Abstract
Background
Viral hemorrhagic septicemia (VHS) is a serious viral disease that infects the olive flounder in South Korea. The Korean aquaculture industry experienced an economic loss caused by the high infectivity and mortality.
Objective
This study aimed to evaluate the infection density of VHSV in various organs of the olive flounder including spleen, liver, kidney, stomach, esophagus, intestine, gill, muscle, heart, and brain. Olive flounders were collected from a local fish farm and injected subcutaneously with 106 PFU/fish.
Methods
Each 15 fish were sampled at 0, 3, and 7 days post challenge (dpc), respectively, to perform quantitative analysis of VHSV using SYBR-green based real-time PCR in various tissues including spleen, liver, head-kidney, body-kidney, muscle, esophagus, stomach, intestine, gill, and brain.
Results
Organs infected with VHSV were obtained after 3 and 7 days. Each organs were examined for viral infection using real-time PCR. The data obtained from this experiment revealed copy numbers higher than 10 copies per 100 ng cDNA in the spleen (15.26 ± 3.11 copies/100 ng of cDNA), muscle (11.24 ± 2.25 copies), and gill (14.23 ± 6.26 copies), but lower in liver, head-kidney, body-kidney, esophagus, brain and stomach.
Conclusion
The present study, together with previous data, demonstrated that the gill, spleen, and muscle are the major target organs of VHSV in olive flounder. Therefore, central monitoring of spleen, gill and muscle should be considered and might be necessary if anti-VHSV treatment is to be successful in infected olive flounder.
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Acknowledgements
This research was a part of a project titled ‘Omics based on fishery disease control technology development and industrialization (20150242),’ funded by the Ministry of Oceans and Fisheries, Korea.
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Hyunsu Kim declares that he has no conflict of interest. Kyung-Wan Baek declares that he has no conflict of interest. Eun-Ji Ko declares that he has no conflict of interest. Nguyen Thanh Luan declares that he has no conflict of interest. Yunjin Lim declares that he has no conflict of interest. Heyong Jin Roh declares that he has no conflict of interest. Nameun Kim declares that he has no conflict of interest. Do-Hyung Kim declares that he has no conflict of interest. Yung Hyun Choi declares that he has no conflict of interest. Suhkmann Kim declares that he has no conflict of interest. Heui-Soo Kim declares that he has no conflict of interest. Mee Sun Ock declares that he has no conflict of interest. Hee-Jae Cha declares that he has no conflict of interest.
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All experiments were carried out in accordance with the guidelines and regulation approved by Ethical Committee of Pukyong National University.
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Kim, H., Baek, KW., Ko, EJ. et al. Genome based quantification of VHSV in multiple organs of infected olive flounder (Paralichthys olivaceus) using real-time PCR. Genes Genom 42, 773–777 (2020). https://doi.org/10.1007/s13258-020-00951-7
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DOI: https://doi.org/10.1007/s13258-020-00951-7