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Glaciimonas soli sp. nov., a soil bacterium isolated from the forest of a high elevation mountain

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A Correction to this article was published on 04 January 2021

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Abstract

A Gram-negative, psychrophilic bacterium, designated strain GS1T, was isolated from a forest soil sample collected from the West Peak of Mt. Yushan, Yushan National Park, Taiwan. Cells grown in broth cultures were mostly non-motile and non-flagellated, whereas motile cells with monotrichous, subpolar flagella were also observed. The novel strain grew over a temperature range of 4–25 °C with optimum growth at 10–15 °C. It grew aerobically and was not capable of anaerobic growth by fermentation of d-glucose or other carbohydrates. Ubiquinone 8 was the predominant isoprenoid quinone. The major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol and dimethylaminoethanol. Cellular fatty acids were dominated by C16:1 ω7c (35.2%), C16:0 (19.5%), C18:1 ω7c (18.8%) and C17:0 ω7c cyclo (15.5%). The DNA G + C content was 49.2 mol% evaluated according to the genomic sequencing data. Strain GS1T shared more than 96.5% 16S rRNA gene sequence similarities with type strains of four Collimonas species (97.2–97.5%), three Glaciimonas species (97.3% for each of the three) and Oxalicibacterium solurbis (96.5%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GS1T formed a stable genus-level clade with type strains of species in the genus Glaciimonas in the family Oxalobacteraceae and GS1T was an outgroup with respect to these Glaciimonas species. Characteristically, strain GS1T could be easily distinguished from the recognised Glaciimonas species by exhibition of swimming motility with monotrichous, subpolar flagellum in some of the cells, ability to grow in NaCl at 2% but not at 3% and the distinguishable fatty acid profiles. On the basis of the polyphasic taxonomic data from this study, strain GS1T is considered to represent a novel species of the genus Glaciimonas, for which the name Glaciimonas soli sp. nov. is proposed. The type strain is GS1T (= JCM 33275T = BCRC 81091T).

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Acknowledgements

This study was supported by the Ministry of Science and Technology, Taiwan (Grant 105-2311-B-002-017). We thank the administration office of Yushan National Park for allowing our collection of soil samples in the Park.

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Author notes

  1. Wei-Sheng Huang, Li-Ting Wang, Jun-Ning Sun and Jwo-Sheng Chen have contributed equally in this study and should be considered as co-first authors.

Authors and Affiliations

  1. Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, 10617, Taiwan

    Wei-Sheng Huang, Jun-Ning Sun, Ssu-Po Huang & Wung Yang Shieh

  2. Bioresource Collection and Research Center, Food Industry Research and Development Institute, 331 Shih-Pin Rd., Hsinchu, 30062, Taiwan

    Li-Ting Wang, Shih-Ting Lin & Lina Huang

  3. College of Health Care, China Medical University, No. 91, Shyue-Shyh Rd, Taichung, Taiwan

    Jwo-Sheng Chen

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  1. Wei-Sheng Huang
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Contributions

W-SH and L-TW performed the phylogenetic, chemotaxonomic and genomic analyses, and prepared the figures and tables. J-NS performed isolation, and initial physiological and morphological analyses of the strain. S-PH, S-TL and LH authenticated the results from physiological and chemotaxonomic analyses. WYS collaborated with J-SC for supervision of the study and preparation of the manuscript.

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Correspondence to Wung Yang Shieh.

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Huang, WS., Wang, LT., Sun, JN. et al. Glaciimonas soli sp. nov., a soil bacterium isolated from the forest of a high elevation mountain. Antonie van Leeuwenhoek 113, 1213–1223 (2020). https://doi.org/10.1007/s10482-020-01428-z

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