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Enhancing the Stability of Haemonchus contortus Glyceraldehyde-3-Phosphate Dehydrogenase and Binding of Host Albumin to the Parasite Enzyme

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Abstract

Introduction

Haemonchus contortus is an economically important parasite of domestic animals. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) excreted in the ES product of H. contortus can be a promising vaccine candidate for controlling the parasite infection. Unfortunately, the parasite enzyme breaks down rapidly. The current study focusses on stabilizing the recombinant GAPDH (rGAPDH) of H. contortus.

Methods

The rGAPDH was purified and stored in two different buffers (sodium phosphate + EDTA and bicarbonate-sodium chloride) to check the stability. The affinity of the parasite enzyme towards host serum (Goat) components was evaluated by affinity chromatography. The interacting component was identified by mass spectrometry.

Results

Here, we report that the enzyme can be stabilized for at least 3 months if stored in bicarbonate-sodium chloride. This should facilitate testing of the enzyme in challenge trials. Additionally, we show that the parasite enzyme has affinity for host albumin; this interaction may have significance in host–parasite relationship.

Conclusion

The present study reports a combination of sodium bicarbonate (0.1 M) with 0.5 M sodium chloride as a suitable buffer to enhance the stability of H. contortus GAPDH.

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References

  1. Gilleard JS (2013) Haemonchus contortus as a paradigm and model to study anthelmintic drug resistance. Parasitol 140:1506–1522

    Article  CAS  Google Scholar 

  2. Bassetto CC, Amarante AFT (2015) Vaccination of sheep and cattle against haemonchosis. J Helminthol 89:517–525

    Article  CAS  PubMed  Google Scholar 

  3. Newton SA, Munn EA (1999) The development of vaccines against gastrointestinal nematode parasites, particularly Haemonchus contortus. Parasitol Today 15:116–122

    Article  CAS  PubMed  Google Scholar 

  4. Nisbet AJ, Meeusen EN, González JF, Piedrafita DM (2016) Chapter eight-immunity to Haemonchus contortus and vaccine development. Advances in parasitology, 1st edn. Academic Press, Berlin

    Google Scholar 

  5. Sahoo S, Murugavel S, Devi IK, Vedamurthy GV, Gupta SC, Singh BP, Joshi P (2013) Glyceraldehyde-3-phosphate dehydrogenase of the parasitic nematode Haemonchus contortus binds to complement C3 and inhibits its activity. Parasite Immunol 35:457–467

    Article  CAS  PubMed  Google Scholar 

  6. Han K, Xu L, Yan R, Song X, Li X (2012) Vaccination of goats with glyceraldehydes-3-phosphate dehydrogenase DNA vaccine induced partial protection against Haemonchus contortus. Vet Immunol Immunopathol 149:177–185

    Article  CAS  PubMed  Google Scholar 

  7. Vedamurthy GV, Sahoo S, Devi IK, Murugavel S, Joshi P (2015) The N-terminal segment of glyceraldehyde-3-phosphate dehydrogenase of Haemonchus contortus interacts with complements C1q and C3. Parasite Immunol 37:568–578

    Article  CAS  PubMed  Google Scholar 

  8. de Vries E, Bakker N, Kriejgsveld J, Knox DP, Heck AJR, Yatsuda AP (2009) An AC-5 cathepsin B-like protease purified from Haemonchus contortus excretory secretory products shows protective antigen potential for lambs. Vet Res. https://doi.org/10.1051/vetres/2009025

    Article  PubMed  PubMed Central  Google Scholar 

  9. Rajan P, Mishra PKK, Joshi P (2019) Defining the complement C3 binding site and the antigenic region of Haemonchus contortus GAPDH. Parasite Immunol. https://doi.org/10.1111/pim12611

    Article  PubMed  Google Scholar 

  10. Gozalbo D, Navarro G, Azorin I, Renau-Piqueras J, Martinez JP, Gil ML (1998) The cell wall associated glyceraldehyde-3-phosphate dehydrogenase of Candida albicans is also a fibronectin and laminin binding protein. Infection Immunity 66:2052–2059

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Terrasse R, Delorme PT, Moriscot C, Perard J, Schoehn G, Thiery V, Thielens NM, Guilmi AM, Frachet P (2012) Human and Pneumococcal cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH) proteins are both ligands of human C1q protein. J Biol Chem 287:42620–42633

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Quessy S, Busque P, Higgins R, Jacques M, Dubreuil JD (1997) Description of an albumin binding activity for Streptococcus suis serotype 2. FEMS Microbiol Lett 147:245–250

    Article  CAS  PubMed  Google Scholar 

  13. Gimenez R, Anguilera L, Ferreira E, Anguilar J, Baltma L, Badia J (2014) Glyceraldehyde-3-phosphate dehydrogenase as a moonlighting protein in bacteria. Recent Adv Pharmaceut Sciences IV:165–180

    Google Scholar 

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Acknowledgements

This work was supported by a Grant from the Department of Biotechnology, New Delhi, to P. J. YN was supported by IVRI and PKKM was recipient of ICMR fellowship. We thank Director, IVRI for providing the facilities.

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Authors and Affiliations

  1. Division of Biochemistry, ICAR-IVRI, Izatnagar, 243122, India

    Yapu Nijo & Paritosh Joshi

  2. Unit of Veterinary Clinical Complex, FVAS, RGSC, Banaras Hindu University, Varanasi, 231001, India

    Prasanta Kumar K. Mishra

Authors
  1. Yapu Nijo
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  2. Prasanta Kumar K. Mishra
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  3. Paritosh Joshi
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Contributions

PJ conceived the idea, partly executed, and wrote the manuscript. YN was involved in protein purification, stability testing, and analyzing GAPDH–albumin interactions, figure preparation, and reviewed the manuscript. PKKM was involved in blood collection, generation of affinity column, etc., and checked the draft.

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Correspondence to Prasanta Kumar K. Mishra.

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Nijo, Y., Mishra, P.K.K. & Joshi, P. Enhancing the Stability of Haemonchus contortus Glyceraldehyde-3-Phosphate Dehydrogenase and Binding of Host Albumin to the Parasite Enzyme. Acta Parasit. 65, 980–984 (2020). https://doi.org/10.2478/s11686-020-00212-3

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  • DOI: https://doi.org/10.2478/s11686-020-00212-3

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