Abstract
Genetic resources are the fundamental source of diversity to plant breeders for improvement of desired traits. However, large germplasm set is difficult to preserve and use as working collection in genetic studies, hence in the present study evaluation of genetic diversity of 604 durum germplasm originated from different geographical areas and development of core collection for representation of diverse germplasm for working collection was done. Six hundred and four durum germplasm were sown in augmented design and data were recorded for eight quantitative characters including agronomic and grain quality traits. Descriptive statistics showed large variation for all studied traits. Box plot analysis for nine different sets of germplasm showed a large variation for agronomic traits and quality traits. Principal component analysis for the first four principal components explained 71% of the cumulative variation and grouped germplasm in the main two groups. The core germplasm set was developed using corehunter package with 10% cutoff and hence 60 germplasm entries make representation in core subset based on maximum diversity and minimum redundancy. The maximum contribution to core germplasm was by each CZ (Central Zone) breeding lines and D numbers (23%) followed by exotic other lines (20%). The developed core subset was validated using multivariate analysis such as Shannon diversity index, comparison of means and homogeneity of error variances using the Levene’s test. The results described in this study will be useful for durum wheat breeders for the development of varieties with high end-use quality.
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Balfourier F, Balfourier F, Roussel V, Strelchenko P, Exbrayat-Vinson F, Sourdille P, Boutet G, Koenig J, Ravel C, Mitrofanova O, Beckert M, Charmet G (2007) A worldwide bread wheat core collection arrayed in a 384-well plate. Theor Appl Genet 114:1265–1275
Balyan HS, Gupta PK, Mir RR, Kumar J (2009) Genetic diversity and population structure analysis among Indian bread wheat varieties. In: Appels R, Eastwood R, Lagudah E, Langridge P, Mackay M, McIntyre L, Sharp P (eds) Proceedings of the 11th international wheat genetic symposium, Brisbane, pp 185–187
Brown ADH (1989) Core collections: a practical approach to genetic resources management. Genome 31:818–824
Brown AHD (1995) The core collection at the crossroads. In: Hodgkin T, Brown AHD, van Hintum TJL, Morales EAV (eds) Core collections of plant genetic resources. International Plant Genetic Resources Institute (IPGRI), Wiley, New York, pp 3–20
Dick JW, Matsuo RR (1988) Durum wheat and pasta products. In: Pomeranz Y (ed) Wheat chemistry and technology, vol 2, 3rd edn. American Association of Cereal Chemists, St Paul
Dutta M, Phogat BS, Kumar S, Kumar N, Kumari J, Pandey AC, Singh TP, Tyagi RK, Jacob SR, Srinivasan K, Bisht IS, Karale M, Yadav M, Sharma P, Kumari G, Aftab T, Rathi YS, Singh AK, Archak S, Bhat KV, Bhandari DC, Solanki YPS, Singh D, Bansal KC (2015) Development of core set of wheat (Triticum spp.) germplasm conserved in the national genebank in India. In: Advances in wheat genetics: from genome to field. https://doi.org/10.1007/978-4-431-55675-6_4
Ferguson AR (2006) The need for characterization and evaluation of germplasm: kiwifruit as an example. Euphytica 154(3):371–382
Frankel OH, Brown AHD (1984) Plant genetic resources today: a critical appraisal. In: Hoden HW, Williams JT (eds) Crop genetic resources: conservation and evaluation. George Allen and Unwin, London, pp 249–257
Fu Y, Somers D (2009) Genome-wide reduction of genetic diversity in wheat breeding. Crop Sci 49:161–168
Joshi BK, Mudwari A, Bhatta MR, Ferrara GO (2004) Genetic diversity in Nepalese wheat cultivars based on agro morphological traits and coefficients of parentage. Nep Agric Res J 5:7–17
Levene H (1960) Robust tests for equality of variances. In: Olkin I (ed) Contributions to probability and statistics: essays in honour of Harold hotelling. Stanford University Press, Stanford, pp 278–292
Lopes MS, El-Basyoni I, Baenziger PS, Singh S, Royo C, Ozbek K, Aktas H, Ozer E, Ozdemir F, Manickavelu A, Ban T, Vikram P (2015) Exploiting genetic diversity from landraces in wheat breeding for adaptation to climate change. J Exp Bot 66(12):3477–3486. https://doi.org/10.1093/jxb/erv122
Mohammadi SA, Prasanna BM (2003) Analysis of genetics diversity in crop plants: salient statical tools and considerations. Crop Sci 43:1235–1248
Stehno Z, Faberova I, Dotlacil L, Martynov S, Dobrotvorskaya T (2006) Genealogical analysis in the Czech spring wheat collection and its use for the creation of core collection. Czech J Genet Plant Breed 42:117–125
Thachuk C, Crossa J, Franco J, Dreisigacker S, Warburton M, Davenport G (2009) Core Hunter: an algorithm for sampling genetic resources based on multiple genetic measures. BMC Bioinform 243(10):1471–2105. https://doi.org/10.1186/1471-2105-10-243
Upadhyaya HD (2003) Phenotypic diversity in groundnut (Arachis hypogaea L) core collection assessed by morphological and agronomical evaluations. Genet Resour Crop Evol 50:539–550
Upadhyaya HD, Ortiz R, Bramel PJ (2003) Development of a groundnut core collection using taxonomical, geographical and morphological descriptors. Genet Resour Crop Evol 50:139–148
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Ambati, D., Phuke, R.M., Vani, V. et al. Assessment of genetic diversity and development of core germplasm in durum wheat using agronomic and grain quality traits. CEREAL RESEARCH COMMUNICATIONS 48, 375–382 (2020). https://doi.org/10.1007/s42976-020-00050-z
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DOI: https://doi.org/10.1007/s42976-020-00050-z