Abstract
No genomic sequence of Mycobacterium isolated from orchids has been reported yet; therefore, this study intends to analyze the complete genomic sequence of a growth-promoting Mycobacterium from orchid Doritaenopsis. Mycobacterium strain Mya-zh01 was isolated from the flower stalk of Doritaenopsis Jiuhbao Red Rose. Our results show that Mya-zh01 can effectively produce and secrete the plant growth hormone indole-3-acetic acid (IAA). Inoculation of Mya-zh01 increased root number and length, plant height, leaf number, and leaf length in Doritaenopsis. Furthermore, inoculation of Mya-zh01 promotes seed germination in Doritaenopsis. We sequenced and assembled chromosome for Mya-zh01 (5,027,704 bp with 68.48% GC content), which was predicted to encode 4968 proteins with functions in oxidation reduction, growth, plasma membrane, ATP and DNA binding, carbon metabolism and biosynthesis of amino acids pathways. Mya-zh01 may trap iron from nature or host cells to facilitate the growth of the orchids by producing two siderophores (Mycobactin and Nocobactin NA). Four pathways (tryptamine, indole-3-acetamide, indole-3-pyruvate, and flavin monooxygenase) and seven enzymes [tryptophan synthase alpha chain, tryptophan synthase beta chain, amidase, monoamine oxidase, indole-3-pyruvate monooxygenase, indole-3-pyruvate decarboxylase and aldehyde dehydrogenase (NAD +)] involved in IAA biosynthesis were predicted in Mya-zh01 genome. In conclusion, this study demonstrated the significance of Mya-zh01 in facilitating plant growth and seed germination in Doritaenopsis by IAA biosynthesis, which provides a new insight into the mechanism of plant–bacteria interaction in Doritaenopsis.
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Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- IAA:
-
Indole-3-acetic acid
- Trp:
-
Tryptophan
- IAM:
-
Indole-3-acetamide
- IAN:
-
Indole-3-acetonitrile
- IPyA:
-
Indole-3-pyruvate
- YUCCA:
-
Indole-3-pyruvate monooxygenase
- IPDC:
-
Indole-3-pyruvate decarboxylase
- TAM:
-
Tryptamine
- YUC:
-
Flavin monooxygenase
- TSO:
-
Tryptophan side-chain oxidase
- OAB:
-
Orchid-associated bacteria
- PGPR:
-
Plant growth-promoting rhizobacteria
- PAHs:
-
Polycyclic aromatic hydrocarbons
- trpA:
-
Tryptophan synthase alpha chain
- trpB:
-
Tryptophan synthase beta chain
- amiE:
-
Amidase
- MAO:
-
Monoamine oxidase
- NAD + ALDH:
-
Aldehyde dehydrogenase
- HPLC:
-
High-performance liquid chromatography
- Dtps.:
-
Doritaenopsis
- C. vestita :
-
Calanthe vestita
- D. moschatum :
-
Dendrobium moschatum
- A. papilosa :
-
Acampe papilosa
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Pan, L., Chen, J., Ren, S. et al. Complete genome sequence of Mycobacterium Mya-zh01, an endophytic bacterium, promotes plant growth and seed germination isolated from flower stalk of Doritaenopsis. Arch Microbiol 202, 1965–1976 (2020). https://doi.org/10.1007/s00203-020-01924-w
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DOI: https://doi.org/10.1007/s00203-020-01924-w