Abstract
This study was conducted to prepare β-caryophyllene loaded liposomes (BCP-LP) and investigated their effects on neurovascular unit (NVU) damage after subarachnoid hemorrhage (SAH) in rats. A blood injection into the pre-chiasmatic cistern was used to achieve SAH. BCP-LP were prepared, characterized and administrated to rats with SAH. The prepared BCP-LP were spherical with a size distribution of approximately 189.3 nm and Zeta potential of − 13.9 mV. Neurological scoring, the balance beam test, cerebral blood flow monitoring, brain edema and biochemical analyses were applied to evaluate the effects of BCP-LP on rat NVU damage after SAH. The results demonstrated that BCP-LP treatment improved neurological function disorder, balance ability and cerebral blood perfusion in rats. Brain edema detection and blood–brain barrier permeability detection revealed that BCP-LP could reduce brain edema and promote repairment of blood–brain barrier after SAH. Using the western blot experiments, we demonstrated that BCP-LP attenuated the loss of tight junction proteins Occludin and Zonula occludens-1, inhibit the high expression of VEGFR-2 and GFAP, and promote the repair of laminin. These results demonstrate the protective effect BCP-LP exert in the NVU after SAH in rats, and supports the use of BCP-LP for future study and therapy of SAH.
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Acknowledgements
The research presented in this manuscript was funded by Special project of incentives and guidance for scientific research institutions in Chongqing (cstc2018jxjl130049), Traditional Chinese Medicine Science and Technology Project of Chongqing municipal health and Health Committee (ZY201702014) and Special Project of Chengdu University of Traditional Chinese Medicine (YYZX20180045). We thank Bronwen Gardner, PhD, from Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.
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Wang, F., Teng, Z., Liu, D. et al. β-Caryophyllene Liposomes Attenuate Neurovascular Unit Damage After Subarachnoid Hemorrhage in Rats. Neurochem Res 45, 1758–1768 (2020). https://doi.org/10.1007/s11064-020-03037-8
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DOI: https://doi.org/10.1007/s11064-020-03037-8