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Plant Growth-Promoting Traits of a Thermophilic Strain of the Klebsiella Group with its Effect on Rice Plant Growth

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Abstract

In agriculture, instead of synthetic fertilizers, natural bio-inoculants can be used to increase growth and yield of crops. For this purpose, we report a thermophilic bacteria Klebsiella sp. strain PMnew, isolated from Paniphala hot spring. The strain was characterized and assessed for plant growth-promoting traits. Oryza sativa L. var Swarna (rice) seeds were inoculated with the strain to study the bacterization effect on vegetative and reproductive growth of rice plants. The results indicate that PMnew produces organic acids to solubilize phosphate (550.16 ± 0.04 µg/ml), fixes nitrogen, produces indole compounds, siderophore, and ACC deaminase, and shows heavy metal resistance to chromium, cobalt, arsenic, cadmium, and mercury. It also possesses the ability to utilize several monomeric and polymeric sugars as sole carbon source including starch, agar, xylan, gelatin, and pectin, and can grow under both nutrient-rich and deficient conditions. Inoculated rice plants grew twice the length of control plants and surpassed the total grain mass yield of control plants by almost 18 times. Thus, this study brings forth a broad spectrum and easy to cultivate bio-inoculant, which can be used to increase rice production.

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Acknowledgement

This study was funded by DST-INSPIRE (Innovation in Scientific Pursuit for Inspired Research, Department of Science and Technology) (IF 140017), Ministry of Science and Technology, Govt. of India, and UGC-sponsored Major Research Project Grant (MRP-MAJOR-MICR-2013-7783).

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Authors and Affiliations

  1. Department of Microbiology, The University of Burdwan, Burdwan, West Bengal, 713104, India

    Trinetra Mukherjee & Subhra Kanti Mukhopadhyay

  2. Department of Life Sciences, Presidency University, 86/1, College Street, Kolkata, West Bengal, 700073, India

    Avishek Banik

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  1. Trinetra Mukherjee
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  2. Avishek Banik
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  3. Subhra Kanti Mukhopadhyay
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TM, AB, and SKM conceived and designed the research. TM, AB, and SKM conducted the experiments and analyzed the data. TM, AB, and SKM wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Subhra Kanti Mukhopadhyay.

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Mukherjee, T., Banik, A. & Mukhopadhyay, S.K. Plant Growth-Promoting Traits of a Thermophilic Strain of the Klebsiella Group with its Effect on Rice Plant Growth. Curr Microbiol 77, 2613–2622 (2020). https://doi.org/10.1007/s00284-020-02032-0

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