Abstract
The level of cytogenetic damage was analyzed in coal miners (N = 116) and the control sample (N = 169) using an assay for scoring of chromosomal aberrations (CAs) and the DNA comet assay in lymphocytes, as well as the micronucleus test in buccal epithelial cells (MN in BEC). The group of coal miners was characterized by a statistically significantly increase in the main index values of the used test system, compared to the control group. Specifically, the level of chromosomal aberrations in the miner and control groups was 4.69 ± 0.28 and 2.13 ± 0.10%; the proportion of DNA in the comet tail was 4.33 ± 0.38 and 2.16 ± 0.24%; and the micronucleus level was 1.44 ± 0.21 and 0.23 ± 0.12‰, respectively (Р < 0.05). Moreover, in the sample of coal miners, a considerable increase in the levels of additional test indices was observed. For CAs, these parameters included the frequency of single fragments, chromatid-type aberrations, paired fragments, dicentrics without fragments, chromosome exchanges, and chromosome-type aberrations; for the DNA comet assay, these were the comet tail moment and Olive tail moment; for the MN in BEC, the frequencies of binucleated cells, cells with notched nuclei, geminate nuclei, karyorhexis, and apoptotic bodies. Ranking of the results according to major cytogenetic abnormalities made it possible to establish that, in the group of miners, the proportion of individuals with the index values above the background was 68.97% for CAs, 52.13% for the DNA comet assay, and 36.08% for the MN in BEC. The proportion of individuals with cytogenetic damage higher than the background simultaneously in three test systems was 20% of the total sample of miners. To assess the mutagenic effects from occupational factors of coal mining enterprises, it seems reasonable to use a complex of test systems (CA test, DNA comet assay, and MN in BEC).
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REFERENCES
Armutcu, F., Gun, B.D., Altin, R., and Gurel, A., Examination of lung toxicity, oxidant/antioxidant status and effect of erdosteine in rats kept in coal mine ambience, Environ. Toxicol. Pharmacol., 2007, vol. 24, no. 2, pp. 106—113. https://doi.org/10.1016/j.etap.2007.03.002
Leon-Mejia, G., Silva, L.F., Civeira, M.S., et al., Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells, Environ. Sci. Pollut. Res. Int., 2016, vol. 23, no. 23, pp. 24019—24031. https://doi.org/10.1007/s11356-016-7623-z
Collins, A.R., Oscoz, A.A., Brunborg, G., et al., The comet assay: topical issues, Mutagenesis. 2008, vol. 23, pp. 143—151. https://doi.org/10.1093/mutage/gem051
Vinzents, P.S., Moller, P., Sorensen, M., et al., Personal exposure to ultrafine particles and oxidative DNA damage, Environ. Health Perspect. 2005, vol. 113, pp. 1485—1490. https://doi.org/10.1289/ehp.7562
Bonassi, S., Znaor, A., Norppa, H., and Hagmar, L., Chromosomal aberrations and risk of cancer in humans: an epidemiologic perspective, Cytogenet. Genome Res., 2004, vol. 104, nos. 1—4, pp. 376—382. https://doi.org/10.1159/000077519
Sycheva, L.P., Assessment of mutagenic effects of environmental factors using polyorganic micronucleus test, Vestn. Ross. Akad. Med. Nauk, 2006, no. 7, pp. 27—32.
Matzenbacher, C.A., Garcia, A.L.H., Santos, M.S., et al., DNA damage induced by coal dust, fly and bottom ash from coal combustion evaluated using the micronucleus test and comet assay in vitro, J. Hazard. Mater., 2017, vol. 324, pp. 781—788. https://doi.org/10.1016/j.jhazmat.2016.11.062
da Silva Júnior, F., Tavella, R., Fernandes, C., et al., Genotoxicity in Brazilian coal miners and its associated factors, Hum. Exp. Toxicol., 2018, vol. 37, no. 9, pp. 891—900. https://doi.org/10.1177/0960327117745692
Leon-Mejia, G., Espitia-Perez, L., Hoyos-Giraldo, L.S., et al., Assessment of DNA damage in coal open-cast mining workers using the cytokinesis-blocked micro micronucleus test and the comet assay, Sci. Total Environ., 2011, vol. 409, no. 4, pp. 686—691.https://doi.org/10.1016/j.scitotenv.2010.10.049
Donbak, L., Rencuzogullar, E., Yavuz, A., and Topaktas, M., The genotoxic risk of underground coal miners from Turkey, Mutat. Res., 2005, vol. 588, no. 2, pp. 82—87. https://doi.org/10.1016/j.mrgentox.2005.08.014
Rohr, P., Kvitko, K., Silva, F.R., et al., Genetic and oxidative damage of peripheral blood lymphocytes in workers with occupational exposure to coal, Mutat. Res., 2013, vol. 758, nos. 1—2, pp. 23—28. https://doi.org/10.1016/j.mrgentox.2013.08.006
Smerhovsky, Z., Landa, K., Rossner, P., et al., Increased risk of cancer in radon-exposed miners with elevated frequency of chromosomal aberrations, Mutat. Res., 2002, vol. 514, nos. 1—2, pp. 165—176.
