Abstract
The complete genome sequences of two grapevine virus L (GVL) isolates collected from the wine grape cultivar Blanc du Bois (Vitis spp.: ‘Florida D 6-148’בCardinal’) in Texas were determined. The two genome sequences (excluding the polyA tail) were each 7594 nucleotide long and 99.7% identical to each other, but they shared only ~74% identity with those of previously published GVL isolates. Further analysis showed that the two Texas GVL isolates also diverged significantly from previously published isolates of the virus in each of the five ORFs at both the nucleotide and amino acid level, indicating that they represent a new phylogroup of this virus.
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Acknowledgements
This work was supported by funding from the United States Department of Agriculture-Agricultural Marketing Service, Specialty Crop Block Grant (SC-1920-24) and the Texas Wine Grape Growers Association, through Senate Bill 881 (SB881). We thank Mr. Jerry Watson for his cooperation and access to his Blanc du Bois vineyard in Austin County. We are also grateful to Mr. Jonathan Motloch, Ms. Regina Hernanadez, and Ms. Cecilia Villegas (Texas A&M University System) for help with sample processing, and Dr. Minsook Hwang and Dr. Kristian Stevens (UC Davis Foundation Plant Services) for help with HTS library preparation and bioinformatics analysis.
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Alabi, O.J., Appel, D.N., McBride, S. et al. Complete genome sequence analysis of a genetic variant of grapevine virus L from the grapevine cultivar Blanc du Bois. Arch Virol 165, 1905–1909 (2020). https://doi.org/10.1007/s00705-020-04682-8
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DOI: https://doi.org/10.1007/s00705-020-04682-8