Abstract
Phellodendron chinense Schneid is an important Chinese herb with berberine and phellodendrine in stems and leaves, but with little information available on in vitro culture of this species. Disinfection of explants in 75% alcohol for 45 s, sterilization in 0.1% HgCl2 for 20 min, and submersion in 1.0 mol L−1 gibberellin3 (GA3) solution for 24 h was the optimal condition for seed germination. Murashige and Skoog’s (MS) medium supplemented with 2.0 mg L−1 6-benzylaminopurine (6-BA) in combination with 1.5 mg L−1 1-naphthylacetic acid (NAA) was optimal for callus induction. MS medium supplemented with 2.0 mg L−1 6-BA was the appropriate medium for induction of adventitious shoots, and 1/2MS medium supplemented with 2.0 mg L−1 indole-3-butytric acid (IBA) and 0.5% active carbon was the optimal medium for root induction. The 15-d survival rate of regenerated plantlets after transplanting to basins containing perlite and peat moss (1:4) was greater than 80%, and the berberine and phellodendrine accumulation was lower in callus compared with regenerated plantlets. The establishment of highly efficient regeneration system provides technical support for genetic breeding of Phellodendron chinense Schneid.
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References
Azad MAK, Yokota S, Begum F, Yoshizawa N (2009) Plant regeneration through somatic embryogenesis of a medicinal plant, Phellodendron amurense Rupr. In Vitro Cell Dev-Plant 45:441–449. https://doi.org/10.1007/s11627-008-9171-9
Benitez-Rodriguez L, Gamboa-deBuen A, Sanchez-Coronado ME, Alvarado-Lopez S, Soriano D, Mendez I, Vazquez-Santana S, Carabias-Lillo J, Mendoza A, Orozco-Segovia A (2014) Effects of seed burial on germination, protein mobilisation and seedling survival in Dodonaea viscosa. Plant Biol (Stuttg) 16:732–739. https://doi.org/10.1111/plb.12110
Choudhary R, Chaudhury R, Malik SK, Sharma KC (2015) An efficient regeneration and rapid micropropagation protocol for almond using dormant axillary buds as explants. Indian J Exp Biol 53:462–467
Cui Y, Deng Y, Zheng K, Hu X, Zhu M, Deng X, Xi R (2019) An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers. Sci Rep 9:9634. https://doi.org/10.1038/s41598-019-46050-w
Fang S, Gao K, Hu W, Snider JL, Wang SS, Chen BL, Zhou ZG (2018) Chemical priming of seed alters cotton floral bud differentiation by inducing changes in hormones, metabolites and gene expression. Plant Physiol Biochem 130:633–640. https://doi.org/10.1016/j.plaphy.2018.08.010
Gao X, Wang J, Li M, Wang J, Lv J, Zhang L, Sun C, Ji J, Yang W, Zhao Z, Mao W (2019) Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs. J Cell Mol Med 23:6797–6804. https://doi.org/10.1111/jcmm.14560
He H, Qin J, Cheng X, Xu K, Teng L, Zhang D (2018) Effects of exogenous 6-BA and NAA on growth and contents of medicinal ingredient of Phellodendron chinense seedlings. Saudi J Biol Sci 25:1189–1195. https://doi.org/10.1016/j.sjbs.2017.11.037
Ikeuchi M, Sugimoto K, Iwase A (2013) Plant callus: mechanisms of induction and repression. Plant Cell 25:3159–3173. https://doi.org/10.1105/tpc.113.116053
Manoj P, Kumar SS, Giridhar P (2019) In vitro shoot multiplication of Rumex vesicarius L., and quantification of ascorbic acid and major phenolics from its leaf derived callus. 3 Biotech 9:353. https://doi.org/10.1007/s13205-019-1877-3
