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The role of CTNNB1 and LEF1 in feather follicles development of Anser cygnoides and Anser anser

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Abstract

Background

Wingless-types/beta-catenin (Wnt/β-catenin) signaling pathway is one of the most extensively studied transcriptional cascades involved in various types of organogenesis including embryonic and postnatal development. Downy feather quantity is primarily affected by follicular development and gene regulations.

Objective

This research was aimed to investigate the role of catenin beta-1(CTNNB1) and lymphoid enhancerbinding factor-1 (LEF1) on feather follicles development at different developmental stages.

Methods

Fluorescence quantitative PCR, Western-blot and immunohistochemical methods were used in Anser cygnoides and Anser anser embryos (E12, E13 E18, and E28) and after birth gosling stages (G18, G48, G88) for gene expression analysis.

Results

CTNNB1 and LEF1 genes were expressed in Anser cygnoides and Anser anser at different embryonic and after-birth gosling developmental stages and the expression levels were significantly different in different stages (p < 0.05). The mRNA expression of CTNNB1 and LEF1 genes reached the highest level at D88 in Anser cygnoides, while the highest expression levels were at D18 and D88 in Anser anser, and the expression levels of CTNNB1 genes at D88 in all embryonic stages were significantly lower than after-birth stages. CTNNB1 and LEF1 protein expression were the highest at E12 and E28 for Anser cygnoides feather follicles development. While at a similar stage for Anser anser, the expression of CTNNB1 and LEF1 protein was the highest at D48 and D18. Protein expression at embryonic stages was in the epidermis (E) and the hair basal plate (P), the expression site for after-birth stages was in the dermal papilla (DP).

Conclusion

Our study illustrated that CTNNB1 and LEF1 has an impact on Anser cygnoides and Anser anser feather follicles growth and development.

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Acknowledgements

Authors appreciate the Jilin Agricultural University Geese Farm for daily management to raise geese including the incubation system operations.

Funding

This research was funded by the science and technology talent project in special fund for science and technology innovation of Jilin provincial science and technology department (2020), the Jilin native high-quality poultry breeds reproduction and extension (201904), and the Jilin Province Science and Technology Development Project (20180201034NY).

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Author notes

  1. Yue Sun, Yuxuan Zhou and Petunia Msuthwana contributed equally to this work.

Authors and Affiliations

  1. Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street, No: 2888, Changchun, 130118, People’s Republic of China

    Yue Sun, Yuxuan Zhou, Petunia Msuthwana, Jing Liu, Chang Liu, Cornelius Tlotliso Sello, Yupu Song, Ziqiang Feng, Shengyi Li, Wei Yang, Yunpeng Xu, Xiaomin Yan, Chuanghang Li, Yujian Sui, Jingtao Hu & Yongfeng Sun

  2. Key Laboratory for Animal Production, Product Quality and Safety of Ministry of Education, Changchun, People’s Republic of China

    Yongfeng Sun

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  1. Yue Sun
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Correspondence to Yongfeng Sun.

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Sun, Y., Zhou, Y., Msuthwana, P. et al. The role of CTNNB1 and LEF1 in feather follicles development of Anser cygnoides and Anser anser. Genes Genom 42, 761–771 (2020). https://doi.org/10.1007/s13258-020-00950-8

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