Abstract
RNA editing is a posttranscriptional molecular process involved with specific nucleic modification, which can enhance the diversity of gene products. Adenosine-to-inosine (A-to-I, I is read as guanosine by both splicing and translation machinery) is the main type of RNA editing in mammals, which manifested as AG (adenosine-to-guanosine) in sequence data. Here, we aimed to explore patterns of RNA editing using RNA sequencing data from skeletal muscle at four developmental stages (three fetal periods and one postnatal period) in goat. We found the occurrences of RNA editing events raised at fetal periods and declined at the postnatal period. Also, we observed distinct editing levels of AG editing across stages, and significant difference was found between postnatal period and fetal periods. AG editing patterns in newborn goats are similar to those of 45-day embryo compared with embryo at 105 days and embryo at 60 days. In this study, we found a total of 1415 significantly differential edited AG sites among four groups. Moreover, 420 sites were obviously clustered into six time-series profiles, and one profile had significant association between editing level and gene expression. Our findings provided some novel insights into understanding the molecular mechanism of muscle development in mammals.
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Acknowledgments
The authors thank Xiaoli Xu, Juntao Li, Yuan Chen, and Jiangxue Cao for their comments on earlier drafts of the manuscript. The authors are also grateful for the critical comments of anonymous reviewers.
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This work was supported by the National Key Research and Development Program of China (2018YFD0502002), National Natural Science Foundation of China (31672402), and Chengdu City Science and Technology Support Program (2018-YF09-00017-SN).
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LY and BK drafted the manuscript and performed the experiments. LJW, TZ, SYZ, and WZ collected the blood samples and exacted DNA samples. LY and YMS performed data analysis. LYX and LL conceived of the study. HPZ and LL participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
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Yang, L., Li, L., Kyei, B. et al. Systematic analyses reveal RNA editing events involved in skeletal muscle development of goat (Capra hircus). Funct Integr Genomics 20, 633–643 (2020). https://doi.org/10.1007/s10142-020-00741-0
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DOI: https://doi.org/10.1007/s10142-020-00741-0