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Characterization of a new spiral nematode, Helicotylenchus asiaticus n. sp., and three known species from Korea; with comments on the validity of Helicotylenchus microlobus Perry in Perry, Darling & Thorne, 1959

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Abstract

A new spiral nematode Helicotylenchus asiaticus n. sp., recovered from sandy rhizosphere of Poa pratensis from Korea, is characterized based on integrative taxonomy, considering both morphological and molecular phylogenetic inferences from analyses of the 28S-rRNA and ITS-rRNA gene sequences. Additionally, H. caudatus is characterized for the first time outside its type locality; notes on the taxonomic status of H. microlobus are stated, and the existence of H. dihystera in Korea is molecularly confirmed. The new species is characterized by a lip region not offset; hemispherical, bearing 4–5 annuli; stylet knobs sloping posteriorly, rarely anteriorly flattened; spermatheca, offset and filled with sperms; incisures of the lateral field areolated in pharyngeal region, with incomplete areolation in the tail region; inner incisures usually distally modified into a u-shape or y-shaped configuration; phasmids postanal, situated at 3–8 annuli posterior to anus level; tail conical, bearing 8–15 annuli, ending in a rounded to pointed extremity; and males abundant. The new species is morphologically similar to H. erythrinae, H. californicus, H. electropicus, H. tropicus, H. conicus, H. elegans, H. interrogativus, H. saxeus, H. certus and H. silvaticus but differs from all, either by lip region shape, stylet knob shape, sperm filled spermatheca, phasmid position, tail termini shape and (or) presence of males. Sequence data analyses from amplified regions suggest that Helicotylenchus asiaticus n. sp. is a sister species to unidentified Helicotylenchus sp. From Korea.

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Acknowledgements

The authors thank Dr. Sergei A. Subbotin (California Department of Food and Agriculture Plant Pest Diagnostic Center, ca., USA) for the technical advices during the manuscript preparation. The authors also acknowledge the respective golf course management teams for the cooperation during the survey.

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  1. Department of Ecological Science, Kyungpook National University, Sangju, 37224, South Korea

    Abraham Okki Mwamula, Heebeen Na, Yeong Ho Kim, Young Ho Kim & Dong Woon Lee

  2. Department of Zoology, Entomology and Fisheries Sciences, College of Natural Sciences, Makerere University, Kampala, 7062, Uganda

    Abraham Okki Mwamula

  3. Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, South Korea

    Garam Han

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Mwamula, A.O., Na, H., Kim, Y.H. et al. Characterization of a new spiral nematode, Helicotylenchus asiaticus n. sp., and three known species from Korea; with comments on the validity of Helicotylenchus microlobus Perry in Perry, Darling & Thorne, 1959. Eur J Plant Pathol 157, 565–581 (2020). https://doi.org/10.1007/s10658-020-02022-9

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