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Elevated vitreous αB-crystallin in patients with rhegmatogenous retinal detachment and association with proliferative vitreoretinopathy and retinal detachment area

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Abstract

Purpose

To investigate the relationship between αB-crystallin levels in the vitreous of patients with rhegmatogenous retinal detachment (RRD) and correlate these levels with the presence of proliferative vitreoretinopathy (PVR) and to the extent of RRD.

Methods

Prospective, cross-sectional study. Vitreous samples were collected from twenty-six patients with RRD at the surgical repair. PVR was evaluated using the Updated Proliferative Vitreoretinopathy Grade Classification system. RRD area was measured using ultra-widefield retinal photographs. The concentration of αB-crystallin in the vitreous was measured using an enzyme-linked immunosorbent assay kit.

Results

The average concentration of αB-crystallin was significantly higher in the PVR Grade B and C group (97.83 ± 69.47) than in the PVR (-) and Grade A group (39.43 ± 13.53 ng/mL, P = 0.005). The level of αB-crystallin was also significantly higher in patients with large RD area (RD area > 1/4, 85.52 ± 66.07 ng/mL) than in patients with small RD area (RD area ≤ 1/4, 39.20 ± 13.02 ng/mL, P = 0.016).

Conclusions

The level of αB-crystallin was significantly increased in the vitreous of RRD patients with high-grade PVR and large RD area. This finding suggests that αB-crystallin may function as an endogenous mediator in fibrotic processes in RRD patients.

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Funding

This work was supported by the 2018 Ulsan University Hospital Research Grant (UUH-2018–05) and Chungbuk National University (2019).

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Authors and Affiliations

  1. College of Pharmacy, Chungbuk National University, Cheongju, Republic of Korea

    Jaesuk Yun & Jung Kee Min

  2. Department of Ophthalmology, Ulsan University Hospital, University of Ulsan, College of Medicine, 877, Bangeojinsunhwando-ro, Dong-gu, Ulsan, 44033, Republic of Korea

    Jung Kee Min

Authors
  1. Jaesuk Yun
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Correspondence to Jung Kee Min.

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The authors report no conflicts of interest.

Ethical approval

This study was reviewed and approved by the Institutional Human Experimentation Committee Review Board of Ulsan University Hospital, Ulsan, Korea (UUH 2018-03-006). The study was conducted in accordance with the ethical standards set forth in the 1964 Declaration of Helsinki.

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After a detailed explanation of study benefits and risks, informed consent was obtained from all patients.

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Yun, J., Min, J.K. Elevated vitreous αB-crystallin in patients with rhegmatogenous retinal detachment and association with proliferative vitreoretinopathy and retinal detachment area. Int Ophthalmol 40, 2461–2467 (2020). https://doi.org/10.1007/s10792-020-01425-1

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  • DOI: https://doi.org/10.1007/s10792-020-01425-1

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