Abstract
Gelatin-based nanocomposite films were prepared by casting and characterized as a sensitive hybrid material to relative humidity changes in the development of electrical sensors. Zinc oxide nanoparticles (ZnO-NPs) and glycerol (Gly) were incorporated as reinforcing and plasticizing agents, respectively, and their effects on the sensibility property of films were investigated. The proposed sensors were investigated at 25 °C. The behavior of gelatin-based films as a function of water presence was analyzed in terms of moisture content (MC), water vapor permeability (WVP), and water contact angle (WCA). The incorporation of ZnO-NPs and Gly induced morphological and structural changes in the composite films. Increased WCA (7.51%) and reduced WVP (18%) were observed for the nanocomposite films. In addition, ZnO-NPs and Gly significantly contributed to a further improvement of relative humidity sensibility of films compared to the control film. Electrical characterizations, carried out at different environments for gelatin-based films, revealed a positive responsive relative humidity behavior of materials and a sensibility of 99.47% for hybrid materials, suggesting the development of a new promising nanocomposite material for monitoring relative humidity conditions at 25 °C.
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References
Agmon, N. (1995). The grotthuss mechanism. Chemical Physics Letters, 244(5–6), 456–462. https://doi.org/10.1016/0009-2614(95)00905-J.
Alexandre, E. M. C., Lourenço, R. V., Bittante, A. M. Q. B., Moraes, I. C. F., & Sobral, P. J. A. (2016). Gelatin-based films reinforced with montmorillonite and activated with nanoemulsion of ginger essential oil for food packaging applications. Food Packaging and Shelf Life, 10, 87–96. https://doi.org/10.1016/j.fpsl.2016.10.0042214-2894.
Alshami, A. S., Tang, J., & Rasco, B. (2017). Contribution of proteins to the dielectric properties of dielectrically heated biomaterials. Food and Bioprocess Technology, 10(8), 1548–1561. https://doi.org/10.1007/s11947-017-1920-5.
Amjadi, S., Emaninia, S., Nazari, M., Davudian, S. H., & Roufegarinejad, L. (2019). Food and Bioprocess Technology, 12(7), 1205–1219. https://doi.org/10.1007/s11947-019-02286-y.
Arfat, Y. A., Benjakul, S., Prodpran, T., Sumpavapol, P., & Songtipya, P. (2016). Physico-mechanical characterization and antimicrobial properties of fish protein isolate/fish skin-zinc oxide (ZnO) nanocomposite films. Food and Bioprocess Technology, 9(1), 101–112. https://doi.org/10.1007/s11947-015-1602-0.
ASTM. (2016). Standard test methods for water vapor transmission of materials E96/E96M-16. Annual book of ASTM. Philadelphia: American Society for Testing and Materials.
Aziz, S. B., Karim, W. O., Brza, M. A., Abdulwahid, R. T., Saeed, S. R., Al-Zangana, S., & Kadir, M. F. Z. (2019). Ion transport study in CS: POZ based polymer membrane electrolytes using Trukhan model. International Journal of Molecular Sciences, 20(21), 5265. https://doi.org/10.3390/ijms20215265.
Barth, A. (2007). Infrared spectroscopy of proteins. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1767(9), 1073–1101. https://doi.org/10.1016/j.bbabio.2007.06.004.
Basavegowda, N., Mandal, T. K., & Baek, K. H. (2019). Bimetallic and trimetallic nanoparticles for active food packaging applications: a review. Food and Bioprocess Technology, 13(1), 1–15. https://doi.org/10.1007/s11947-019-02370-3.
Bergo, P., & Sobral, P. J. A. (2007). Effects of plasticizer on physical properties of pigskin gelatin films. Food Hydrocolloids, 21(8), 1285–1289. https://doi.org/10.1016/j.foodhyd.2006.09.014.
Bibi, F., Villain, M., Guillaume, C., Sorli, B., & Gontard, N. (2016). A review: origins of the dielectric properties of proteins and potential development as bio-sensors. Sensors, 16(8), 1232. https://doi.org/10.3390/s16081232.
Bibi, F., Guillaume, C., Gontard, N., & Sorli, B. (2017). Wheat gluten, a bio-polymer to monitor carbon dioxide in food packaging: electric and dielectric characterization. Sensors and Actuators B: Chemical, 250, 76–84. https://doi.org/10.1016/j.snb.2017.03.164.
