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Characterization of transcription activation domain of EcaICE1 and its interaction with EcaSIZ1 in Eucalyptus camaldulensis

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Abstract

SUMOylation in plants is associated with biotic and abiotic stress responses, flowering, and other aspects of development. ICE1 protein SUMOylation by the SUMO E3 ligase SIZ1 plays important roles in plant cold tolerance. Here, we reported the subcellular localization of EcaICE1 and its interaction with EcaSIZ1 in Eucalyptus camaldulensis. The genes EcaICE1 and EcaSIZ1 were isolated by homologous cloning. The subcellular localization analysis showed that EcaICE1 was located in nucleus. Bimolecular fluorescence complementation (BiFC) analysis revealed that EcaICE1 could interact with EcaSIZ1 in the nucleus of Nicotiana benthamiana leaves. Moreover, yeast two-hybrid assay confirmed that the amino acid region from position 84 to 126 in EcaICE1 was critical for the strong transactivation activity of EcaICE1 and that the C-terminal region from position 361 to 557 in EcaICE1 was the key region for its interaction with EcaSIZ1 using different truncated lengths of non-transactivation activity of EcaICE1 as the bait protein. Collectively, our results showed that EcaICE1 may have a SUMOylation pathway similar to Arabidopsis thaliana.

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Acknowledgements

This work has been supported by NSFC Project 31470673. We also wish to express our appreciation to the anonymous reviewers and technical editors of the Journal of Trees for their comments and corrections of the article.

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Author notes

  1. Ziyang Zhang, Ling Cheng and Weihua Zhang have contributed equally to this work.

Authors and Affiliations

  1. College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China

    Ziyang Zhang, Jianlin Hu, Yan Liu & Yuanzhen Lin

  2. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou, 510642, China

    Ziyang Zhang, Jianlin Hu, Yan Liu & Yuanzhen Lin

  3. Guangzhou Genedenovo Biotechnology Company Limited, Guangzhou, 510642, China

    Ling Cheng

  4. Guangdong Academy of Forestry, Guangzhou, 510640, China

    Weihua Zhang

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  1. Ziyang Zhang
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  2. Ling Cheng
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  3. Weihua Zhang
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  4. Jianlin Hu
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  5. Yan Liu
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Correspondence to Yuanzhen Lin.

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Zhang, Z., Cheng, L., Zhang, W. et al. Characterization of transcription activation domain of EcaICE1 and its interaction with EcaSIZ1 in Eucalyptus camaldulensis. Trees 34, 1243–1253 (2020). https://doi.org/10.1007/s00468-020-01994-9

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  • DOI: https://doi.org/10.1007/s00468-020-01994-9

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