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Validated Immunosorbent Surface-Based Determination of Theophylline in Milk

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Abstract

In the present study, an immunosorbent surface-based theophylline determination method was developed and validated. The procedure was tested on milk samples. Theophylline was removed from milk by an immunosorbent surface. The efficiency of theophylline removal was found to be between 95–97%. The proposed method is cost effective and does not require any special equipment. Detection and quantification limits of the proposed method were determined based on the standard deviation of 10 ng/mL theophylline and they were equal 3 and 11 ng/mL, respectively. Results were also validated for precision, accuracy and linearity.

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Funding

We thank to Marmara University Scientific Research Projects Commission for supporting this study (Project number: SAG-B-130511-0122).

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Authors and Affiliations

  1. Marmara University, Faculty of Dentistry, Department of Basic Medical Sciences, Istanbul, Turkey

    Tugba Tunali Akbay, Hazal Ipekci & Aysen Yarat

Authors
  1. Tugba Tunali Akbay
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  2. Hazal Ipekci
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  3. Aysen Yarat
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Correspondence to Tugba Tunali Akbay.

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Tugba Tunali Akbay, Ipekci, H. & Yarat, A. Validated Immunosorbent Surface-Based Determination of Theophylline in Milk. J Anal Chem 75, 640–644 (2020). https://doi.org/10.1134/S1061934820050020

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  • DOI: https://doi.org/10.1134/S1061934820050020

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