Bodeux, R., Rousset, J., Tsin, F., Mollica, F., Leite, E., & Delbol, S. (2018). Determination of the electronic properties of Cu2ZnSnSe4-based solar cells by impedance spectroscopy and current-voltage characteristics analysis. Applied Physics A, 124(22). https://doi.org/10.1007/s00339-017-1437-9.
Chankhanittha, T., & Nanan, S. (2018). Hydrothermal synthesis, characterization and enhanced photocatalytic performance of ZnO toward degradation of organic azo dye. Materials Letters, 226, 79–82. https://doi.org/10.1016/j.matlet.2018.05.032.
Chethan, B., Prakash, H. R., Ravikiran, Y. T., Vijayakumari, S. C., Ramana, C. V., Thomas, S., & Kim, D. (2019). Enhancing humidity sensing performance of polyaniline/water soluble graphene oxide composite. Talanta, 196, 337–344. https://doi.org/10.1016/j.talanta.2018.12.072.
Ejaz, M., Arfat, Y. A., Mulla, M., & Ahmed, J. (2018). Zinc oxide nanorods/clove essential oil incorporated type B gelatin composite films and its applicability for shrimp packaging. Food Packaging and Shelf Life, 15, 113–121. https://doi.org/10.1016/j.fpsl.2017.12.004.
Espitia, P. J. P., Soares, N. F. F., Coimbra, J. S. R., Andrade, N. J., Cruz, R. S., & Medeiros, E. A. A. (2012). Zinc oxide nanoparticles: synthesis, antimicrobial activity and food packaging applications. Food and Bioprocess Technology, 5(5), 1447–1464. https://doi.org/10.1007/s11947-012-0797-6.
Gaikwad, K. K., Singh, S., & Ajji, A. (2019). Moisture absorbers for food packaging applications. Environmental Chemistry Letters, 17(2), 609–628. https://doi.org/10.1007/s10311-018-0810-z.
Ge, L., Zhu, M., Xu, Y., Li, X., Li, D., & Mu, C. (2017). Development of antimicrobial and controlled biodegradable gelatin-based edible films containing nisin and amino-functionalized montmorillonite. Food and Bioprocess Technology, 10(9), 1727–1736. https://doi.org/10.1007/s11947-017-1941-0.
Gontard, N., Guilbert, S., & Cuq, J. L. (1992). Edible wheat gluten films: influence of the main process variables on film properties using response surface methodology. Journal of Food Science, 57(1), 190–195. https://doi.org/10.1111/j.1365-2621.1992.tb05453.x.
Greenspan, L. (1977). Humidity fixed points of binary saturated aqueous solutions. Journal of Research of the National Bureau of Standards Section A: Physics and Chemistry, 81A(1), 89.
Gupta, A., & Srivastava, R. (2018). Zinc oxide nanoleaves: a scalable disperser-assisted sonochemical approach for synthesis and an antibacterial application. Ultrasonics Sonochemistry, 41, 47–58. https://doi.org/10.1016/j.ultsonch.2017.09.029.
Hamouche, H., Makhlouf, S., Chaouchi, A., & Laghrouche, M. (2018). Humidity sensor based on keratin bio polymer film. Sensors and Actuators A: Physical, 282, 132–141. https://doi.org/10.1016/j.sna.2018.09.025.
He, Q., Zhang, Y., Cai, X., & Wang, S. (2016). Fabrication of gelatin–TiO2 nanocomposite film and its structural, antibacterial and physical properties. International Journal of Biological Macromolecules, 84, 153–160. https://doi.org/10.1016/j.ijbiomac.2015.12.012.
Hoque, M. S., Benjakul, S., & Prodpran, T. (2011). Effects of partial hydrolysis and plasticizer content on the properties of film from cuttlefish (Sepia pharaonis) skin gelatin. Food Hydrocolloids, 25(1), 82–90. https://doi.org/10.1016/j.foodhyd.2010.05.008.
Kanmani, P., & Rhim, J. W. (2014a). Physical, mechanical and antimicrobial properties of gelatin based active nanocomposite films containing AgNPs and nanoclay. Food Hydrocolloids, 35, 644–652. https://doi.org/10.1016/j.foodhyd.2013.08.011.
Kanmani, P., & Rhim, J. W. (2014b). Properties and characterization of bionanocomposite films prepared with various biopolymers and ZnO nanoparticles. Carbohydrate Polymers, 106, 190–199. https://doi.org/10.1016/j.carbpol.2014.02.007.
