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Karyotype Characteristics and the Composition of Hemoglobins in Chironomus sp. Ya3 (Diptera, Chironomidae) from the Pechora Delta

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Abstract

The paper reports data on the karyotype structure and hemoglobin composition of Chironomus sp. Ya3 (Kiknadze, Istomina et Salova, 1996) found in a pond in the Pechora River delta within the permafrost zone. The collected larvae morphologically corresponded to the original description. Of the 9 chromosome banding sequences detected in them, Ya3A2 and Ya3E2 were for the first time recorded in the homozygous and heterozygous state, respectively. Electrophoresis data demonstrated a complex hemoglobin structure. Disc electrophoresis revealed 9 fractions with relative mobility Rf from 0.43 to 0.84, formed by subunits with molecular weights from 11.5 to 15.6 kDa. The complex structure of the hemoglobin probably ensures survival of the species under the extreme local environmental conditions. The species resembles Ch. plumosus in the native hemoglobin composition and shows great similarity with Ch. riparius in the subunit composition of individual hemoglobin fractions.

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ACKNOWLEDGMENTS

The authors are grateful to A.S. Glotov and S.A. Zolotoy (Nenets Nature Reserve) for organizing field research in the Pechora delta, to V.K. Chugunov (Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences) for help with primary material processing, and to colleagues from the Chironomidae evolutionary genomics research group (Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences) for valuable comments.

Funding

This work was carried out within the framework of State research assignments AAAA-A18-118012690123-4 and AAAA-A17-117112850235-2 and supported in part by the Integrated research program of the Ural Branch, Russian Academy of Sciences (AAAA-A18-118011390005-9) and the Russian Foundation for Basic Research (project r_a 18-44-110017).

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Authors and Affiliations

  1. Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, 152742, Borok, Russia

    V. V. Bolshakov 

  2. Institute of Biology, Komi Scientific Center, Ural Branch, Russian Academy of Sciences, 167982, Syktyvkar, Russia

    E. B. Fefilova 

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  1. V. V. Bolshakov 
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  2. E. B. Fefilova 
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Correspondence to V. V. Bolshakov .

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The authors declare that they have no conflict of interest. All applicable international, national, and institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Bolshakov , V.V., Fefilova , E.B. Karyotype Characteristics and the Composition of Hemoglobins in Chironomus sp. Ya3 (Diptera, Chironomidae) from the Pechora Delta. Entmol. Rev. 100, 1–6 (2020). https://doi.org/10.1134/S0013873820010017

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