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Disruption in phosphate transport affects membrane lipid and lipid droplet homeostasis in Saccharomyces cerevisiae

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Abstract

Phosphate plays a crucial role in phospholipid metabolism and it is transported by the phosphate (Pi) transporters. Phospholipids are building blocks of the cell membrane, and essential for cell growth; however, the role of phosphate transporters in lipid metabolism remains elusive. The present study shows that the deletion of Pi transporters exhibited an increase in both phospholipid and neutral lipid levels when compared to wild type. The mRNA expressions of genes involved in phospholipid synthesis (CKI1, EKI1, CHO2, and OPI3) were increased due to de-repression of the transcription factors (INO2 and INO4). Neutral lipid levels (triacylglycerol and sterol ester) and their synthesizing genes (LRO1, ARE2, ACC1, and FAS1) were also increased, resulting in lipid droplet accumulation in Pi transporter mutants. Interestingly, phospholipase (PLC1) and histone acetyltransferase genes (ESA1, EAF1, YNG1, YNG2, and GCN5) were also found to be significantly increased, leading to dysregulation of lipid levels in Pi transporter mutants. In summary, our results suggest that the Pi transporters are involved in lipid droplet and membrane lipid homeostasis.

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Acknowledgments

We thank Prof. Ram Rajasekharan, Central Food Technological Research Institute, India, for providing the yeast strains and reagents for this study. Junior Research Fellowship provided by the Department of Science & Technology (DST-PURSE), India, is gratefully acknowledged. Instrumentation facility under the DST-FIST program for the Department of Biochemistry, and DST-PURSE program and Centre for Excellence of the Bharathidasan University are gratefully acknowledged.

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  1. Mani Subitha and Antonisamy William James contributed equally to this work.

Authors and Affiliations

  1. Biomembrane Lab, Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620 024, India

    Mani Subitha, Antonisamy William James, Chinnarasu Sivaprakasam & Vasanthi Nachiappan

  2. Department of Medicine and Cancer Biology, The University of Toledo, Toledo, OH, 43614, USA

    Antonisamy William James

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  1. Mani Subitha
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Correspondence to Vasanthi Nachiappan.

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Subitha, M., James, A.W., Sivaprakasam, C. et al. Disruption in phosphate transport affects membrane lipid and lipid droplet homeostasis in Saccharomyces cerevisiae. J Bioenerg Biomembr 52, 215–227 (2020). https://doi.org/10.1007/s10863-020-09837-5

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