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Morphology and composition of the midgut bacterial community of Scaptocoris castanea Perty, 1830 (Hemiptera: Cydnidae)

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Abstract

The burrower bug Scaptocoris castanea is an important soybean and pasture pest in Brazil, with an underground habit feeding directly on the sap of the roots. Underground habit hinders control and knowledge of the biology and physiology of this pest. This study describes the anatomy, histology, ultrastructure and symbionts of the midgut of S. castanea. The midgut of S. castanea is anatomically divided into five regions (ventricles). Ventricles 1–3 are similar between males and females, with cells specialized in digestion and absorption of nutrients, water transport and homeostasis. Ventricle 4 has squamous epithelium forming crypts and harboring bacteria in the lumen. Ventricle 5 of males is small with cells containing apical microvilli and broad basal folds with many openings for hemocoel, while in females, this region of the midgut is well developed and colonized by intracellular bacteria, characterizing bacteriocytes. The main bacteria are Gammaproteobacteria. The results show sexual dimorphism in ventricle 5 of the midgut of S. castanea, with formation of bacteriocytes in the females, while the other regions are involved in digestive processes in both sexes.

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Acknowledgments

The authors thank José Carlos Gomes de Araújo, Gabriel dos Santos Carvalho and Fabiano Gonçalves Schimmer for their help in collecting insects on soybean crops; Dr. Lucia M. Vivan for identifying the insects; and Dr. André Rodrigues for photographs of the gut anatomy. We thank Dr. Luanda M. Santana for the revision of the manuscript. We are grateful to the Nucleus of Microscopy and Microanalysis of the Federal University of Viçosa for technical support.

Funding

This research was supported by the Brazilian research agencies National Council for Scientific and Technological Development (CNPq), Coordination of Improvement of Higher Education Personnel (CAPES), and Research Support Foundation of the State of Minas Gerais (FAPEMIG).

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Authors and Affiliations

  1. Departamento de Biologia Geral, Universidade Federal de Viçosa, Vicosa, Minas Gerais, 36570-000, Brazil

    Jamile Fernanda Silva Cossolin, Luis Carlos Martínez & José Eduardo Serrão

  2. Laboratório de Microbiologia de Anaeróbios, Departamento de Microbiologia, Universidade Federal de Viçosa, Vicosa, Minas Gerais, 36570-000, Brazil

    Déborah Romaskevis Gomes Lopes & Hilário Cuquetto Mantovani

  3. Instituto Federal de Educação, Ciência e Tecnologia de Minas Gerais IFMG - Campus Congonhas, Congonhas, Minas Gerais, 36415-000, Brazil

    Helen Cristina Pinto Santos

  4. Departamento de Entomologia, Universidade Federal de Viçosa, Vicosa, Minas Gerais, 36570-000, Brazil

    Muhammad Fiaz

  5. Laboratório de Entomologia, Universidade do Estado de Mato Grosso, Tangara da Serra, Mato Grosso, 78300-000, Brazil

    Mônica Josene Barbosa Pereira

  6. Fundação de Apoio a Pesquisa Agropecuária de Mato Grosso, Rondonopolis, MT, 78750-360, Brazil

    Lucia Madalena Vivan

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  1. Jamile Fernanda Silva Cossolin
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Correspondence to José Eduardo Serrão.

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Cossolin, J.F.S., Lopes, D.R.G., Martínez, L.C. et al. Morphology and composition of the midgut bacterial community of Scaptocoris castanea Perty, 1830 (Hemiptera: Cydnidae). Cell Tissue Res 382, 337–349 (2020). https://doi.org/10.1007/s00441-020-03197-7

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