Fabrication and evaluation of polylactic acid/pectin composite scaffold via freeze extraction for tissue engineering

Mohd Syahir Anwar Hamzah; Saiful Izwan Abd Razak; Mohammed Rafiq Abdul Kadir; Siti Pauliena Mohd Bohari; Nadirul Hasraf Mat Nayan; Joseph Sahaya Thangaraj Anand

doi:10.1515/polyeng-2019-0377

Published by De Gruyter April 30, 2020

Fabrication and evaluation of polylactic acid/pectin composite scaffold via freeze extraction for tissue engineering

Mohd Syahir Anwar Hamzah , Saiful Izwan Abd Razak , Mohammed Rafiq Abdul Kadir , Siti Pauliena Mohd Bohari , Nadirul Hasraf Mat Nayan and Joseph Sahaya Thangaraj Anand

From the journal Journal of Polymer Engineering

https://doi.org/10.1515/polyeng-2019-0377

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Abstract

This work reports the fabrication and characterizations of porous scaffold made up of polylactic acid (PLA) with the inclusion of pectin (1, 3, 5, 7, 9, 11 wt%) for potential tissue engineering material. The composite scaffold was prepared using a facile method of freeze extraction. Based on the physical evaluations, the scaffold was suggested to be optimum at 5 wt% of pectin loading. Water contact angle of the scaffold was significantly reduced to 46.5^o with the inclusion of 5 wt% of pectin. Morphological and topographic of the PLA scaffold revealed that the pectin induced more porous structure and its surface became rougher which was suitable for cell attachment and proliferation. In vitro studies of the PLA/pectin composite scaffold using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidelt (MTT) assay revealed good biocompatibility whereas Live-Dead kit assay resulted in 91% cell viability after 7 days of incubation.

Keywords: composite scaffold; freeze extraction; pectin; polylactic acid; tissue engineering

Corresponding author: Saiful Izwan Abd Razak, Centre for Advanced Composite Materials, Universiti Teknologi Malaysia, 81300, Skudai, Johor, Malaysia; and BioInspired Device and Tissue Engineering Research Group, School of Biomedical Engineering and Health Sciences, Faculty of Engineering, 81300, Skudai, Johor, Malaysia, E-mail: saifulizwan@utm.my

Funding source: Universiti Teknologi Malaysia

Award Identifier / Grant number: 03G83, 04G53

Research funding: Financial support from the Universiti Teknologi Malaysia grant number 04G53 and 03G83 are gratefully acknowledged.

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Received: 2019-12-12

Accepted: 2020-03-15

Published Online: 2020-04-30

Published in Print: 2020-05-26

Fabrication and evaluation of polylactic acid/pectin composite scaffold via freeze extraction for tissue engineering

Abstract

References

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