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Emulsion PCR Amplification of DNA Libraries with Degenerate Central Regions for Aptamer Selection

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Abstract

For emulsion PCR amplification of oligonucleotide combinatorial libraries the conditions for the stable generation of water-in-oil emulsions with drops of optimal size were found, which included the use of 3% ABIL EM 180 silicone emulsifier in the presence of 0.5% Triton Х100 in mineral oil and controlled mechanical mixing.

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ACKNOWLEDGMENTS

The studies were conducted using the equipment of the Center of Collective Use Biomika and a unique scientific system KODINK.

Funding

The work was supported by the Russian Foundation for Basic Research, project no. 18-29-09151/18.

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

    V. E. Kuznetsova, V. E. Shershov, R. A. Miftahov, S. A. Lapa, A. S. Zasedatelev, E. N. Timofeev & A. V. Chudinov

Authors
  1. V. E. Kuznetsova
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  2. V. E. Shershov
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  3. R. A. Miftahov
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  4. S. A. Lapa
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  5. A. S. Zasedatelev
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  6. E. N. Timofeev
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  7. A. V. Chudinov
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Corresponding author

Correspondence to A. V. Chudinov.

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COMPLIANCE WITH ETHICAL STANDARDS

In this work, humans or animals were not involved as subjects of studies.

Conflict of Interests

The authors notified about the absence of conflict of interest.

Additional information

Translated by E. Shirokova

Abbreviations: ePCR, emulsion polymerase chain reaction; SELEX, systematic evolution of ligands by exponential enrichment.

Corresponding author: phone: +7 (499) 135-9800; fax: +7 (495) 135-1405; e-mail: chud@eimb.ru.

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Kuznetsova, V.E., Shershov, V.E., Miftahov, R.A. et al. Emulsion PCR Amplification of DNA Libraries with Degenerate Central Regions for Aptamer Selection. Russ J Bioorg Chem 46, 264–268 (2020). https://doi.org/10.1134/S1068162020020120

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  • DOI: https://doi.org/10.1134/S1068162020020120

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