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The effects of tricaine mesylate on arthropods: crayfish, crab and Drosophila

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Invertebrate Neuroscience

Abstract

Tricaine mesylate, also known as MS-222, was investigated to characterize its effects on sensory neurons, synaptic transmission at the neuromuscular junction, and heart rate in invertebrates. Three species were examined: Drosophila melanogaster, blue crab (Callinectes sapidus), and red swamp crayfish (Procambarus clarkii). Intracellular measures of action potentials in motor neurons of the crayfish demonstrated that MS-222 dampened the amplitude, suggesting that voltage-gated Na + channels are blocked by MS-222. This is likely the mechanism behind the reduced activity measured in sensory neurons and depressed synaptic transmission in all three species as well as reduced cardiac function in the larval Drosophila. To address public access to data, a group effort was used for analysis of given data sets, blind to the experimental design, to gauge analytical accuracy. The determination of a threshold in analysis for measuring extracellular recorded sensory events is critical and is not easily performed with commercial software.

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References

  • Alexandrowicz JS (1967) Receptor organs in the coxal region of Palinurus vulgaris. J Marine Biol Assoc UK 47:415–432

    Google Scholar 

  • Applegate JR Jr, Dombrowski DS, Christian LS, Bayer MP, Harms CA, Lewbart GA (2016) Tricaine methanesulfonate (ms-222) sedation and anesthesia in the purple-spined sea urchin (Arbacia punctulata). J Zoo Wildlife Med 47(4):1025–1033. https://doi.org/10.1638/2015-0288.1

    Article  Google Scholar 

  • Archibald KE, Scott GN, Bailey KM, Harms CA (2019) 2-phenoxyethanol (2-pe) and tricaine methanesulfonate (ms-222) immersion anesthesia of American horseshoe crabs (Limulus polyphemus). J Zoo Wildl Med 50(1):96–106. https://doi.org/10.1638/2018-0085

    Article  PubMed  Google Scholar 

  • Attili S, Hughes SM (2014) Anaesthetic tricaine acts preferentially on neural voltage-gated sodium channels and fails to block directly evoked muscle contraction. PLoS ONE 9(8):e103751. https://doi.org/10.1371/journal.pone.0103751

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Atwood HL, Cooper RL (1996) Assessing ultrastructure of crustacean and insect neuromuscular junctions. J Neurosci Methods 69:51–58

    CAS  PubMed  Google Scholar 

  • Bagnéris C, DeCaen PG, Naylor CE, Pryde DC, Nobeli I, Clapham DE, Wallace BA (2014) Prokaryotic NavMs channel as a structural and functional model for eukaryotic sodium channel antagonism. Proc Natl Acad Sci USA 111:8428–8433

    PubMed  Google Scholar 

  • Baierlein B, Thurow AL, Atwood HL, Cooper RL (2011) Membrane potentials, synaptic responses, neuronal circuitry, neuromodulation and muscle histology using the crayfish: student laboratory exercises. J Vis Exp 47, http://www.jove.com/Details.php?ID=2322. https://doi.org/10.3791/2325

  • Bakshi A, Patrick LE, Wischusen EW (2016) A framework for implementing course-based undergraduate research experiences (CUREs) in freshman biology labs. Am Biol Teach 78(6):448–455

    Google Scholar 

  • Barron AB (2000) Anaesthetising for behavioural studies. J Insect Physiol 46(4):439–442

    CAS  PubMed  Google Scholar 

  • Bewick GS, Banks RW (2015) Mechanotransduction in the muscle spindle. Pflügers Archiv 467(1):175–190. https://doi.org/10.1007/s00424-014-1536-9

    Article  CAS  PubMed  Google Scholar 

  • Bierbower SM, Cooper RL (2009) Measures of heart and ventilatory rates in freely moving crayfish. J Vis Exp 32, http://www.jove.com/index/details.stp?id=1594. https://doi.org/10.3791/1594

  • Bowker JD, Trushenski JT, Glover DC, Carty DG, Wandelear N (2014) Sedative options for fish research: a brief review with new data on sedation of warm-, cool-, and coldwater fishes and recommendations for the drug approval process. Rev Fish Biol Fish 25(1):147–163. https://doi.org/10.1007/s11160-014-9374-6

    Article  Google Scholar 

  • Brodbelt DC (2009) Perioperative mortality in small animal anaesthesia. Veterinary J (Lond) 182:152–161

