Abstract
In recent years the search for beneficial microorganisms that could induce plant growth has increased. Most have been found on crop seeds, soil and on the rhizosphere. In this work, we collected mature seeds of Phaseolus vulgaris var. Pinto Saltillo in Zacatecas, Mexico; and isolated endophytic fungi from these samples. Three different fungal strains were obtained. Sequencing analysis showed that one of the analyzed fungi, Alternaria solani IA300, showed the capacity to promote plant growth. Chili plants (Capsicum annuum cv. Mirasol) were inoculated with Alternaria solani IA300. After 15, 30, 45, and 60 days post-interaction, nine vegetative variables were analyzed (length of the root and aerial parts; weight of the root and aerial parts; number of leaves, flowers and fruits; as well as the dry weight from the root and aerial parts). Results showed that Alternaria solani IA300 can promote growth in chili pepper plants at later times of interaction (45 and 60 days post interaction), where most of the vegetative variables analyzed showed an increase in relation to control plants. To our knowledge, this is the first report where Alternaria solani IA300 induces growth and development in plants.
References
Abdel-Hafez, S. I., Nafady, N. A., Abdel-Rahim, I. R., Shaltout, A. M., Daròs, J. A., & Mohamed, M. A. (2016). Assessment of protein silver nanoparticles toxicity against pathogenic Alternaria solani. 3 Biotech, 6(2), 199.
Avis, T. J., Gravel, V., Antoun, H., & Tweddell, R. J. (2008). Multifaceted beneficial effects of rhizosphere microorganisms on plant health and productivity. Soil biology and biochemistry, 40(7), 1733–1740.
Gopalakrishnan, S., Kiran, B. K., Humayun, P., Vidya, M. S., Deepthi, K., Jacob, S., & Rupela, O. (2011). Biocontrol of charcoal-rot of sorghum by actinomycetes isolated from herbal vermicompost. African Journal of Biotechnology, 10(79), 18142–18152
Herrera, S. D., Grossi, C., Zawoznik, M., & Groppa, M. D. (2016). Wheat seeds harbor bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum. Microbiological Research, 186, 37–43.
Kumar, M., Bhadauria, V., Singh, K., Singh, C., & Yadav, A. K. (2013). Evaluation of fungicide efficacy for the Management of Alternaria Leaf Spot Disease on chili. Plant Pathology Journal, 12(1), 32–35.
Lara-Flores, M. (2015). El cultivo del frijol en México. Revista Digital Universitaria de la UNAM. 16(2), 1–11.
Li, Y., Zhang, D., Xu, W., Wu, Z., Guo, M., & Cao, A. (2011). Alternaria tenuissima causing leaf spot and fruit rot on pepper (Capsicum annuum): First report in China. New Dis Rep, 24(3).
López-López, A., Rogel, M. A., Ormeno-Orrillo, E., Martínez-Romero, J., & Martínez-Romero, E. (2010). Phaseolus vulgaris seed-borne endophytic community with novel bacterial species such as Rhizobium endophyticum sp. nov. Systematic and Applied Microbiology, 33(6), 322–327.
Mendes, R., Garbeva, P., & Raaijmakers, J. M. (2013). The rhizosphere microbiome: significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms. FEMS microbiology reviews, 37(5), 634–663.
Parsa, S., García-Lemos, A. M., Castillo, K., Ortiz, V., López-Lavalle, L. A. B., Braun, J., & Vega, F. E. (2016). Fungal endophytes in germinated seeds of the common bean, Phaseolus vulgaris. Fungal Biology, 120(5), 783–790.
Raeder, U., & Broda, P. (1985). Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology, 1(1), 17–20.
Rijavec, T., Lapanje, A., Dermastia, M., & Rupnik, M. (2007). Isolation of bacterial endophytes from germinated maize kernels. Canadian Journal of Microbiology, 53(6), 802–808.
Robles-Yerena, L., Rodríguez-Villarreal, R. A., Ortega-Amaro, M. A., Fraire-Velázquez, S., Simpson, J., Rodríguez-Guerra, R., & Jiménez-Bremont, J. F. (2010). Characterization of a new fungal antagonist of Phytophthora capsici. Scientia horticulturae, 125(3), 248–255.
SIAP, Servicio de Información Agroalimentaria y Pesquera (2018) Anuario Estadístico de la Producción Agrícola. Servicio de Información Agroalimentaria y Pesquera. Secretaria de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación. México. http://nube.siap.gob.m x/cierre_agricola/ (Abril 2018).
Singh, S. P. (1999). Production and utilization. In S. P. Singh (Ed.), Common bean improvement in the twenty-first century (p. 1e24). Dordrecht: Kluwer Academic Publishers.
Song, W., Ma, X., Tan, H., & Zhou, J. (2011). Abscisic acid enhances resistance to Alternaria solani in tomato seedlings. Plant Physiology and Biochemistry, 49(7), 693–700.
Spletzer, M. E., & Enyedi, A. J. (1999). Salicylic acid induces resistance to Alternaria solani in hydroponically grown tomato. Phytopathology, 89(9), 722–727.
Sreenivasa, M. N., Naik, N., & Bhat, S. N. (2010). Beejamrutha: A source for beneficial bacteria. Karnataka Journal of Agricultural Sciences, 22(5).
Van Aken, B., Peres, C. M., Doty, S. L., Yoon, J. M., & Schnoor, J. L. (2004). Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (Populus deltoides× nigra DN34). International journal of systematic and evolutionary microbiology, 54(4), 1191–1196.
Vassilev, N., Vassileva, M., & Nikolaeva, I. (2006). Simultaneous P-solubilizing and biocontrol activity of microorganisms: potentials and future trends. Applied microbiology and biotechnology, 71(2), 137–144.
Vessey, J. K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255, 571–586.
Zhou, L., Tang, K., & Guo, S. (2018). The plant growthpromoting fungus (PGPF) Alternaria sp. A13 markedly enhances Salvia miltiorrhiza root growth and active ingredient accumulation under greenhouse and field conditions. International Journal of Molecular Science 19, 270. https://doi.org/10.3390/ijms19010270.
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Funding: FBSB received grant (UAZ-PTC-214) from PRODEP.
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S.S.M., J.A.M.C., L.R.R.T., M.A.S.L. and F.B.S.B. conceived the experimental design. S.S.M., L.R.R.T. and F.B.S.B. contributed with reagents, materials and analysis tools. S.S.M., J.A.M.C., L.R.R.T., M.A.S.L. and F.B.S.B. conducted the experiments and analyzed the data. S.S.M., J.A.M.C., L.R.R.T., M.A.S.L. and F.B.S.B. contributed to data interpretation and manuscript preparation. All authors read and approved the manuscript.
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Mauricio-Castillo, J.A., Salas-Muñoz, S., Reveles-Torres, L.R. et al. Could Alternaria solani IA300 be a plant growth-promoting fungus?. Eur J Plant Pathol 157, 413–419 (2020). https://doi.org/10.1007/s10658-020-01984-0
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DOI: https://doi.org/10.1007/s10658-020-01984-0