakovleva
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  4. O. D. Solovyeva
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  5. L. V. Boyarinova
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  8. L. A. Shchegolkov
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Correspondence to G. A. Boyarinov or A. V. Deryugina.

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Statement on the welfare of animals. Animal keeping and manipulations of them were performed according to the regulations presented in the Guide for Care and Use of Laboratory Animals (ILAR publication, 1996, National Academy Press) and the demands of the Order of Ministry of Public Health of Russia no. 267 of June 19, 2003, “On the Establishment of Rules of Laboratory Practice in Russian Federation.”

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Abbreviations: SC—liver sinusoid hemocapillary, TF—tissue factor, TBI—traumatic brain injury.

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Boyarinov, G.A., Yakovleva, Y.I., Deryugina, A.V. et al. The Role of Endotheliocytes of Liver Sinusoid Capillaries in the Pathogenesis of Disorders in the Vascular-Platelet Link of Hemostasis in the Acute Period of Traumatic Brain Injury. Cell Tiss. Biol. 14, 43–51 (2020). https://doi.org/10.1134/S1990519X20010046

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