    Google Scholar 

  • Brown PB, White MR, Chaille J, Russell M, Oseto C (1996) Evaluation of three anesthetic agents for crayfish (Orconectes virilis). J Shellfish Res 15(2):433–435

    Google Scholar 

  • Butterworth JFT, Strichartz GR (1990) Molecular mechanisms of local anesthesia: a review. Anesthesiology 72:711–734

    CAS  PubMed  Google Scholar 

  • Cakir Y, Strauch SM (2005) Tricaine (MS-222) is a safe anesthetic compound compared to benzocaine and pentobarbital to induce anesthesia in leopard frogs (Rana pipiens). Pharmacol Rep 57:467–474

    PubMed  Google Scholar 

  • Catterall WA, Cestele S, Yarov-Yarovoy V, Yu FH, Konoki K, Scheuer T (2007) Voltage-gated ion channels and gating modifier toxins. Toxicon 49:124–141

    CAS  PubMed  Google Scholar 

  • Clare JDJ, Townsend PA, Anhalt-Depies C, Locke C, Stenglein JL, Frett S, Martin KJ, Singh A, Van Deelen TR, Zuckerberg B (2019) Making inference with messy (citizen science) data: when are data accurate enough and how can they be improved? Ecol Appl 29(2):e01849. https://doi.org/10.1002/eap.1849

    Article  PubMed  Google Scholar 

  • Collymore C, Banks EK, Turner PV (2016) Lidocaine hydrochloride compared with ms222 for the euthanasia of zebrafish (Danio rerio). J Am Assoc Lab Anim Sci 55(6):816–820

    PubMed  PubMed Central  Google Scholar 

  • Cooper RL (2008) Mapping proprioceptive neurons on chordotonal organs in the crab. Cancer magister Crustaceana 81(4):447–475

    Google Scholar 

  • Cooper AS, Cooper RL (2009). Historical view and demonstration of physiology at the NMJ at the crayfish opener muscle. J Vis Exp 33. http://www.jove.com/index/details.stp?id=1595. https://doi.org/10.3791/1595

  • Cooper RL, Govind CK (1991) Axon composition of the proprioceptive PD nerve during growth and regeneration of lobster claws. J Exp Zool 260:181–193

    Google Scholar 

  • Cooper RL, Hartman HB (1999) Quantification of responses from proprioceptive neurons in the limbs of the crab, Cancer magister. J Exp Zool 284:629–636

    CAS  PubMed  Google Scholar 

  • Cooper AS, Rymond KE, Ward MA, Bocook EL, Cooper RL (2009) Monitoring heart function in larval Drosophila melanogaster for physiological studies. J Vis Exp 32, http://www.jove.com/index/details.stp?id=1596

  • Cooper AS, Leksrisawat B, Mercier AJ, Gilberts AB, Cooper RL (2011) Physiological experimentations with the crayfish hindgut. J Vis Exp 47, http://www.jove.com/details.php?id=2324doi: https://doi.org/10.3791/2324

  • Coyle SD, Dasgupta S, Tidwell JH, Beavers T, Bright LA, Yasharian DK (2005) Comparative Efficacy of Anesthetics for the Freshwater Prawn Macrobrachiurn rosenbergii. J World Aquac Soc 36:282–290. https://doi.org/10.1111/j.1749-7345.2005.tb00332.x

    Article  Google Scholar 

  • Crider ME, Cooper RL (2000) Differential facilitation of high- and low-output nerve terminals from a single motor neuron. J Appl Physiol 88:987–996

    CAS  PubMed  Google Scholar 

  • Davies TG, Field LM, Usherwood PN, Williamson MS (2007) A comparative study of voltage-gated sodium channels in the Insecta: implications for pyrethroid resistance in Anopheline and other Neopteran species. Insect Mol Biol 16:361–375

    CAS  PubMed  Google Scholar 

  • Davis DJ, Klug J, Hankins M, Doerr HM, Monticelli SR, Song A, Gillespie CH, Bryda EC (2015) Effects of clove oil as a euthanasia agent on blood collection efficiency and serum cortisol levels in Danio rerio. J Am Assoc Lab Anim Sci 54(5):564–567

    PubMed  PubMed Central  Google Scholar 

  • DayaramV, Malloy C, Martha S, Alvarez B, Chukwudolue I, Dabbain N, Mahmood DD, Goleva S, Hickey T, Ho A, King M, Kington P, Mattingly M, Potter S, Simpson L, Spence A, Uradu H, Van Doorn JL, Cooper RL (2017) Stretch activated channels in propriocep-tive chordotonal organs of crab and crayfish are sensitive to Gd3 + but not amiloride, ruthenium red or low pH. IMPLUSE The Premier Undergraduate Neuroscience Journal, https://impulse.appstate.edu/issues/2017

