Abstract
Objectives
To investigate the role of YAP in cyclic mechanical stress induced up-regulation of HIF-1α in rat cartilage chondrocytes.
Results
Our in vitro and in vivo experiments demonstrated that cyclic mechanical stress promoted HIF-1α and YAP proteins expression in a magnitude dependent manner. Cyclic mechanical stress at 4000μ strain exhibited most significant effect in promoting HIF-1α and YAP up-regulation. Activation of YAP using LPA significantly promoted HIF-1α stabilization and expression, while YAP siRNA treatment suppressed the up-regulation of HIF-1α induced by cyclic mechanical stress.
Conclusion
Our results indicated that cyclic mechanical stress promoted HIF-1α stabilization and YAP is involved in mechanical stress induced HIF-1α up-regulation.
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Data availability
All data generated or analysed during this study are included in this published article and its supplementary information files.
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Supporting information
Supplementary Table 1—Primer sequences used in the quantitative Real-time PCR.
Supplementary Table 2—The target sequences of YAP siRNAs.
Funding
This work was supported by grants from the National Natural Science Foundation of China (Grant Nos. 81702155 and 81874020).
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Jing, X., Yang, X., Zhang, W. et al. Mechanical loading induces HIF-1α expression in chondrocytes via YAP. Biotechnol Lett 42, 1645–1654 (2020). https://doi.org/10.1007/s10529-020-02910-4
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DOI: https://doi.org/10.1007/s10529-020-02910-4