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RHPN1-AS1 promotes cell proliferation and migration via miR-665/Akt3 in ovarian cancer

Cancer Gene Therapy volume 28, pages 33–41 (2021)Cite this article

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Abstract

Recent efforts have revealed that long non-coding RNAs exert crucial roles in cancer initiation and progression. RHPN1-AS1 is a 2030 bp transcript from human chromosome 8q24, and involved in tumorigenesis in uveal melanoma and non-small cell lung cancer, but it remains unknown in ovarian cancer. This study focused on the role of RHPN1-AS1 in ovarian cancer and found that RHPN1-AS1 was up-regulated in ovarian cancer tissues and cell lines. Overexpression of RHPN1-AS1 promoted ovarian cancer cell proliferation, migration, and invasion. Mechanistically, overexpression of RHPN1-AS1 decreased the expression of miR-665 and subsequently promoted the expression of Akt3 at posttranscriptional level. Taken together, RHPN1-AS1 positively regulated the expression of Akt3 through sponging miR-665, and exerted an oncogenic role in ovarian cancer progression, and indicates that RHPN1-AS1 may be a potential therapeutic target in ovarian cancer.

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Fig. 1: Up-regulated expression of RHPN1-AS1 in ovarian cancer tissue and cell lines.
Fig. 2: Effects of RHPN1-AS1 on ovarian cancer cell proliferation, migration, and invasion.
Fig. 3: RHPN1-AS1 interacts with miR-665.
Fig. 4: RHPN1-AS1 exerted roles through regulating miR-665 level.
Fig. 5: RHPN1-AS1 up-regulates Akt3 expression by competitively binding to miR-665.
Fig. 6: Knockdown of RHPN1-AS1 impaired ovarian cancer cell growth in vivo.

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Funding

This study was supported by Shaanxi Provincial Key R&D Program: General Project—Social Development Area, Fund number: 2017SF-016.

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Authors and Affiliations

  1. Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Xi’ an Jiaotong University, 710061, Xi’ an, China

    Juan Zhao, Ting Yang, Jing Ji, Fan Zhao, Chen Li & Xiaobing Han

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  1. Juan Zhao
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  4. Fan Zhao
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Contributions

XH and JZ conceived the experiments; JZ, TY, and CL performed the experiments; JZ and JJ analyzed the data; XH and JZ wrote the manuscript. All authors read and approved the final version of manuscript.

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Correspondence to Xiaobing Han.

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Zhao, J., Yang, T., Ji, J. et al. RHPN1-AS1 promotes cell proliferation and migration via miR-665/Akt3 in ovarian cancer. Cancer Gene Ther 28, 33–41 (2021). https://doi.org/10.1038/s41417-020-0180-0

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