Kathiravan, D., Huang, B. R., & Saravanan, A. (2017). Multifunctional sustainable materials: the role of carbon existing protein in the enhanced gas and UV sensing performances of ZnO-based biofilms. Journal of Materials Chemistry C, 5(21), 5239–5247. https://doi.org/10.1039/C7TC01305A.
Kokoszka, S., Debeaufort, F., Lenart, A., & Voilley, A. (2010). Water vapour permeability, thermal and wetting properties of whey protein isolate based edible films. International Dairy Journal, 20(1), 53–60. https://doi.org/10.1016/j.idairyj.2009.07.008.
Kotresh, S., Ravikiran, Y. T., Prakash, H. R., Ramana, C. V., Vijayakumari, S. C., & Thomas, S. (2016). Humidity sensing performance of spin coated polyaniline–carboxymethyl cellulose composite at room temperature. Cellulose, 23(5), 3177–3186. https://doi.org/10.1007/s10570-016-1035-6.
Liu, Y., Liu, X., & Wang, X. (2011). Biomimetic synthesis of gelatin polypeptide-assisted noble-metal nanoparticles and their interaction study. Nanoscale Research Letters, 6(1), 22. https://doi.org/10.1007/s11671-010-9756-1.
Liu, F., Chiou, B. S., Avena-Bustillos, R. J., Zhang, Y., Li, Y., McHugh, T. H., & Zhong, F. (2017). Study of combined effects of glycerol and transglutaminase on properties of gelatin films. Food Hydrocolloids, 65, 1–9. https://doi.org/10.1016/j.foodhyd.2016.10.004.
Mahapure, P. D., Gangal, S. A., Aiyer, R. C., & Gosavi, S. W. (2019). Combination of polymeric substrates and metal–polymer nanocomposites for optical humidity sensors. Journal of Applied Polymer Science, 136(5), 47035. https://doi.org/10.1002/app.47035.
Nafchi, A. M., Moradpour, M., Saeidi, M., & Alias, A. K. (2014). Effects of nanorod-rich ZnO on rheological, sorption isotherm, and physicochemical properties of bovine gelatin films. LWT- Food Science and Technology, 58(1), 142–149. https://doi.org/10.1016/j.lwt.2014.03.007.
Park, S., Lee, D., Kwak, B., Lee, H. S., Lee, S., & Yoo, B. (2018). Synthesis of self-bridged ZnO nanowires and their humidity sensing properties. Sensors and Actuators B: Chemical, 268, 293–298. https://doi.org/10.1016/j.snb.2018.04.118.
Patil, S., Ramgir, N., Mukherji, S., & Rao, V. R. (2017). PVA modified ZnO nanowire based microsensors platform for relative humidity and soil moisture measurement. Sensors and Actuators B: Chemical, 253, 1071–1078. https://doi.org/10.1016/j.snb.2017.07.053.
Peng, X., Chu, J., Aldalbahi, A., Rivera, M., Wang, L., Duan, S., & Feng, P. (2016). A flexible humidity sensor based on KC–MWCNTs composites. Applied Surface Science, 387, 149–154. https://doi.org/10.1016/j.apsusc.2016.05.108.
Pourrahimi, A. M., Liu, D., Pallon, L. K., Andersson, R. L., Abad, A. M., Lagaron, J. M., et al. (2014). Water-based synthesis and cleaning methods for high purity ZnO nanoparticles–comparing acetate, chloride, sulphate and nitrate zinc salt precursors. RSC Advances, 4(67), 35568–35577. https://doi.org/10.1039/C4RA06651K.
Pradhan, D. K., Choudhary, R. N. P., Samantaray, B. K., Karan, N. K., & Katiyar, R. S. (2007). Effect of plasticizer on structural and electrical properties of polymer nanocompsoite electrolytes. International Journal of Electrochemical Science, 2, 861–871.
Reddy, K. M., Feris, K., Bell, J., Wingett, D. G., Hanley, C., & Punnoose, A. (2007). Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systems. Applied Physics Letters, 90(21), 213902. https://doi.org/10.1063/1.2742324.
Rivero, S., García, M. A., & Pinotti, A. (2010). Correlations between structural, barrier, thermal and mechanical properties of plasticized gelatin films. Innovative Food Science & Emerging Technologies, 11(2), 369–375. https://doi.org/10.1016/j.ifset.2009.07.005.
