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New method of injecting solution into the abdomen to overcome fixation delay of the midgut on making the histological specimens of honeybee worker

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Abstract

Few histological studies have been performed to evaluate infectious diseases in honeybees, as their water-resistant exoskeletons make the preparation of whole-body specimens difficult by blocking the permeation of fixative and substitution solutions. Additionally, it remains difficult to fix samples when specimens are collected far from the laboratory, as delays in fixation lead to the autolysis of abdominal organs. In this study, we propose an intraperitoneal fixative injection method to prevent fixation delay and a method for preparing honeybee tissue specimens for histopathological examinations.

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Acknowledgements

This work was supported by a grant from Ministry of Agriculture, Forestry and Fisheries of Japan (Research project for Monitoring and enhancement of pollinators for crop production).

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Authors and Affiliations

  1. Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo, Motoyama, Kita-ku, Kyoto, Japan

    Tetsuro Inamoto, Jonosuke Mizobata & Junichi Takahashi

  2. Kyoto Prefecture Rural Development Division, Department of Agriculture, Yabunouchi-cho, Shinmachi, Shimodachiuri-Dori, Kamigyo-ku, Kyoto, Japan

    Tetsuro Inamoto

  3. Department of Animal Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, Japan

    Youhei Mantani, Toshifumi Yokoyama, Nobuhiko Hoshi & Hiroshi Kitagawa

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  1. Tetsuro Inamoto
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  2. Jonosuke Mizobata
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  3. Youhei Mantani
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  4. Toshifumi Yokoyama
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  5. Nobuhiko Hoshi
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  6. Hiroshi Kitagawa
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  7. Junichi Takahashi
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Correspondence to Junichi Takahashi.

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Inamoto, T., Mizobata, J., Mantani, Y. et al. New method of injecting solution into the abdomen to overcome fixation delay of the midgut on making the histological specimens of honeybee worker. Appl Entomol Zool 55, 351–354 (2020). https://doi.org/10.1007/s13355-020-00688-5

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  • DOI: https://doi.org/10.1007/s13355-020-00688-5

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