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QTL and eQTL mapping associated with host response to Candidatus Liberibacter asiaticus in citrandarins

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Abstract

Huanglongbing (HLB) is a severe disease of citrus caused by the bacterium Candidatus Liberibacter. In America, the most common species is Candidatus Liberibacter asiaticus (CLas). In a previous study of the Citrus-HLB pathosystem, our group found differences in CLas multiplication in a population of hybrids obtained by hybridization between Citrus sunki Hort. Ex Tan. and Poncirus trifoliata (L.) Raf. cv. Rubidoux. The bacterial concentration was higher in C. sunki than in P. trifoliata. Thus, this study aims to map phenotypic (QTL) and gene expression (eQTL) data associated with host response to CLas in the linkage groups (LGs) of the previously constructed parental maps of C. sunki and P. trifoliata cv. Rubidoux. For the phenotypic analysis, we used a population of 79 F1 hybrids (termed citrandarins) between C. sunki and P. trifoliata. CLas and starch were quantified in the leaves of the plants two years after pathogen inoculation, allowing the classification of hybrids as resistant, tolerant, and susceptible. The expression of 14 candidate genes was measured in 72 hybrids of the population and used as expression data for the eQTL mapping. We located nine QTL and 52 eQTL in the C. sunki map and 17 QTL and 40 eQTL in the P. trifoliata map. The overlap of eQTL of the majority of genes with QTL from the phenotypic data indicates that the genes are related to the phenotype and are probably related to pathogen infection.

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Acknowledgements

This work was performed with the financial support of the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Processes nº 2011/18605-0 and 2018/00133-4) and the Instituto Nacional de Ciência e Tecnologia (INCT) de Genômica para Melhoramento de Citros (Processes: CNPq 465440/2014-2 and Fapesp 2014/50880-0). The authors Tatiany Aparecida Teixeira Soratto and Maiara Curtolo are recipient of research fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes) and FAPESP 2016/22133-0, respectively.

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Authors and Affiliations

  1. Centro APTA Citros Sylvio Moreira, Instituto Agronômico de Campinas, 13490-970, Cordeirópolis, SP, Brazil

    Tatiany Aparecida Teixeira Soratto, Maiara Curtolo, Ana Lúcia Dezotti, Rômulo Pedro Macêdo Lima, Marcos Antonio Machado & Mariângela Cristofani-Yaly

  2. Laboratório de Bioinformática, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88040-970, Florianópolis, SC, Brazil

    Tatiany Aparecida Teixeira Soratto

  3. Universidade Estadual de Campinas, 13083-862, Campinas, SP, Brazil

    Maiara Curtolo

  4. Hill Laboratories, 3204, Hamilton, New Zealand

    Samanta Marengo

  5. Departamento de Genética, Instituto de Biociências, Universidade do Estado de São Paulo, 18618-000, Botucatu, SP, Brazil

    Rômulo Pedro Macêdo Lima

  6. Centro de Ciências Agrárias, Universidade Federal de São Carlos, 13600-970, Araras, SP, Brazil

    Rodrigo Gazaffi

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  1. Tatiany Aparecida Teixeira Soratto
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  7. Marcos Antonio Machado
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  8. Mariângela Cristofani-Yaly
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Contributions

MCY and MAM planned and supervised the study. TAST and MCY contributed to the design and execution of the project. TAST, MC, RG and SM performed the phenotype evaluation and the QTL and eQTL mapping analysis. TAST, MC and ALD participated in extraction of plant RNA, cDNA synthesis and validation of genes by RT-qPCR. RPML and TAST performed gene expression analysis. RPML, MCY, MC and TAST drafted and critically revised the manuscript and provided intellectual input. All authors read and approved the final manuscript.

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Correspondence to Mariângela Cristofani-Yaly.

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Soratto, T.A.T., Curtolo, M., Marengo, S. et al. QTL and eQTL mapping associated with host response to Candidatus Liberibacter asiaticus in citrandarins. Trop. plant pathol. 45, 626–645 (2020). https://doi.org/10.1007/s40858-020-00372-7

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