  • de Castro C, Titlow J, Majeed ZR, Cooper RL (2014) Analysis of various physiological salines for heart rate, CNS function, and synaptic transmission at neuromuscular junctions in Drosophila melanogaster larvae. J Comp Physiol A 200:83–92

    Google Scholar 

  • de Castro C, Titlow JS, Majeed ZR, Malloy C, King KE, Cooper RL (2019) Chemical and mechanical factors required for maintaining cardiac rhythm in Drosophila melanogaster larva. J Entomol 16(2):62–73

    Google Scholar 

  • Desai-Shah M, Viele K, Sparks G, Nadolski J, Hayden B, Srinivasan VK, Cooper RL (2008) Assessment of synaptic function during short-term facilitation in motor nerve terminals in the crayfish. Open Neurosci J 2:24–35

    CAS  Google Scholar 

  • Desai-Shah M, Papoy AR, Ward M, Cooper RL (2010) Roles of the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase, plasma membrane Ca2+-ATPase and Na+/Ca2+ exchanger in regulation of heart rate in larval Drosophila. Open Physiol J 3:16–36

    CAS  Google Scholar 

  • Diggles K (2018) Review of some scientific issues related to crustacean welfare. International Council for the Exploration of the Sea Journal of Marine Science. fsy058, https://doi.org/10.1093/icesjms/fsy058

  • EFSA (2005). Aspects of the biology and welfare of animals used for experimental and other scientific purposes. EFSA J 292: 1–46. https://ec.europa.eu/environment/chemicals/lab_animals/pdf/efsa_opinion.pdf

  • Flecknell PA (1984) The relief of pain in laboratory animals. Lab Anim 18(2):147–160. https://doi.org/10.1258/002367784780891226

    Article  CAS  PubMed  Google Scholar 

  • Frazier DT, Narahashi T (1975) Tricaine (MS-222): effects on ionic conductances of squid axon membranes. Eur J Pharmacol 33(2):313–317

    CAS  PubMed  Google Scholar 

  • Gaynor JS, Dunlop CI, Wagner AE, Wertz EM, Golden AE, Demme WC (1999) Complications and mortality associated with anesthesia in dogs and cats. J Am Anim Hosp Assoc 35(1):13–17. https://doi.org/10.5326/15473317-35-1-13

    Article  CAS  PubMed  Google Scholar 

  • Gilbert PW, Wood FG (1957) Method of anesthetizing large sharks and rays safely and rapidly. Science 126(3266):212–213. https://doi.org/10.1126/science.126.3266.212

    Article  CAS  PubMed  Google Scholar 

  • Gu GG, Singh S (1995) Pharmacological analysis of heartbeat in Drosophila. J Neurobiol 28:269–280

    CAS  PubMed  Google Scholar 

  • Guttman D (1967) MS-222 Sandoz as an anesthetic for black fly larvae (Diptera: Simuliidae). J Med Entomol 4(4):477–478

    CAS  PubMed  Google Scholar 

  • Hartman HB, Boettiger EG (1967) The functional organization of the propus-dactylus organ in Cancer irroratus Say. Comp Biochem Physiol 22:651–663

    Google Scholar 

  • Hartman HB, Cooper RL (1994) Regeneration and molting effects on a proprioceptor organ in the Dungeness crab, Cancer magister. J Neurobiol 25:461–471

    CAS  PubMed  Google Scholar 

  • Håstein T, Scarfe AD, Lund VL (2005) Science-based assessment of welfare: aquatic animals. Revue scientifique et technique (International Office of Epizootics) 24(2):529–547

    Google Scholar 

  • He P, Southard RC, Whiteheart SW, Cooper RL (1999) Role of alpha-SNAP in promoting efficient neurotransmission at the crayfish neuromuscular junction. J Neurophysiol 82:3406–3416

    CAS  PubMed  Google Scholar 

  • Holsinger RC, Cooper RL (2012) Effect of environment and modulators on hindgut and heart function in invertebrates: Crustaceans and Drosophila. Tested Studies for Laboratory Teaching, Volume 33 (K. McMahon, Editor). Proceedings of the 33rd Conference of the Association for Biology Laboratory Education (ABLE). http://www.ableweb.org/volumes/vol-33/v33reprint.php?ch=7

