X-ray structure of C-phycocyanin from Galdieria phlegrea: Determinants of thermostability and comparison with a C-phycocyanin in the entire phycobilisome

https://doi.org/10.1016/j.bbabio.2020.148236Get rights and content
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Highlights

  • The X-ray structure of C-phycocyanin from Galdieria phlegrea was solved.

  • Two structures of C-phycocyanin from Galdieria sulphuraria were re-refined.

  • GpPC structure was compared with that of a C-PC in the Porphyridium purpureum phycobilisome.

  • Structural determinants of GpPC thermostability were highlighted.

  • GpPC has an increased number of inter-(αβ)3/(αβ)3 salt bridges when compared to other C-PCs.

Abstract

Galdieria phlegrea is a polyextremophilic red alga belonging to Cyanidiophyceae. Galdieria phlegrea C-phycocyanin (GpPC), an abundant light-harvesting pigment with an important role in energy capture and transfer to photosystems, is the C-phycocyanin (C-PC) with the highest thermal stability described so far. GpPC also presents interesting antioxidant and anticancer activities. The X-ray structure of the protein was here solved. GpPC is a [(αβ)3]2 hexamer, with the phycocyanobilin chromophore attached to Cys84α, Cys82β and Cys153β. Details of geometry and interaction with solvent of the chromophores are reported. Comparison with the structure of a C-PC in the entire Porphyridium purpureum phycobilisome system reveals that linker polypeptides have a significant effect on the local structure of the chromophores environment. Comparative analyses with the structures of other purified C-PCs, which were carried out including re-refined models of G. sulphuraria C-PC, reveal that GpPC presents a significantly higher number of inter-trimer salt bridges. Notably, the higher number of salt bridges at the (αβ)3/(αβ)3 interface is not due to an increased number of charged residues in this region, but to subtle conformational variations of their side chains, which are the result of mutations of close polar and non-polar residues.

Keywords

C-phycocyanin
Protein thermostability
Crystal structure

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Present address: Department of Chemistry “Ugo Schiff”, University of Florence, Sesto Fiorentino, Italy.