Santa Maria, S.R., Arana, M., and Ramirez, O., Chromosomal aberrations in peripheral lymphocytes from male native miners working in the Peruvian Andes, Genet. Mol. Biol., 2007, vol. 30, no. 4, pp. 1135—1138. https://doi.org/10.1590/S1415-47572007000600017
Minina, V.I., Kulemin, Yu.E., Tolochko, T.A., et al., Genotoxic effects of the working environment on the Kuzbass miners, Med. Truda Prom. Ekol., 2015, no. 5, pp. 4—8.
Volobaev, V.P., Larionov, A.V., Kalyuzhnaya, E.E., et al., Associations of polymorphisms in the cytokine genes IL1β (rs16944), IL6 (rs1800795), IL12b (rs3212227) and growth factor VEGFA (rs2010963) with anthracosilicosis in coal miners in Russia and related genotoxic effects, Mutagenesis, 2018, vol. 33, no. 2, pp. 129—135. https://doi.org/10.1093/mutage/gex047
Sinitsky, M.Y., Minina, V.I., Gafarov, N.I., et al., Assessment of DNA damage in underground coal miners using the cytokinesis-block micronucleus assay in peripheral blood lymphocytes, Mutagenesis, 2016, vol. 31, no. 6, pp. 669—675. https://doi.org/10.1093/mutage/gew038
Rohr, P., Silva, J., Silva, F.R., et al., Evaluation of genetic damage in open-cast coal mine workers using the buccal micronucleuscytome assay, Environ. Mol. Mutagen., 2013, vol. 54, no. 1, pp. 65—71. https://doi.org/10.1002/em.21744
Leon-Mejia, G., Quintana, M., Debastiani, R., et al., Genetic damage in coal miners evaluated by buccal micronucleus cytome assay, Ecotoxicol. Environ. Saf., 2014, vol. 107, pp. 133—139. https://doi.org/10.1016/j.ecoenv.2014.05.023
Hungerford, P.A., Leukocytes cultured from small inocula of whole blood and the preparation of metaphase chromosomes by treatment with hypotonic KCl, Stain Techn., 1965, vol. 40, pp. 333—338.
Druzhinin, V.G., Quantitative characteristics of chromosome aberration frequency in the human population of a large Western Siberian industrial region, Russ. J. Genet., 2003, vol. 39, no. 10, pp. 1161—1167. https://doi.org/10.1023/A:1026179011781
Singh, N.P., McCoy, M.T., Tice, R.R., and Schneider, E.L., A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res., 1988, vol. 175, pp. 184—191.
Konca, K., Lankoff, A., Banasik, A., et al., A cross platform public domain PC image analysis program for the comet assay, Mutat. Res., 2003, vol. 534, nos. 1—2, pp. 15—20.
Thomas, P., Hollad, N., Bolognesi, C., et al., Buccal micronucleus cytome assay, Nat. Protoc., 2009, vol. 4, pp. 825—837.
Sycheva, L.P., Biological significance, determination criteria and variation limits of the full range of karyological indicators in assessing human cytogenetic status, Med. Genet., 2007, no. 11, pp. 3—11.
Batar, B., Guven, M., Baris, S., et al., DNA repair gene XPD and XRCC1 polymorphisms and the risk of childhood acute lymphoblastic leukemia, Leuk. Res., 2009, vol. 33, pp. 759—763. https://doi.org/10.1016/j.leukres.2008.11.005
Jiang, J., Zhang, X., Yang, H., and Wang, W., Polymorphisms of DNA repair genes: ADPRT, XRCC1, and XPD and cancer risk in genetic epidemiology, Meth. Mol. Biol., 2009, vol. 471, pp. 305—333. https://doi.org/10.1007/978-1-59745-416-2_16
Makhalin, A.V., Redkokasha, L.Yu., and Moroz, V.V., Cytogenetic changes in T-lymphocytes among miners, Obsh. Reanimatol., 2007, vol. 3, nos. 5—6, pp. 139—143. https://doi.org/10.15360/1813-9779-2007-6-139-143
Meier, A.V., Tolochko, T.A., Litvin, A.V., et al., Karyological status of buccal epithelial cells of miners with occupational pulmonary pathologies, Gig. Sanit., 2018, vol. 97, no. 3, pp. 220—225.
Kvitko, K., Bandinelli, E., Henriques, J.A.P., et al., Susceptibility to DNA damage in workers occupationally exposed to pesticides, to tannery chemicals and to coal dust during mining, Genet. Mol. Biol., 2012, vol. 35, no. 4, pp. 1060—1068.
Cabarcas-Montalvo, M., Olivero-Verbel, J., and Corrales-Aldana, H., Genotoxic effects in blood cells of Mus musculus and Iguana iguana living near coal mining areas in Colombia, Sci. Total Environ., 2012, vol. 416, pp. 208—214. https://doi.org/10.1016/j.scitotenv.2011.11.080
Minina, V.I., Druzhinin, V.G., and Golovina, T.A., Dynamics of chromosomal aberrations level in residents of an industrial city in conditions of changing atmosphere pollution, Ekol. Genet., 2014, vol. 12, no. 3, pp. 60—68. https://doi.org/10.17816/ecogen12360-70
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This study was supported by the Russian Science Foundation (grant no. 18-14-00022).
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Meyer, A.V., Tolochko, T.A., Minina, V.I. et al. Complex Approach to Evaluating Genotoxicity from Occupational Factors in Coal Mining Industry. Russ J Genet 56, 611–617 (2020). https://doi.org/10.1134/S1022795420050105
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DOI: https://doi.org/10.1134/S1022795420050105