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Age-Related Effects of Orthovanadate Nanoparticles Involve Activation of GSH-Dependent Antioxidant System in Liver Mitochondria

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Abstract

Vanadium is an important ultra-trace element nowadays attracting attention with particular emphasis on medical application. But the therapeutic application of vanadium-based drugs is still questionable and restricted due to some toxic side effects. It was found that unique redox properties of vanadium in nanoform provided antioxidant activity and prevented oxidative disturbance in cells in vitro. Though, on the organism level, ambiguous effects of vanadium-based nanoparticles were observed. In this study, the age-related features of prooxidant/antioxidant balance in blood serum and liver mitochondrial and postmitochondrial fractions of 3 and 18-month-old Wistar male rats treated with orthovanadate nanoparticles (GdVO4/Eu3+, 8 × 25 nm) within 2 months have been investigated. Prooxidant potential-related indexes were the content of lipid hydroperoxides as well as aconitase activity. Activity of glutathione peroxidase, glutathione-S-transferase, glutaredoxin, glutathione reductase, glucose-6-phosphate dehydrogenase, and NADPH-dependent isocitrate dehydrogenase designated the tissue antioxidant potential. Based on the obtained values, the integral index of the prooxidant/antioxidant balance—the reliability coefficient (Kr) has been calculated. The data show that due to activation some chain links of GSH-dependent antioxidant system, GdVO4/Eu3+ nanoparticles increase the reliability of the prooxidant-antioxidant balance in tissues and especially in the liver mitochondria of old animals (Kr in mitochondria of young rats was 2.94, and in mitochondria of old ones—9.83 conventional units). Detected in vitro glutathione peroxidase-like activity of the GdVO4/Eu3+ nanoparticles is supposed to be among factors increasing the reliability of the system. So, for the first time, the beneficial effect of the long-term orthovanadate nanoparticle consumption in old males has been discovered.

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  • 05 June 2020

    The original version of this article unfortunately contained a mistake.

Abbreviations

Aco:

Aconitase activity

cu:

Conventional units

DLS:

Dynamic light scattering

EDTA:

Ethylenediaminetetraacetic acid

G-6-PDG:

Glucose-6-phosphate dehydrogenase

GPx:

Glutathione peroxidase

GR:

Glutathione reductase

Grx:

Glutaredoxin

GSH:

Glutathione

GSSG:

Glutathione disulfide

GST:

Glutathione-S-transferase

ICDG:

Isocitrate dehydrogenase

Kr:

The integral index of the prooxidant-antioxidant balance

LHPO:

Lipid hydroperoxide

LPO:

Lipid peroxidation

MDA:

Malonic dialdehyde

MC:

Mitochondrial fraction of liver

NADP:

Nicotinamide adenine dinucleotide phosphate

NADPH:

Nicotinamide adenine dinucleotide phosphate+

NP:

Nanoparticle

PMC:

Postmitochondrial fraction of liver

ROS:

Reactive oxygen species

SEM:

Standard error of mean

TBA:

Thiobarbituric acid

Tris:

(Oxymethyl) aminomethane

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Funding

This work was supported by the State Fund For Fundamental Research (project no. Ф64/29-2016).

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Authors and Affiliations

  1. Institute for Scintillation Materials, National Academy of Sciences of Ukraine, 60 Nauky Ave., Kharkiv, 61072, Ukraine

    Vladimir K. Klochkov, Nataliya S. Kavok, Nina A. Karpenko & Svetlana L. Yefimova

  2. Biology Research Institute, Karazin Kharkiv National University, pl. Svobody 4, Kharkiv, 61000, Ukraine

    Yuri V. Nikitchenko, Irina V. Nikitchenko & Anatoly I. Bozhkov

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  1. Yuri V. Nikitchenko
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Correspondence to Nataliya S. Kavok.

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Nikitchenko, Y.V., Klochkov, V.K., Kavok, N.S. et al. Age-Related Effects of Orthovanadate Nanoparticles Involve Activation of GSH-Dependent Antioxidant System in Liver Mitochondria. Biol Trace Elem Res 199, 649–659 (2021). https://doi.org/10.1007/s12011-020-02196-7

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