  • Javahery S, Nekoubin H, Moradlu AH (2012) Effect of anaesthesia with clove oil in fish. Fish Physiol Biochem 38:1545–1552

    CAS  PubMed  Google Scholar 

  • Johnson BR, Wyttenbach RA, Hoy RR (2014) Crustaceans as model systems for teaching neuroscience: past, present, and future. In: Derby C, Thiel M (eds) Nervous systems and control of behavior, the natural history of the Crustacea. Oxford University Press, New York

    Google Scholar 

  • Kuffler SW (1954) Mechanisms of activation and motor control of stretch receptors in lobster and crayfish. J Neurophysiol 17:558–574

    CAS  PubMed  Google Scholar 

  • Kurdyak P, Atwood HL, Stewart BA, Wu C-F (1994) Differential physiology and morphology of motor axons to ventral longitudinal muscle in larval Drosophila. J Comp Neurol 350:463–472

    CAS  PubMed  Google Scholar 

  • Lea T (1996) Caffeine and micromolar Ca2 + concentrations can release Ca2 + from ryanodine-sensitive stores in crab and lobster striated muscle fibres. J Exp Biol 199(11):2419–2428

    CAS  PubMed  Google Scholar 

  • Lea F, Malheiro B, González-Vélez H, Burguillo JC (2017) Trust-based modelling of multi-criteria crowdsourced data. Data Sci Eng 2(3):199–209

    Google Scholar 

  • Lee MH, Yeon KY, Park CK, Li HY, Fang Z, Kim MS, Choi SY, Lee SJ, Lee S, Park K, Lee JH, Kim JS, Oh SB (2005) Eugenol inhibits calcium currents in dental afferent neurons. J Dent Res 84:848–851

    CAS  PubMed  Google Scholar 

  • Lee S, Goodchild SJ, Ahern CA (2012) Local anesthetic inhibition of a bacterial sodium channel. J Gen Physiol 139:507–516

    CAS  PubMed  PubMed Central  Google Scholar 

  • Leksrisawat B, Cooper AS, Gilberts AB, Cooper RL (2010) Response properties of muscle receptor organs in the crayfish abdomen: A student laboratory exercise in proprioception. J Vis Exp 45 http://www.jove.com/index/details.stp?id=2323. https://doi.org/10.3791/2323

  • Li H, Harrison D, Jones G, Jones D, Cooper RL (2001) Alterations in development, behavior, and physiology in Drosophila larva that have reduced ecdysone production. J Neurophysiol 85:98–104

    CAS  PubMed  Google Scholar 

  • Lierz M, Korbel R (2012) Anesthesia and analgesia in birds. J Exotic Pet Med 21(1):44–58. https://doi.org/10.1053/j.jepm.2011.11.008

    Article  Google Scholar 

  • Linn MC, Palmer E, Baranger A, Gerard E, Stone E (2015) Undergraduate research experiences: impacts and opportunities. Science 347:1261757

    PubMed  Google Scholar 

  • Listerman L, Deskins J, Bradacs H, Cooper RL (2000) Measures of heart rate during social interactions in crayfish and effects of 5-HT. Compa Biochem Physiol A 125:251–264

    CAS  Google Scholar 

  • Majeed ZR, Nichols CD, Cooper RL (2013a) 5-HT stimulation of heart rate in Drosophila does not act through cAMP as revealed by pharmacogenetics. J Appl Physiol 115:1656–1665

    CAS  PubMed  PubMed Central  Google Scholar 

  • Majeed ZR, Titlow J, Hartman HB, Cooper RL (2013b) Proprioception and tension receptors in crab limbs: student laboratory exercises. J Vis Exp 80:e51050. https://doi.org/10.3791/51050

    Article  Google Scholar 

  • Majeed ZR, Stacy A, Cooper RL (2014) Pharmacological and genetic identification of serotonin receptor subtypes on Drosophila larval heart and aorta. J Comp Physiol B 184:205–219

    CAS  PubMed  Google Scholar 

  • Majeed Z, Koch F, Morgan J, Anderson H, Wilson J, Cooper RL (2017) A novel educa-tional module to teach neural circuits for college and high school students: NGSS-neurons, genetics, and selective stimulations. F1000Research: Immediate & Transparent Publishing for Life Scientists. F1000 Research Ltd, Middlesex House, 34-42 Cleveland St, London W1T 4LB, UK.https://f1000research.com/articles/6-117/v1

  • Malloy CA, Ritter K, Robinson J, English C, Cooper RL (2015) Pharmacological identification of cholinergic receptor subtypes on Drosophila melanogaster larval heart. J Comp Physiol B 186:45–57

