Abstract
Glycerol plasticized soy protein isolate (SPI) films at different contents (1 to 5% w/w w.r.t SPI) of nalidixic acid (NA) were fabricated by solution casting method. SPI films and NA incorporated SPI films were structurally and mechanically characterized by Fourier transform infrared spectroscopy (FTIR) and mechanical properties, respectively. Thermomechanical and water uptake studies were also carried out for NA incorporated SPI films. The results from FTIR study indicated the presence of NA in SPI as evidenced by generation of two additional peaks at 1259 cm− 1 and 800 cm− 1 attributed to C–C stretching vibration and the deformation of the heterocyclic aromatic ring, respectively. At 4% NA, the tensile stress and tensile modulus of SPI films increased from 5.18 MPa to 8.57 MPa and 57.14 MPa to 93.90 MPa, respectively. Antibacterial activity of NA incorporated SPI films against E. coli and L. monocytogenes were studied. The antibacterial studies of NA incorporated SPI film showed the clear zone of inhibition against both the bacteria. Docking studies were performed for SPI in order to evaluate their binding affinity with NA and similar type of aromatic acids such as mandelic and benzilic acid with which SPI films have been prepared earlier by our group. After docking, the ligands in form of aromatic acids were evaluated according to their binding energy and the interaction pattern between SPI and aromatic acids. The docking results indicated that nitrogen containing heterocyclic ring moiety remained intact during interaction of SPI and NA. This work will be helpful in fabricating NA incorporated SPI film from the renewable resources with significant antibacterial properties and reasonable mechanical properties.
Similar content being viewed by others
References
Jagadeesh D, Reddy DJP, Rajulu AV (2011) J Polym Environ 19:248–253
Ma ZS, Liu DG, Zhu Y, Li ZH, Li ZX, Tian HF, Liu HQ (2016) Carbohydr Polym 144:230–237
Muthulakshmi L, Rajini N, Nellaiah H, Kathiresan T, Jawaid M, Rajulu AV (2017) Int J Biol Macromol 95:1064–1071
Tian H, Guo G, Fu X, Yao Y, Yuan L, Xiang A (2018) Int J Biol Macromol 120:475–490
Rani S, Kumar R (2019) J Polym Environ 27:1613–1628
Zhao S, Yao JR, Fei X, Shao ZZ, Chen X (2013) Mater Lett 95:142–144
Wang L, Li J, Zhang S, Shi J (2016) Materials 9:193
Saenghirunwattana P, Noomhorm A, Rungsardthong V (2014) Ind Crop Prod 60:144–150
Liu F, Zou HL, Hu JW, Liu HB, Peng JB, Chen YW, Lu FH, Huo YP (2016) Chem Eng J 287:410–418
Souzandeh H, Johnson KS, Wang Y, Bhamidipaty K, Zhong WH (2016) Appl Mater Interfaces 8:20023–20031
Guo G, Tian H, Wu Q (2019) Polym Compos 40:3768–3776
Li Y, Chen H, Dong YM, Li K, Li L, Li JZ (2016) Ind Crop Prod 82:133–140
Han YY, Wang LJ (2016) RSC Adv 6:112317–112324
Padgett T, Han IY, Dawson PL (1998) J Food Prot 61:1330–1335
Hoseinnejad M, Jafari SM, Katouzian I (2017) Crit Rev Microbiol 44:1–21
Zhao Y, He M, Zhao L, Wang S, Li Y, Gan L, Li M, Xu L, Chang PR, Anderson DP, Chen Y (2016) Appl Mater Interfaces 8:2781–2795
Eswaranandam S, Hettiarachchy NS, Johnson MG (2004) J Food Sci 69:79–84
Alves MM, Gonçalves MP, Rocha CM (2017) LWT Food Sci Technol 80:409–415
Kumar R, Zhang L (2008) Biomacromol 9:2430–2437
Kumar R, Zhang L (2009) Ind Crop Prod 29:485–494
Kumar R, Anandjiwala RD, Kumar A (2016) J Therm AnalCalorim 123:1273–1279
Bai H, Kumar R, Yang C, Liu X, Zhang L (2010) Polym Compos 18:197–203
Wang Z, Kanga H, Zhang W, Zhang S, Li J (2017) Appl Surf Sci 401:271–282
Anna F, Sergi M, Ernest G, Jordi V (2009) Microb Biotechnol 2:40–61
Katie J, Aldred R, Kerns J, Neil O (2014) Biochemistry 53:1565–1574
Vashist J, Vishvanath K, Renuka K, Arti Y, Ragothaman SN, Rajeswari MR (2009) Indian J Biochem Bio 46:147–153
Shaji D (2018) J Pharma Ana 8:318–323
Odani S, Koide T, Ono T (1987) J Bio Chem 262:10502–10505
Trott O, Olson AJ (2010) J Comput Chem 31:455–461
Kumar R, Wang L, Zhang L (2009) J Appl Polym Sci 111:970–977
Acknowledgements
Authors are grateful to the DST-FIST (File No. SR/FST/LS-I/2019/513) funded Department of Biotechnology, CUSB, Gaya for providing computational facility.
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Rani, S., Singh, A.K., Paswan, R.R. et al. Preparation, Characterization and Antibacterial Evaluation of Soy Protein Isolate Biopolymeric Films Loaded with Nalidixic Acid. J Polym Environ 28, 1841–1850 (2020). https://doi.org/10.1007/s10924-020-01729-4
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10924-020-01729-4