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Mitsuaria chitinivorans sp. nov. a potential candidate for bioremediation: emended description of the genera Mitsuaria, Roseateles and Pelomonas

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Abstract

As a part of studying the effect of deoxygenation, eutrophication and acidification on bacterial diversity, strain HWN-4T was isolated from tube well water and characterized. The draft genome sequencing of strain HWN-4T revealed a genome size of 5,774,764 bp and the annotation indicated 5102 coding sequences including 66 RNA genes. Strain HWN-4T is Gram negative, rod-shaped, motile in the log phase, catalase and oxidase positive, and the major fatty acids and respiratory quinone present are C10:0 3-OH, C14:0 3OH/C16:1 iso I, C16:1 ω7c/C16:1 ω6c, C16:0 and C17:0 cyclo and ubiquinone-8, respectively. The phylogenetic analyses, based on 16S rRNA gene sequence, indicated that strain HWN-4T is a member of the genus Mitsuaria. The average nucleotide identity (ANI) and genome-to-genome similarity between strain HWN-4T and all other species/strains of the genus Mitsuaria are less than (%) 95.0 and 70.0, respectively. This confirms the status of strain HWN-4T as a novel species. The species status is further confirmed by phenotypic differences exhibited by strain HWN-4T with other members of the same genus. Based on the collective differences exhibited by strain HWN-4T with other members of the genus Mitsuaria, the name Mitsuaria chitinivorans sp. nov. is proposed. Further, the diagnostic signature nucleotides were identified in the 16S rRNA gene sequences of members of the genera Mitsuaria, Pelomonas and Roseateles, that distinctly differentiate them and support an emendation of the genera. Besides, phylogenetic and structural characterization of chitinases from members of the genus Mitsuaria was performed. The type strain of Mitsuaria chitinivorans sp. nov. is HWN-4T = LMG 28685T = KTCC 42483T.

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Acknowledgements

Authors acknowledge Council for Scientific and Industrial Research (CSIR), India, for funding the project INDIA’s IDEA (PSC0108). We also thank Dr. SWA Naqvi, Director, CSIR-NIO, for coordinating the activities of the INDIA’s IDEA project.

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    Shivaji Sisinthy & Sathyanarayana Reddy Gundlapally

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The GenBank accession number for the 16S rRNA gene sequence and the draft genome sequence of Mitsuaria chitinivorans sp. nov. (HWN-4T) are MH077556 and PEOG00000000, respectively.

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Sisinthy, S., Gundlapally, S.R. Mitsuaria chitinivorans sp. nov. a potential candidate for bioremediation: emended description of the genera Mitsuaria, Roseateles and Pelomonas. Arch Microbiol 202, 1839–1848 (2020). https://doi.org/10.1007/s00203-020-01905-z

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