Abstract
Pomegranate peel, a by-product of pomegranate fruit is rich in antioxidants. It is being used as an animal feed or discarded to the environment, which could cause an environmental problem. A new integrated process of maceration and freeze concentration was studied to recover the antioxidants from the pomegranate peel. The low temperature used in the freeze concentration assists in concentrating a high quality of antioxidants. The concentrated peel solution was tested for an antioxidant activity via 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. It was found that methanol gave the best extraction of antioxidants from pomegranate peel. The highest antioxidant recovery with DPPH radical scavenging of 96.14 % was obtained at −16 °C of coolant temperature and 30 min of freezing time (p < 0.05). The integrated process has proven to be an effective method to recover the antioxidants from pomegranate peel and can be applied to recover other bioactive compounds.
Abbreviations and Nomenclature
- DPPH
2,2-diphenyl-1-picrylhydrazyl
- ANOVA
Analysis of variance
- P
Significant differences among mean values
- A0
Absorbance of control
- A1
Absorbance of sample
Acknowledgements
The authors are thankful to Universiti Teknologi PETRONAS for the financial support through YUTP-FRG with Cost Centre 015LC0-079 and HICoE-Centre for Biofuel and Biochemical Research for facilities support.
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