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LPS-induced inflammation disorders bone modeling and remodeling by inhibiting angiogenesis and disordering osteogenesis in chickens

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Abstract

Inflammation plays a negative role in the growth and development of bone. However, the underlining mechanisms of inflammation caused abnormal bone development and even bone disease are still poorly understood, especially in chickens. In this study, we explored the influence of inflammation on bone formation in broilers for the first time by using lipopolysaccharide (LPS) to establish systemic inflammatory models in chickens with tibia as the research object. The measurements of production and tibial parameters showed an inefficient production performance and lower growth rate in LPS group. We also found a large amount of platelets, inflammatory cells in chickens’ blood and higher levels of inflammatory factors in serum after LPS injection, meanwhile, increase in thrombus, chondrocyte nucleolysis, and osteoclasts and a reduction in blood vessels were observed in growth plate through histological observation. The qPCR analysis showed that the mRNA expression levels of NF-κB, TLR4, TF, TPO, and its receptor C-MPL enhanced, while VEGFA was inhibited in LPS group. In addition, in OPG/RANKL system, OPG was decreased while RANKL enhanced. It was also observed that the mRNA levels of MMP-9 and its inducing factor CD147 enhanced in LPS group. The western blot results were basically in consistent with mRNA test. Thus, we infer that inflammation can inhibit bone modeling and remodeling by affecting angiogenesis and osteogenesis, and result in negative effect on bone formation furtherly.

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Acknowledgments

This study was supported by National Key Research and Development Program of China (Project No. 2017YFD0502200) and the National Natural Science Foundation of China (No. 31460682).

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Tibet Agricultural and Animal Husbandry College, Linzhi, 860000, Tibet, People’s Republic of China

    Xiaole Tong & Jiakui Li

  2. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Xiaole Tong, Jialu Zhang & Jiakui Li

Authors
  1. Xiaole Tong
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  2. Jialu Zhang
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  3. Jiakui Li
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Contributions

XT and JL provided the research idea. XT and JZ conducted the experiments. XT and JZ analyzed the data. XT wrote the manuscript and JL had the primary responsibility for the final content. All authors contributed to the final manuscript.

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Correspondence to Jiakui Li.

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The authors declare that they have no conflict of interest.

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Tong, X., Zhang, J. & Li, J. LPS-induced inflammation disorders bone modeling and remodeling by inhibiting angiogenesis and disordering osteogenesis in chickens. Inflamm. Res. 69, 765–777 (2020). https://doi.org/10.1007/s00011-020-01361-x

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  • DOI: https://doi.org/10.1007/s00011-020-01361-x

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