Martinez-de la Cruz E, Garcia-Ramirez E, Vazquez-Ramos JM, de la Cruz HR, Lopez-Bucio J (2015) Auxins differentially regulate root system architecture and cell cycle protein levels in maize seedlings. J Plant Physiol 176:147–156. https://doi.org/10.1016/j.jplph.2014.11.012
Mosca G, Sapala A, Strauss S, Routier-Kierzkowska AL, Smith RS (2017) On the micro-indentation of plant cells in a tissue context. Phys Biol 14:015003. https://doi.org/10.1088/1478-3975/aa5698
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Penfield S (2017) Seed dormancy and germination. Curr Biol 27:R874–R878. https://doi.org/10.1016/j.cub.2017.05.050
Perveen S, Bin Javed S, Anis M, Aref IM (2013) Rapid in vitro multiplication and ex vitro establishment of Caribbean copper plant (Euphorbia cotinifolia L.): an important medicinal shrub. Acta Physiol Plant 35:3391–3400. https://doi.org/10.1007/s11738-013-1374-y
Rashmi R, Trivedi MP (2014) Effect of various growth hormone concentration and combination on callus induction, nature of callus and callogenic response of Nerium odorum. Appl Biochem Biotechnol 172:2562–2570. https://doi.org/10.1007/s12010-013-0693-1
Ren JW, Lei Y, Li XL (2017) Tissue culture of callus and establishment of regeneration system of Tussilago farfara petiole. Zhongguo Zhong Yao Za Zhi 42:3895–3900. https://doi.org/10.19540/j.cnki.cjcmm.2017.0156
Song Q, Cheng S, Chen Z, Nie G, Xu F, Zhang J, Zhou M, Zhang W, Liao Y, Ye J (2019) Comparative transcriptome analysis revealing the potential mechanism of seed germination stimulated by exogenous gibberellin in Fraxinus hupehensis. BMC Plant Biol 19:199. https://doi.org/10.1186/s12870-019-1801-3
Steinbrecher T, Leubner-Metzger G (2017) The biomechanics of seed germination. J Exp Bot 68:765–783. https://doi.org/10.1093/jxb/erw428
Sun Y, Lenon GB, Yang AWH (2019) Phellodendri cortex: a phytochemical, pharmacological, and pharmacokinetic review. Evid Based Complement Alternat Med 2019:7621929. https://doi.org/10.1155/2019/7621929
Takahara M, Takaki A, Hiraoka S, Adachi T, Shimomura Y, Matsushita H, Nguyen TTT, Koike K, Ikeda A, Takashima S, Yamasaki Y, Inokuchi T, Kinugasa H, Sugihara Y, Harada K, Eikawa S, Morita H, Udono H, Okada H (2019) Berberine improved experimental chronic colitis by regulating interferon-gamma- and IL-17A-producing lamina propria CD4+ T cells through AMPK activation. Sci Rep 9:11934. https://doi.org/10.1038/s41598-019-48331-w
Tan J, Wang J, Yang C, Zhu C, Guo G, Tang J, Shen H (2019) Antimicrobial characteristics of Berberine against prosthetic joint infection-related Staphylococcus aureus of different multi-locus sequence types. BMC Complement Altern Med 19:218. https://doi.org/10.1186/s12906-019-2558-9
Tan M, Li G, Qi S, Liu X, Chen X, Ma J, Zhang D, Han M (2018) Identification and expression analysis of the IPT and CKX gene families during axillary bud outgrowth in apple (Malus domestica Borkh.). Gene 651:106–117. https://doi.org/10.1016/j.gene.2018.01.101
Teardo E, Carraretto L, Moscatiello R, Cortese E, Vicario M, Festa M, Maso L, De Bortoli S, Cali T, Vothknecht UC, Formentin E, Cendron L, Navazio L, Szabo I (2019) A chloroplast-localized mitochondrial calcium uniporter transduces osmotic stress in Arabidopsis. Nat Plants 5:581–588. https://doi.org/10.1038/s41477-019-0434-8
Wang L, Du Y, Rahman MM, Tang B, Fan LJ, Kilaru A (2018) Establishment of an efficient in vitro propagation system for Iris sanguinea. Sci Rep-Uk 8:17100. https://doi.org/10.1038/s41598-018-35281-y