Rouhi, J., Mahmud, S., Naderi, N., Ooi, C. R., & Mahmood, M. R. (2013). Physical properties of fish gelatin-based bio-nanocomposite films incorporated with ZnO nanorods. Nanoscale Research Letters, 8(1), 364. https://doi.org/10.1186/1556-276X-8-364.
Sahraee, S., Ghanbarzadeh, B., Milani, J. M., & Hamishehkar, H. (2017). Development of gelatin bionanocomposite films containing chitin and ZnO nanoparticles. Food and Bioprocess Technology, 10(8), 1441–1453. https://doi.org/10.1007/s11947-017-1907-2.
Sattar, M. A., Gangadharan, S., & Patnaik, A. (2019). Design of dual hybrid network natural rubber–SiO2 elastomers with tailored mechanical and self-healing properties. ACS Omega, 4(6), 10939–10949. https://doi.org/10.1021/acsomega.9b01243.
Shankar, S., & Rhim, J. W. (2019). Effect of types of zinc oxide nanoparticles on structural, mechanical and antibacterial properties of poly (lactide)/poly (butylene adipate-co-terephthalate) composite films. Food Packaging and Shelf Life, 21, 100327. https://doi.org/10.1016/j.fpsl.2019.100327.
Shankar, S., Teng, X., Li, G., & Rhim, J. W. (2015). Preparation, characterization, and antimicrobial activity of gelatin/ZnO nanocomposite films. Food Hydrocolloids, 45, 264–271. https://doi.org/10.1016/j.foodhyd.2014.12.001.
Shankar, S., Wang, L. F., & Rhim, J. W. (2019). Effect of melanin nanoparticles on the mechanical, water vapor barrier, and antioxidant properties of gelatin-based films for food packaging application. Food Packaging and Shelf Life, 21, 100363. https://doi.org/10.1016/j.fpsl.2019.100363.
Shukla, S. K., Kushwaha, C. S., Shukla, A., & Dubey, G. C. (2018). Integrated approach for efficient humidity sensing over zinc oxide and polypyrole composite. Materials Science and Engineering: C, 90, 325–332. https://doi.org/10.1016/j.msec.2018.04.054.
Teixeira Silva, F., Sorli, B., Calado, V., Guillaume, C., & Gontard, N. (2016). Feasibility of a gelatin temperature sensor based on electrical capacitance. Sensors, 16(12), 2197. https://doi.org/10.3390/s16122197.
Voon, H. C., Bhat, R., Easa, A. M., & Karim, L. A. A. (2012). Effect of addition of halloysite nanoclay and SiO2 nanoparticles on barrier and mechanical properties of bovine gelatin films. Food and Bioprocess Technology, 5(5), 1766–1774. https://doi.org/10.1007/s11947-010-0461-y.
Yadav, B., Kumar, R., Kumar, R., Chaudhuri, S., & Pramanik, P. (2017). Electrical behaviour of chitosan-silver nanocomposite in presence of water vapour. Journal of Water and Environmental Nanotechnology, 2(2), 71–79. https://doi.org/10.22090/JWENT.2017.02.001.
Zhang, Y., Yu, K., Jiang, D., Zhu, Z., Geng, H., & Luo, L. (2005). Zinc oxide nanorod and nanowire for humidity sensor. Applied Surface Science, 242(1–2), 212–217. https://doi.org/10.1016/j.apsusc.2004.08.013.
Zhu, L., & Zeng, W. (2017). Room-temperature gas sensing of ZnO-based gas sensor: a review. Sensors and Actuators A: Physical, 267, 242–261. https://doi.org/10.1016/j.sna.2017.10.021.
Acknowledgments
The authors are grateful to Gelita do Brasil LTDA for the donation of the gelatin.
Funding
This work was supported by CAPES (Finance code 001), CNPq (grant numbers 141938/2019-6, 305356/2016-0, 408330/2016-3, and 310659/2018-3), and FAPERJ (grant numbers E-26/202.710/2019 and E-26/203.005/2017) (Brazil).
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Pereira, P.F.M., Picciani, P.H.S., Calado, V.M.A. et al. Gelatin-Based Nanobiocomposite Films as Sensitive Layers for Monitoring Relative Humidity in Food Packaging. Food Bioprocess Technol 13, 1063–1073 (2020). https://doi.org/10.1007/s11947-020-02462-5
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DOI: https://doi.org/10.1007/s11947-020-02462-5