Elsevier

Theriogenology

Volume 154, 15 September 2020, Pages 128-134
Theriogenology

Effect of a puberty induction protocol based on injectable long acting progesterone on pregnancy success of beef heifers serviced by TAI

https://doi.org/10.1016/j.theriogenology.2020.05.036Get rights and content
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Highlights

  • A long-lasting source of P4 stimulates uterine and follicular development of prepubertal beef heifers.

  • An induction protocol based only on injectable hormones hastens puberty in beef heifers.

  • This induction protocol increased fertility of beef heifers submitted to timed-AI.

  • The protocol is a practical way to induce puberty in beef heifers.

Abstract

Induction protocols based on progesterone (P4) are used prior to a synchronization program for timed-AI (TAI) to increase number of pubertal heifers and pregnancy per AI (P/AI). Injectable, long-lasting P4 (iP4) is a novel, practical method to supplement P4 in cattle. Here, we aimed to test the effect of an induction protocol based on a single injection of iP4 on P/AI of heifers. Bos indicus (Nellore) heifers were classified as pubertal (PUB; n = 224) or prepubertal (PRE; n = 414) based on two ovarian ultrasonographyc exams conducted 10 d apart. Heifers with a corpus luteum (CL) in any of the exams were considered PUB. Within each puberty status, heifers were assigned to receive nothing (NoiP4) or an induction protocol (iP4). Induction consisted of a single injection of 150 mg of iP4 on D-31, followed by injections of 1 mg of estradiol benzoate (EB) and 150 μg of prostaglandin analogue (PGF) on D-21. On D-9, all heifers received 2 mg of EB + 75 μg of PGF associated to intravaginal P4-device insertion. On D-3, P4-releasing devices were removed and 150 μg of PGF injected. Heifers were inseminated based on estrus on D-1 or were TAI on D0. On D0, all heifers received a dose of GnRH analogue. On D-21, iP4 treatment stimulated a 50% increase in the uterine score (UTS) and a 19% increase in the diameter of the largest follicle of PRE heifers (P < 0.01). On D-9, PRE|iP4 group had a greater proportion (P < 0.01) of CL (63.3%) than PRE|NoiP4 group (11.6%). On D-3, exposure to 6 d P4-releasing device stimulated UTS of PRE|NoiP4 group in a similar fashion than the induction protocol, but it did not have any additional positive effect for PRE|iP4 heifers. P/AI of PRE|iP4 group was similar to that of the PUB groups (44.7 vs 46.9%), but was more than that of PRE|NoiP4 (34.2%). There was an overall 7.7% increment (P = 0.07) on P/AI of iP4 treated heifers (iP4: 46.0% vs. NoiP4: 38.3%). In conclusion, implementation of an induction protocol based on iP4 was efficacious to hasten puberty. Induction stimulated uterine development and follicular growth of prepubertal heifers, ultimately leading to pregnancy success similar to that of pubertal heifers.

Keywords

Puberty
Uterus
Follicle
Reproduction
Beef cattle

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