    PubMed  Google Scholar 

  • Malloy C, DayaramV Martha S, Alvarez B, Chukwudolue I, Dabbain N, Mahmood DD, Goleva S, Hickey T, Ho A, King M, Kington P, Mattingly M, Potter S, Simpson L, Spence A, Uradu H, Van Doorn JL, Weineck K, Cooper RL (2017) The effects of potassium and muscle homogenate on proprioceptive responses in crayfish and crab. J Exp Zool 327(6):366–379

    CAS  Google Scholar 

  • Mendelson M (1963) Some factors in the activation of crab movement receptors. J Exp Biol 40:157–169

    Google Scholar 

  • Ramlochansingh C, Branoner F, Chagnaud BP, Straka H (2014) Efficacy of tricaine methanesulfonate (MS-222) as an anesthetic agent for blocking sensory-motor responses in Xenopus laevis tadpoles. PLoS ONE 9(7):e101606. https://doi.org/10.1371/journal.pone.0101606

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Robinson MM, Martin JM, Atwood HL, Cooper RL (2011). Modeling biological membranes with circuit boards and measuring conduction velocity in axons: Student laboratory exercises. J Vis Exp 47, http://www.jove.com/details.php?id=2325. https://doi.org/10.3791/2325

  • Roth B, Øines S (2010) Stunning and killing of edible crabs (Cancer pagurus). Anim Welf 19:287–294

    CAS  Google Scholar 

  • Rydqvist B, Lin JH, Sand P, Swerup C (2007) Mechanotransduction and the crayfish stretch receptor. Physiol Behav 92(1–2):21–28

    CAS  PubMed  Google Scholar 

  • Schmit O, Mezquita F (2010) Experimental test on the use of MS-222 for ostracod anaesthesia: concentration, immersion period and recovery time. J Limnol 69(2):350–352. https://doi.org/10.3274/JL10-69-2-N1

    Article  Google Scholar 

  • Sénatore S, Reddy VR, Sémériva M, Perrin L, Lalevée N (2010) Response to mechanical stress is mediated by the TRPA channel painless in the Drosophila heart. PLoS Genet 6(9):e1001088. https://doi.org/10.1371/journal.pgen.1001088

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • SOFIA 2018 http://www.fao.org/publications/sofia/en/ (Accessed on Aug. 20, 2019)

  • Sømme LS (2005) Sentience and pain in invertebrates. Report to Norwegian Scientific Committee for Food Safety. Norwegian University of Life Sciences, Oslo. Website: vkm.no/dav/0327284150.pdf

  • Stanback M, Stanback AE, Akhtar S, Basham R, Chithrala B, Collis B, Heberle BA, Higgins E, Lane A, Marella S, Ponder M, Raichur P, Silverstein A, Stanley C, Vela K, Cooper RL (2019). The effect of lipopolysaccharides on primary sensory neurons in crustacean models. IMPLUSE https://impulse.appstate.edu/articles/2019/effect-lipopolysaccharides-primary-sensory-neurons-crustacean-models

  • Stewart BA, Atwood HL, Renger JJ, Wang J, Wu CF (1994) Improved stability of Drosophila larval neuromuscular preparation in haemolymph-like physiological solutions. J Comp Physiol A 175:179–191

    CAS  PubMed  Google Scholar 

  • Stoskopf MK (2006) Coelenterates. In: Lewbart GA (ed) Invertebrate medicine, 1st edn. Blackwell Publishing, Ames, pp 19–52

    Google Scholar 

  • Thenappan A, Dupont-Versteegden EE, Cooper RL (2019) An educational model for understanding acute deep tissue injury of motor units. Journal of Young Investigators 36(5):62–71

    Google Scholar 

  • Titlow J, Majeed ZR, Hartman HB, Burns E, Cooper RL (2013a) Neural circuit recording from an intact cockroach nervous system. J Vis Exp 80:e51050. https://doi.org/10.3791/51050

    Article  Google Scholar 

  • Titlow JS, Rufer JM, King KE, Cooper RL (2013b) Pharmacological analysis of dopamine modulation in the Drosophila melanogaster larval heart. Physiol Rep 1(2):e00020. https://doi.org/10.1002/phy2.20

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wayson KA (1976) Studies on the comparative pharmacology and selective toxicity of tricaine methanesulfonate: metabolism as a basis of the selectivity toxicity in poikilotherms. J Pharmacol Exp Ther 198(3):695–708