Wang PF, Li YP, Ding LQ, Cao SJ, Wang LN, Qiu F (2019) Six new methyl apiofuranosides from the bark of Phellodendron chinense Schneid and their inhibitory effects on nitric oxide production. Molecules 24:1851. https://doi.org/10.3390/molecules24101851
Wang Y, Tong Q, Shou JW, Zhao ZX, Li XY, Zhang XF, Ma SR, He CY, Lin Y, Wen BY, Guo F, Fu J, Jiang JD (2017) Gut microbiota-mediated personalized treatment of hyperlipidemia using berberine. Theranostics 7:2443–2451. https://doi.org/10.7150/thno.18290
Wang YH (2006) Study on establishment of system with high frequency for genetic transformation of Phellodendron chinese and plant regeneration. Zhong Yao Cai 29:641–644
Xu X, Zhu XP, Bai JY, Xia P, Li Y, Lu Y, Li XY, Gao X (2019) Berberine alleviates nonalcoholic fatty liver induced by a high-fat diet in mice by activating SIRT3. FASEB J 33:7289–7300. https://doi.org/10.1096/fj.201802316R
Yu ZC, Cen YX, Wu BH, Wei C, Xiong F, Li DF, Liu TT, Luo MH, Guo LL, Li YX, Wang LS, Wang JY, Yao J (2019) Berberine prevents stress-induced gut inflammation and visceral hypersensitivity and reduces intestinal motility in rats. World J Gastroenterol 25:3956–3971. https://doi.org/10.3748/wjg.v25.i29.3956
Zhang CL, Yang DF, Liang ZS, Liu JL, Yan KJ, Zhu YH, Yang SS (2019) Climatic factors control the geospatial distribution of active ingredients in Salvia miltiorrhiza Bunge in China. Sci Rep-Uk 9:904. https://doi.org/10.1038/s41598-018-36729-x
Zhang Z, Zhao H, Li W, Wu J, Zhou Z, Zhou F, Chen H, Lin Y (2018) Genome-wide association study of callus induction variation to explore the callus formation mechanism of rice. J Integr Plant Biol 61:1134–1150. https://doi.org/10.1111/jipb.12759
Zhao L, Liu S, Wang M, Zhi M, Geng X, Hou C, Wang W, Zhao D (2019) Berberine restored nitrergic and adrenergic function in mesenteric and iliac arteries from streptozotocin-induced diabetic rats. J Ethnopharmacol 244:112140. https://doi.org/10.1016/j.jep.2019.112140
Zhu SL, Dou SS, Liu XR, Liu RH, Zhang WD, Huang HL, Zhang Y, Hu YH, Wang SP (2011) Qualitative and quantitative analysis of alkaloids in cortex phellodendri by HPLC-ESI-MS/MS and HPLC-DAD. Chem Res Chinese U 27:38–44. https://doi.org/10.1016/j.cnki:sun:ghyj.0.2011-01-010
Zhu X, Bian H, Wang L, Sun X, Xu X, Yan H, Xia M, Chang X, Lu Y, Li Y, Xia P, Li X, Gao X (2019) Berberine attenuates nonalcoholic hepatic steatosis through the AMPK-SREBP-1c-SCD1 pathway. Free Radic Biol Med 141:192–204. https://doi.org/10.1016/j.freeradbiomed.2019.06.019
Funding
This research was financially supported by the Key Research and Development Project of Hunan Province (No. 2018NK2041), Key Project of Hunan Education Department (No. 18A158; 19B590), and Changsha Science and Technology Project (No. kq1901144). These funding bodies had no influence on the design of the study, collection, analysis, interpretation of data, or the manuscript writing.
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He, H., Qin, J., Ma, Z. et al. Highly efficient regeneration and medicinal component determination of Phellodendron chinense Schneid. In Vitro Cell.Dev.Biol.-Plant 56, 775–783 (2020). https://doi.org/10.1007/s11627-020-10080-1
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DOI: https://doi.org/10.1007/s11627-020-10080-1