    CAS  PubMed  Google Scholar 

  • Weineck K, Ray AJ, Fleckenstein L, Medley M, Dzubuk N, Piana E, Cooper RL (2018) Physiological changes as a measure of crustacean welfare under different standardized stunning techniques: cooling and electroshock. Animals 8(9):e158. https://doi.org/10.3390/ani8090158

    Article  PubMed  Google Scholar 

  • Weineck K, Stanback AE, Cooper RL (2019) The effects of eugenol as an anesthetic for an insect: Drosophila, adult, larval heart rate and synaptic transmission. Article 54. In: McMahon K editor. Tested studies for laboratory teaching. Volume 40. Proceedings of the 39th Conference of the Association for Biology Laboratory Education (ABLE). http://www.ableweb.org/volumes/vol-40/?art=# Vol 40, Article 54, 2019

  • Whitear M (1960) Chordotonal organs in Crustacea. Nature, London 187:522–523

    CAS  Google Scholar 

  • Whitear M (1962) The fine structure of crustacean proprioceptors. I. The chordotonal organs in the legs of the shore crab, Carcinus meanas. Philos TransR Soc B 245:291–325

    Google Scholar 

  • Whitear M (1965) The fine structure of crustacean proprioceptors. II. The thoracico- -coxal organs in Carcinus, Pagurus and Astacus. Philos TransR Soc B 248(752):437–462

    Google Scholar 

  • Wiersma CAG (1959) Movement receptors in decapod Crustacea. J Marine Biol Assoc UK 38:143–152

    Google Scholar 

  • Wiersma CAG, Boettiger EG (1959) Unidirectional movement fibers from a proprioceptive organ of the crab, Carcinus maenas. J Exp Biol 36:102–112

    Google Scholar 

  • Wilkens JL, Mercier AJ, Evans J (1985) Cardiac and ventilatory responses to stress and to neurohormonal modulators by the shore crab Carcinus maenas. Comp Biochem Physiol 82C:337–343

    CAS  Google Scholar 

  • Wycoff S, Weineck K, Conlin S, Grau E, Bradley A, Cantrell D, Eversole S, Grachen C, Hall K, Hawthorne D, Kinmon C, Ortiz Guerrero P, Patel B, Samuels K, Suryadevara C, Valdes G, Ray A, Fleckenstein L, Piana E, Cooper RL (2018) Investigating potential effects of clove oil (eugenol) in model crustaceans. Impluse, 1–21. https://impulse.appstate.edu/articles/2018/effects-clove-oil-eugenol-proprioceptive-neurons-heart-rate-and-behavior-model-crustac

  • Zakon HH (2012) Adaptive evolution of voltage-gated sodium channels: the first 800 million years. Proc Natl Acad Sci USA 109:10619–10625. https://doi.org/10.1073/pnas.1201884109

    Article  PubMed  Google Scholar 

  • Zhu YC, Yocom E, Sifers J, Uradu H, Cooper RL (2016a) Modulatory effects on Drosophila larva hearts: room temperature, acute and chronic cold stress. J Comp Physiol A 186:829–841

    CAS  Google Scholar 

  • Zhu YC, Uradu H, Majeed ZR, Cooper RL (2016b) Optogenetic stimulation of Drosophila heart rate at different temperatures and Ca2+ concentrations. Physiol Rep 4(3):e12695. https://doi.org/10.14814/phy2.12695

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

Funded by Dept. of Biology, Univ. of KY. and by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103436 (R.A.)

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  1. Department of Biology, University of Kentucky, Lexington, KY, 40506, USA

    Catherine E. Stanley, Ellora Amrit, Matthew Barrett, Catherine Bohnett, Kelsey Campbell, Keegan Deweese, Sabbyasachi Dhar, Barbara Gillis, Carson Hill, Morgan Inks, Katrina Kozak, Alexa Larson, Ibraheem Murtaza, Destaneh Nichols, Rafael Roberts, Hannah Tyger, Courtney Waterbury & Robin L. Cooper

  2. Department of Biological Sciences, Northern Kentucky University, Highland Heights, KY, 41099, USA

    Rebecca Adams

  3. Department of Mathematical and Computational Sciences, Benedictine University, Lisle, IL, 60532, USA

    Jeremy Nadolski

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Stanley, C.E., Adams, R., Nadolski, J. et al. The effects of tricaine mesylate on arthropods: crayfish, crab and Drosophila. Invert Neurosci 20, 10 (2020). https://doi.org/10.1007/s10158-020-00243-5

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